The use of neuronal networks on multielectrode arrays as biosensors
Mammalian spinal neuronal networks growing on arrays of photo-etched electrodes in culture provide a highly stable system for the long-term monitoring of multichannel, spontaneous or evoked electrophysiological activity. In the absence of the homeostatic control mechanisms of the central nervous sys...
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| Veröffentlicht in: | Biosensors & bioelectronics 1995, Vol.10 (6), p.553-567 |
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| container_title | Biosensors & bioelectronics |
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| creator | Gross, Guenter W. Rhoades, Barry K. Azzazy, Hassan M.E. Ming-Chi Wu |
| description | Mammalian spinal neuronal networks growing on arrays of photo-etched electrodes in culture provide a highly stable system for the long-term monitoring of multichannel, spontaneous or evoked electrophysiological activity. In the absence of the homeostatic control mechanisms of the central nervous system, these networks show remarkable sensitivities to minute chemical changes and mimic some of the properties of sensory tissue. These sensitivities could be enhanced by receptor up-regulation and altered by the expression of unique receptors. The fault-tolerant spontaneous network activity is used as a dynamic platform on which large changes in activity signify detection of chemical substances. We present strategies for the expression of novel supersensitivities to foreign molecules via genetic engineering that involves the grafting of ligand binding cDNA onto truncated native receptor DNA and the subsequent expression of such chimeric receptors. |
| doi_str_mv | 10.1016/0956-5663(95)96931-N |
| format | Article |
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In the absence of the homeostatic control mechanisms of the central nervous system, these networks show remarkable sensitivities to minute chemical changes and mimic some of the properties of sensory tissue. These sensitivities could be enhanced by receptor up-regulation and altered by the expression of unique receptors. The fault-tolerant spontaneous network activity is used as a dynamic platform on which large changes in activity signify detection of chemical substances. We present strategies for the expression of novel supersensitivities to foreign molecules via genetic engineering that involves the grafting of ligand binding cDNA onto truncated native receptor DNA and the subsequent expression of such chimeric receptors.</description><subject>Animals</subject><subject>Automatic Data Processing</subject><subject>Biological and medical sciences</subject><subject>Biosensing Techniques</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Central nervous system</subject><subject>chimeric receptors</subject><subject>Electrophysiology</subject><subject>extracellular recording</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gamma-Aminobutyric Acid - pharmacology</subject><subject>Glycine - pharmacology</subject><subject>liposomes</subject><subject>Methods. Procedures. Technologies</subject><subject>Mice</subject><subject>N-Methylaspartate - pharmacology</subject><subject>nerve cell biosensors</subject><subject>Nerve Net - physiology</subject><subject>Neurons - drug effects</subject><subject>Neurons - physiology</subject><subject>sensomimes</subject><subject>Transfection</subject><subject>Various methods and equipments</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU9L5EAQxRtx0fHPN1DIQUQP2e1Kp7vTF0GGVRfEvei56aQr2JpJa1ei-O034wxz1D1VwftV8XiPsSPgP4GD-sWNVLlUSpwZeW6UEZDfbbEZVFrkZSHkNpttkF22R_TEOddg-A7b0QqKgusZm98_YjYSZrHNehxT7F03LcN7TM-UxT5bjN0QsMNmSNFj5lJyH5Q5yuoQCXuKiQ7Yj9Z1hIfruc8ern7fz2_y27_Xf-aXt3kjJR9y4LrUqKQCjb70aOoavKulUZX3RVlxWUymQNReALZGOYWikE4VvJZOSiH22enq70uKryPSYBeBGuw612McyWotqkob_i0IyuiyhPJ_QAUg9ASWK7BJkShha19SWLj0YYHbZRt2GbVdRm2NtJ9t2Lvp7Hj9f6wX6DdH6_gn_WStO2pc1ybXN4E2mJCGy2qJXawwnNJ9C5gsNQH7Bn1IUzHWx_C1j38DdqTh</recordid><startdate>1995</startdate><enddate>1995</enddate><creator>Gross, Guenter W.</creator><creator>Rhoades, Barry K.</creator><creator>Azzazy, Hassan M.E.</creator><creator>Ming-Chi Wu</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>1995</creationdate><title>The use of neuronal networks on multielectrode arrays as biosensors</title><author>Gross, Guenter W. ; Rhoades, Barry K. ; Azzazy, Hassan M.E. ; Ming-Chi Wu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c550t-10747e65617ed4de9bb1dab5968dd24805212213bd31ef96a6e325a620b5a5533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animals</topic><topic>Automatic Data Processing</topic><topic>Biological and medical sciences</topic><topic>Biosensing Techniques</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Central nervous system</topic><topic>chimeric receptors</topic><topic>Electrophysiology</topic><topic>extracellular recording</topic><topic>Fundamental and applied biological sciences. 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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Automatic Data Processing Biological and medical sciences Biosensing Techniques Biosensors Biotechnology Central nervous system chimeric receptors Electrophysiology extracellular recording Fundamental and applied biological sciences. Psychology gamma-Aminobutyric Acid - pharmacology Glycine - pharmacology liposomes Methods. Procedures. Technologies Mice N-Methylaspartate - pharmacology nerve cell biosensors Nerve Net - physiology Neurons - drug effects Neurons - physiology sensomimes Transfection Various methods and equipments Vertebrates: nervous system and sense organs |
| title | The use of neuronal networks on multielectrode arrays as biosensors |
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