Assessment of spermatogenic process by deoxyribonucleic acid image analysis
To evaluate the spermatogenic process through cellular ploidy by image analysis. Twenty-six testicular aspirates from 24 infertile men were examined by fine needle aspiration (FNA) cytologic smears. These results were compared with the ploidy content of the cells using Feulgen stain, determined by i...
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| Veröffentlicht in: | Fertility and sterility 1995-08, Vol.64 (2), p.403-407 |
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| creator | Gottschalk-Sabag, Shoshana Weiss, David B. Sherman, Yoav |
| description | To evaluate the spermatogenic process through cellular ploidy by image analysis.
Twenty-six testicular aspirates from 24 infertile men were examined by fine needle aspiration (FNA) cytologic smears. These results were compared with the ploidy content of the cells using Feulgen stain, determined by image analysis.
The results of both methods were divided into three categories: full spermatogenesis, spermatogenic arrest, and only Sertoli cell. There was a good correlation in 25 of 26 smears (96%). Ten patients who had a histogram of diploid showed only Sertoli cell cytologically. From nine patients who had a histogram of diploid and tetraploid, eight cytologically showed spermatogenic arrest and one showed full spermatogenesis. The seven patients who had full spermatogenesis (haploid, diploid, tetraploid) all had normal cytologic smears.
Deoxyribonucleic acid image analysis is an objective qualitative and quantitative method for the evaluation of the spermatogenic process of the infertile male. It has several advantages over the flow cytometry method. |
| doi_str_mv | 10.1016/S0015-0282(16)57742-5 |
| format | Article |
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Twenty-six testicular aspirates from 24 infertile men were examined by fine needle aspiration (FNA) cytologic smears. These results were compared with the ploidy content of the cells using Feulgen stain, determined by image analysis.
The results of both methods were divided into three categories: full spermatogenesis, spermatogenic arrest, and only Sertoli cell. There was a good correlation in 25 of 26 smears (96%). Ten patients who had a histogram of diploid showed only Sertoli cell cytologically. From nine patients who had a histogram of diploid and tetraploid, eight cytologically showed spermatogenic arrest and one showed full spermatogenesis. The seven patients who had full spermatogenesis (haploid, diploid, tetraploid) all had normal cytologic smears.
Deoxyribonucleic acid image analysis is an objective qualitative and quantitative method for the evaluation of the spermatogenic process of the infertile male. It has several advantages over the flow cytometry method.</description><identifier>ISSN: 0015-0282</identifier><identifier>EISSN: 1556-5653</identifier><identifier>DOI: 10.1016/S0015-0282(16)57742-5</identifier><identifier>PMID: 7615121</identifier><identifier>CODEN: FESTAS</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Biological and medical sciences ; Biopsy, Needle ; Birth control ; DNA - analysis ; Flow Cytometry ; FNA ; Gynecology. Andrology. Obstetrics ; Humans ; Image analysis ; Infertility, Male - diagnosis ; Male ; Medical sciences ; Spermatogenesis ; Sterility. Assisted procreation ; testis ; Testis - chemistry ; Testis - pathology</subject><ispartof>Fertility and sterility, 1995-08, Vol.64 (2), p.403-407</ispartof><rights>1995 American Society for Reproductive Medicine</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-20560865b751040e2d33b21c0f042fd19edaa26ee3e068bfd70df9c7a1f236073</citedby><cites>FETCH-LOGICAL-c389t-20560865b751040e2d33b21c0f042fd19edaa26ee3e068bfd70df9c7a1f236073</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0015-0282(16)57742-5$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3642207$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7615121$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gottschalk-Sabag, Shoshana</creatorcontrib><creatorcontrib>Weiss, David B.</creatorcontrib><creatorcontrib>Sherman, Yoav</creatorcontrib><title>Assessment of spermatogenic process by deoxyribonucleic acid image analysis</title><title>Fertility and sterility</title><addtitle>Fertil Steril</addtitle><description>To evaluate the spermatogenic process through cellular ploidy by image analysis.
Twenty-six testicular aspirates from 24 infertile men were examined by fine needle aspiration (FNA) cytologic smears. These results were compared with the ploidy content of the cells using Feulgen stain, determined by image analysis.
The results of both methods were divided into three categories: full spermatogenesis, spermatogenic arrest, and only Sertoli cell. There was a good correlation in 25 of 26 smears (96%). Ten patients who had a histogram of diploid showed only Sertoli cell cytologically. From nine patients who had a histogram of diploid and tetraploid, eight cytologically showed spermatogenic arrest and one showed full spermatogenesis. The seven patients who had full spermatogenesis (haploid, diploid, tetraploid) all had normal cytologic smears.
Deoxyribonucleic acid image analysis is an objective qualitative and quantitative method for the evaluation of the spermatogenic process of the infertile male. It has several advantages over the flow cytometry method.</description><subject>Biological and medical sciences</subject><subject>Biopsy, Needle</subject><subject>Birth control</subject><subject>DNA - analysis</subject><subject>Flow Cytometry</subject><subject>FNA</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Image analysis</subject><subject>Infertility, Male - diagnosis</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Spermatogenesis</subject><subject>Sterility. Assisted procreation</subject><subject>testis</subject><subject>Testis - chemistry</subject><subject>Testis - pathology</subject><issn>0015-0282</issn><issn>1556-5653</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1rGzEQhkVJSB23PyGwhxLSwyYjyZK8pxJCmpYackh7FlppFFT2w9WsQ_zvo9jG157E8D4zenkYu-BwzYHrmycArmoQS3HF9VdlzELU6gObcaV0rbSSJ2x2RD6yc6K_AKC5EWfszGiuuOAz9uuWCIl6HKZqjBWtMfduGp9xSL5a59GXsGq3VcDxdZtTOw4b32HJnE-hSr17xsoNrttSok_sNLqO8PPhnbM_3-9_3_2oV48PP-9uV7WXy2aqBSgNS61aozgsAEWQshXcQ4SFiIE3GJwTGlEi6GUbg4EQG28cj0JqMHLOLvd3S79_G6TJ9ok8dp0bcNyQNUY2DVeigGoP-jwSZYx2nUvlvLUc7LtEu5No3w3ZMu0kWlX2Lg4fbNoew3HrYK3kXw65I--6mN3gEx0xqRdC7Hp-22NYZLwkzJZ8wsFjSBn9ZMOY_lPkDTcFjgw</recordid><startdate>19950801</startdate><enddate>19950801</enddate><creator>Gottschalk-Sabag, Shoshana</creator><creator>Weiss, David B.</creator><creator>Sherman, Yoav</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19950801</creationdate><title>Assessment of spermatogenic process by deoxyribonucleic acid image analysis</title><author>Gottschalk-Sabag, Shoshana ; Weiss, David B. ; Sherman, Yoav</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-20560865b751040e2d33b21c0f042fd19edaa26ee3e068bfd70df9c7a1f236073</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Biological and medical sciences</topic><topic>Biopsy, Needle</topic><topic>Birth control</topic><topic>DNA - analysis</topic><topic>Flow Cytometry</topic><topic>FNA</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Image analysis</topic><topic>Infertility, Male - diagnosis</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Spermatogenesis</topic><topic>Sterility. Assisted procreation</topic><topic>testis</topic><topic>Testis - chemistry</topic><topic>Testis - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gottschalk-Sabag, Shoshana</creatorcontrib><creatorcontrib>Weiss, David B.</creatorcontrib><creatorcontrib>Sherman, Yoav</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Fertility and sterility</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gottschalk-Sabag, Shoshana</au><au>Weiss, David B.</au><au>Sherman, Yoav</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Assessment of spermatogenic process by deoxyribonucleic acid image analysis</atitle><jtitle>Fertility and sterility</jtitle><addtitle>Fertil Steril</addtitle><date>1995-08-01</date><risdate>1995</risdate><volume>64</volume><issue>2</issue><spage>403</spage><epage>407</epage><pages>403-407</pages><issn>0015-0282</issn><eissn>1556-5653</eissn><coden>FESTAS</coden><abstract>To evaluate the spermatogenic process through cellular ploidy by image analysis.
Twenty-six testicular aspirates from 24 infertile men were examined by fine needle aspiration (FNA) cytologic smears. These results were compared with the ploidy content of the cells using Feulgen stain, determined by image analysis.
The results of both methods were divided into three categories: full spermatogenesis, spermatogenic arrest, and only Sertoli cell. There was a good correlation in 25 of 26 smears (96%). Ten patients who had a histogram of diploid showed only Sertoli cell cytologically. From nine patients who had a histogram of diploid and tetraploid, eight cytologically showed spermatogenic arrest and one showed full spermatogenesis. The seven patients who had full spermatogenesis (haploid, diploid, tetraploid) all had normal cytologic smears.
Deoxyribonucleic acid image analysis is an objective qualitative and quantitative method for the evaluation of the spermatogenic process of the infertile male. It has several advantages over the flow cytometry method.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>7615121</pmid><doi>10.1016/S0015-0282(16)57742-5</doi><tpages>5</tpages></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Biological and medical sciences Biopsy, Needle Birth control DNA - analysis Flow Cytometry FNA Gynecology. Andrology. Obstetrics Humans Image analysis Infertility, Male - diagnosis Male Medical sciences Spermatogenesis Sterility. Assisted procreation testis Testis - chemistry Testis - pathology |
| title | Assessment of spermatogenic process by deoxyribonucleic acid image analysis |
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