Growth Stimulation by Transfection of Intestinal Epithelial Cells with an Antisense Insulin-like Growth Factor-Binding Protein-2 Construct
IEC-6 cells, an intestinal epithelial cell line, produce insulin-like growth factor (IGF)-II and IGF-binding protein-2 (IGFBP-2). Exogenous IGFs stimulate and IGFBP-2 attenuates DNA synthesis. To investigate whether endogenously secreted IGFBP-2 inhibits proliferation, IEC-6 cells were transfected w...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1995-06, Vol.211 (3), p.707-713 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Corkins, M.R. Vanderhoof, J.A. Slentz, D.H. Macdonald, R.G. Park, J.H.Y. |
| description | IEC-6 cells, an intestinal epithelial cell line, produce insulin-like growth factor (IGF)-II and IGF-binding protein-2 (IGFBP-2). Exogenous IGFs stimulate and IGFBP-2 attenuates DNA synthesis. To investigate whether endogenously secreted IGFBP-2 inhibits proliferation, IEC-6 cells were transfected with a full-length rat IGFBP-2 cDNA antisense expression construct. The steady-state level of IGFBP-2 mRNA decreased by 54% in the antisense cDNA-transfected cells (denoted revBP2) compared with vector-transfected controls. Moreover, revBP2 cells secreted less IGFBP-2 than controls (maximally a 68% decrease). In serum-containing medium, revBP2 cells exhibited a 35% increase in log-phase proliferation rate and growth-arrested at a maximum density 14% higher than controls. We conclude that endogenous IGFBP-2 inhibits proliferation of IEC-6 cells, probably by sequestering IGFs. |
| doi_str_mv | 10.1006/bbrc.1995.1870 |
| format | Article |
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Exogenous IGFs stimulate and IGFBP-2 attenuates DNA synthesis. To investigate whether endogenously secreted IGFBP-2 inhibits proliferation, IEC-6 cells were transfected with a full-length rat IGFBP-2 cDNA antisense expression construct. The steady-state level of IGFBP-2 mRNA decreased by 54% in the antisense cDNA-transfected cells (denoted revBP2) compared with vector-transfected controls. Moreover, revBP2 cells secreted less IGFBP-2 than controls (maximally a 68% decrease). In serum-containing medium, revBP2 cells exhibited a 35% increase in log-phase proliferation rate and growth-arrested at a maximum density 14% higher than controls. We conclude that endogenous IGFBP-2 inhibits proliferation of IEC-6 cells, probably by sequestering IGFs.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.1995.1870</identifier><identifier>PMID: 7541197</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Carrier Proteins - genetics ; Carrier Proteins - secretion ; Cell Division - drug effects ; Cells, Cultured ; DNA, Antisense - pharmacology ; Epithelial Cells ; Epithelium - drug effects ; Epithelium - growth & development ; Insulin-Like Growth Factor Binding Protein 2 ; Insulin-Like Growth Factor II - metabolism ; Intestines - cytology ; Intestines - drug effects ; Intestines - growth & development ; Rats ; RNA, Messenger - analysis ; Somatomedins - genetics ; Somatomedins - secretion ; Transfection</subject><ispartof>Biochemical and biophysical research communications, 1995-06, Vol.211 (3), p.707-713</ispartof><rights>1995 Academic Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c339t-268973393e4bbff5f2ce07e3323ba829cac890106cfb2f432d5508ecf20d4a723</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/bbrc.1995.1870$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27907,27908,45978</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7541197$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Corkins, M.R.</creatorcontrib><creatorcontrib>Vanderhoof, J.A.</creatorcontrib><creatorcontrib>Slentz, D.H.</creatorcontrib><creatorcontrib>Macdonald, R.G.</creatorcontrib><creatorcontrib>Park, J.H.Y.</creatorcontrib><title>Growth Stimulation by Transfection of Intestinal Epithelial Cells with an Antisense Insulin-like Growth Factor-Binding Protein-2 Construct</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>IEC-6 cells, an intestinal epithelial cell line, produce insulin-like growth factor (IGF)-II and IGF-binding protein-2 (IGFBP-2). Exogenous IGFs stimulate and IGFBP-2 attenuates DNA synthesis. To investigate whether endogenously secreted IGFBP-2 inhibits proliferation, IEC-6 cells were transfected with a full-length rat IGFBP-2 cDNA antisense expression construct. The steady-state level of IGFBP-2 mRNA decreased by 54% in the antisense cDNA-transfected cells (denoted revBP2) compared with vector-transfected controls. Moreover, revBP2 cells secreted less IGFBP-2 than controls (maximally a 68% decrease). In serum-containing medium, revBP2 cells exhibited a 35% increase in log-phase proliferation rate and growth-arrested at a maximum density 14% higher than controls. We conclude that endogenous IGFBP-2 inhibits proliferation of IEC-6 cells, probably by sequestering IGFs.</description><subject>Animals</subject><subject>Carrier Proteins - genetics</subject><subject>Carrier Proteins - secretion</subject><subject>Cell Division - drug effects</subject><subject>Cells, Cultured</subject><subject>DNA, Antisense - pharmacology</subject><subject>Epithelial Cells</subject><subject>Epithelium - drug effects</subject><subject>Epithelium - growth & development</subject><subject>Insulin-Like Growth Factor Binding Protein 2</subject><subject>Insulin-Like Growth Factor II - metabolism</subject><subject>Intestines - cytology</subject><subject>Intestines - drug effects</subject><subject>Intestines - growth & development</subject><subject>Rats</subject><subject>RNA, Messenger - analysis</subject><subject>Somatomedins - genetics</subject><subject>Somatomedins - secretion</subject><subject>Transfection</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1vEzEQhi1E1YbClVsln7htGNu78fpYon5JlUBqkbhZXu-Ymm7s1PZS9S_wq-uQiBsnz2gez-h9CPnIYMkAVp-HIdklU6pbsl7CG7JgoKDhDNq3ZAGVaLhiP07Iu5x_ATDWrtQxOZZdy5iSC_LnKsXn8kDvit_Mkyk-Bjq80PtkQnZo__bR0ZtQMBcfzEQvtr484ORrucZpyvS59tQEeh6KzxgyVjrPkw_N5B-RHg5cGltiar74MPrwk35LsWBFOF3HkEuabXlPjpyZMn44vKfk--XF_fq6uf16dbM-v22sEKo0fNUrWSuB7TA41zluESQKwcVgeq6ssb0CBivrBu5awceugx6t4zC2RnJxSj7t925TfJprLL3x2dYoJmCcs5ZSqJaDrOByD9oUc07o9Db5jUkvmoHeydc7-XonX-_k1w9nh83zsMHxH36wXef9fo413m-PSWfrMVgcfaqu9Rj9_1a_Ao8flds</recordid><startdate>19950626</startdate><enddate>19950626</enddate><creator>Corkins, M.R.</creator><creator>Vanderhoof, J.A.</creator><creator>Slentz, D.H.</creator><creator>Macdonald, R.G.</creator><creator>Park, J.H.Y.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19950626</creationdate><title>Growth Stimulation by Transfection of Intestinal Epithelial Cells with an Antisense Insulin-like Growth Factor-Binding Protein-2 Construct</title><author>Corkins, M.R. ; Vanderhoof, J.A. ; Slentz, D.H. ; Macdonald, R.G. ; Park, J.H.Y.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c339t-268973393e4bbff5f2ce07e3323ba829cac890106cfb2f432d5508ecf20d4a723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animals</topic><topic>Carrier Proteins - genetics</topic><topic>Carrier Proteins - secretion</topic><topic>Cell Division - drug effects</topic><topic>Cells, Cultured</topic><topic>DNA, Antisense - pharmacology</topic><topic>Epithelial Cells</topic><topic>Epithelium - drug effects</topic><topic>Epithelium - growth & development</topic><topic>Insulin-Like Growth Factor Binding Protein 2</topic><topic>Insulin-Like Growth Factor II - metabolism</topic><topic>Intestines - cytology</topic><topic>Intestines - drug effects</topic><topic>Intestines - growth & development</topic><topic>Rats</topic><topic>RNA, Messenger - analysis</topic><topic>Somatomedins - genetics</topic><topic>Somatomedins - secretion</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Corkins, M.R.</creatorcontrib><creatorcontrib>Vanderhoof, J.A.</creatorcontrib><creatorcontrib>Slentz, D.H.</creatorcontrib><creatorcontrib>Macdonald, R.G.</creatorcontrib><creatorcontrib>Park, J.H.Y.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Corkins, M.R.</au><au>Vanderhoof, J.A.</au><au>Slentz, D.H.</au><au>Macdonald, R.G.</au><au>Park, J.H.Y.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Growth Stimulation by Transfection of Intestinal Epithelial Cells with an Antisense Insulin-like Growth Factor-Binding Protein-2 Construct</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1995-06-26</date><risdate>1995</risdate><volume>211</volume><issue>3</issue><spage>707</spage><epage>713</epage><pages>707-713</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>IEC-6 cells, an intestinal epithelial cell line, produce insulin-like growth factor (IGF)-II and IGF-binding protein-2 (IGFBP-2). Exogenous IGFs stimulate and IGFBP-2 attenuates DNA synthesis. To investigate whether endogenously secreted IGFBP-2 inhibits proliferation, IEC-6 cells were transfected with a full-length rat IGFBP-2 cDNA antisense expression construct. The steady-state level of IGFBP-2 mRNA decreased by 54% in the antisense cDNA-transfected cells (denoted revBP2) compared with vector-transfected controls. Moreover, revBP2 cells secreted less IGFBP-2 than controls (maximally a 68% decrease). In serum-containing medium, revBP2 cells exhibited a 35% increase in log-phase proliferation rate and growth-arrested at a maximum density 14% higher than controls. We conclude that endogenous IGFBP-2 inhibits proliferation of IEC-6 cells, probably by sequestering IGFs.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>7541197</pmid><doi>10.1006/bbrc.1995.1870</doi><tpages>7</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1995-06, Vol.211 (3), p.707-713 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Carrier Proteins - genetics Carrier Proteins - secretion Cell Division - drug effects Cells, Cultured DNA, Antisense - pharmacology Epithelial Cells Epithelium - drug effects Epithelium - growth & development Insulin-Like Growth Factor Binding Protein 2 Insulin-Like Growth Factor II - metabolism Intestines - cytology Intestines - drug effects Intestines - growth & development Rats RNA, Messenger - analysis Somatomedins - genetics Somatomedins - secretion Transfection |
| title | Growth Stimulation by Transfection of Intestinal Epithelial Cells with an Antisense Insulin-like Growth Factor-Binding Protein-2 Construct |
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