Immunochemical characterization of protein kinase C in rat liver nuclei and subnuclear fractions
A doublet of immunoreactive bands has been identified in rat liver nuclei, nuclear matrix and lamina by means of a polyclonal antibody against protein kinase C. The two polypeptides show an apparent molecular weight of 77 and 74 kDa on SDS-polyacrylamide gels, and appear to be tightly bound nuclear...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1987-01, Vol.142 (2), p.367-375 |
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| container_end_page | 375 |
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| container_issue | 2 |
| container_start_page | 367 |
| container_title | Biochemical and biophysical research communications |
| container_volume | 142 |
| creator | Capitani, Silvano Girard, Peggy R. Mazzei, Gonzalo J. Kuo, J.F. Berezney, Ronald Manzoli, Francesco A. |
| description | A doublet of immunoreactive bands has been identified in rat liver nuclei, nuclear matrix and lamina by means of a polyclonal antibody against protein kinase C. The two polypeptides show an apparent molecular weight of 77 and 74 kDa on SDS-polyacrylamide gels, and appear to be tightly bound nuclear components, resistant to detergent and high salt extraction. Given the complexity of the genes encoding for protein kinase C, these two forms of the enzyme might be translational products specifically located in the nucleus, involved in the transduction to the genomic apparatus of regulatory signals generated by growth factors and tumor promoters. |
| doi_str_mv | 10.1016/0006-291X(87)90283-X |
| format | Article |
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The two polypeptides show an apparent molecular weight of 77 and 74 kDa on SDS-polyacrylamide gels, and appear to be tightly bound nuclear components, resistant to detergent and high salt extraction. Given the complexity of the genes encoding for protein kinase C, these two forms of the enzyme might be translational products specifically located in the nucleus, involved in the transduction to the genomic apparatus of regulatory signals generated by growth factors and tumor promoters.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/0006-291X(87)90283-X</identifier><identifier>PMID: 3814141</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Cell Nucleus - enzymology ; Lipids - pharmacology ; Liver - enzymology ; Male ; Protein Kinase C - analysis ; Protein Kinase C - immunology ; Protein Kinase C - physiology ; Rats ; Rats, Inbred Strains</subject><ispartof>Biochemical and biophysical research communications, 1987-01, Vol.142 (2), p.367-375</ispartof><rights>1987</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-96f72aa28f254c63ead1510017d881bfa7211e9968ffca94da586deca9ffa5003</citedby><cites>FETCH-LOGICAL-c357t-96f72aa28f254c63ead1510017d881bfa7211e9968ffca94da586deca9ffa5003</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0006-291X(87)90283-X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27926,27927,45997</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3814141$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Capitani, Silvano</creatorcontrib><creatorcontrib>Girard, Peggy R.</creatorcontrib><creatorcontrib>Mazzei, Gonzalo J.</creatorcontrib><creatorcontrib>Kuo, J.F.</creatorcontrib><creatorcontrib>Berezney, Ronald</creatorcontrib><creatorcontrib>Manzoli, Francesco A.</creatorcontrib><title>Immunochemical characterization of protein kinase C in rat liver nuclei and subnuclear fractions</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>A doublet of immunoreactive bands has been identified in rat liver nuclei, nuclear matrix and lamina by means of a polyclonal antibody against protein kinase C. The two polypeptides show an apparent molecular weight of 77 and 74 kDa on SDS-polyacrylamide gels, and appear to be tightly bound nuclear components, resistant to detergent and high salt extraction. Given the complexity of the genes encoding for protein kinase C, these two forms of the enzyme might be translational products specifically located in the nucleus, involved in the transduction to the genomic apparatus of regulatory signals generated by growth factors and tumor promoters.</description><subject>Animals</subject><subject>Cell Nucleus - enzymology</subject><subject>Lipids - pharmacology</subject><subject>Liver - enzymology</subject><subject>Male</subject><subject>Protein Kinase C - analysis</subject><subject>Protein Kinase C - immunology</subject><subject>Protein Kinase C - physiology</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtLAzEUhYMoWqv_QCEr0cVoMq8kG0GKLyi4Uegu3mZuMDoPTWYK-uvN2NKlZJEb7jnn3nyEnHB2yRkvrxhjZZIqvjiX4kKxVGbJYodMOFMsSTnLd8lkKzkghyG8M8Z5Xqp9sp9JnsczIa-PTTO0nXnDxhmoqXkDD6ZH736gd11LO0s_fdeja-mHayEgndFYe-hp7VboaTuYGh2FtqJhWP69wFM7pkR_OCJ7FuqAx5t7Sl7ubp9nD8n86f5xdjNPTFaIPlGlFSlAKm1a5KbMECpe8LiwqKTkSwsi5RyVKqW1BlReQSHLCmNpLRSMZVNyts6N234NGHrduGCwrqHFbghaiEylIldRmK-FxncheLT607sG_LfmTI9g9UhNj9S0FPoPrF5E2-kmf1g2WG1NG5Kxf73uY_zkyqHXwThsDVbOo-l11bn_B_wCpZCJxg</recordid><startdate>19870130</startdate><enddate>19870130</enddate><creator>Capitani, Silvano</creator><creator>Girard, Peggy R.</creator><creator>Mazzei, Gonzalo J.</creator><creator>Kuo, J.F.</creator><creator>Berezney, Ronald</creator><creator>Manzoli, Francesco A.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19870130</creationdate><title>Immunochemical characterization of protein kinase C in rat liver nuclei and subnuclear fractions</title><author>Capitani, Silvano ; Girard, Peggy R. ; Mazzei, Gonzalo J. ; Kuo, J.F. ; Berezney, Ronald ; Manzoli, Francesco A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-96f72aa28f254c63ead1510017d881bfa7211e9968ffca94da586deca9ffa5003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Animals</topic><topic>Cell Nucleus - enzymology</topic><topic>Lipids - pharmacology</topic><topic>Liver - enzymology</topic><topic>Male</topic><topic>Protein Kinase C - analysis</topic><topic>Protein Kinase C - immunology</topic><topic>Protein Kinase C - physiology</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Capitani, Silvano</creatorcontrib><creatorcontrib>Girard, Peggy R.</creatorcontrib><creatorcontrib>Mazzei, Gonzalo J.</creatorcontrib><creatorcontrib>Kuo, J.F.</creatorcontrib><creatorcontrib>Berezney, Ronald</creatorcontrib><creatorcontrib>Manzoli, Francesco A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Capitani, Silvano</au><au>Girard, Peggy R.</au><au>Mazzei, Gonzalo J.</au><au>Kuo, J.F.</au><au>Berezney, Ronald</au><au>Manzoli, Francesco A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunochemical characterization of protein kinase C in rat liver nuclei and subnuclear fractions</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1987-01-30</date><risdate>1987</risdate><volume>142</volume><issue>2</issue><spage>367</spage><epage>375</epage><pages>367-375</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>A doublet of immunoreactive bands has been identified in rat liver nuclei, nuclear matrix and lamina by means of a polyclonal antibody against protein kinase C. The two polypeptides show an apparent molecular weight of 77 and 74 kDa on SDS-polyacrylamide gels, and appear to be tightly bound nuclear components, resistant to detergent and high salt extraction. Given the complexity of the genes encoding for protein kinase C, these two forms of the enzyme might be translational products specifically located in the nucleus, involved in the transduction to the genomic apparatus of regulatory signals generated by growth factors and tumor promoters.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>3814141</pmid><doi>10.1016/0006-291X(87)90283-X</doi><tpages>9</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1987-01, Vol.142 (2), p.367-375 |
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| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Cell Nucleus - enzymology Lipids - pharmacology Liver - enzymology Male Protein Kinase C - analysis Protein Kinase C - immunology Protein Kinase C - physiology Rats Rats, Inbred Strains |
| title | Immunochemical characterization of protein kinase C in rat liver nuclei and subnuclear fractions |
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