Strain variation in the circumsporozoite protein gene of Plasmodium falciparum
The complete nucleotide sequence of the cloned circumsporozoite protein gene of the Plasmodium falciparum Wellcome (West African) isolate has been determined. The sequence shows two striking differences from that of the published Brazilian strain; the total number of tandem 12 base pair repeats is 4...
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| Veröffentlicht in: | Molecular and biochemical parasitology 1987-01, Vol.22 (1), p.101-108 |
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| container_title | Molecular and biochemical parasitology |
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| creator | Lockyer, Michael J. Schwarz, Ralph T. |
| description | The complete nucleotide sequence of the cloned circumsporozoite protein gene of the
Plasmodium falciparum Wellcome (West African) isolate has been determined. The sequence shows two striking differences from that of the published Brazilian strain; the total number of tandem 12 base pair repeats is 46 compared to 41, and the 5′ coding region contains an additional 30 nucleotides. From Southern blot experiments, two out of four cloned Thai lines also have a similar, higher number of repeats. Heterogeneity in the CSP gene repeat region and in the length of the 5′ coding region allows the strains to be classed into three groups, with the Wellcome strain being indistinguishable from the Thai line T9-94. |
| doi_str_mv | 10.1016/0166-6851(87)90073-9 |
| format | Article |
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Plasmodium falciparum Wellcome (West African) isolate has been determined. The sequence shows two striking differences from that of the published Brazilian strain; the total number of tandem 12 base pair repeats is 46 compared to 41, and the 5′ coding region contains an additional 30 nucleotides. From Southern blot experiments, two out of four cloned Thai lines also have a similar, higher number of repeats. Heterogeneity in the CSP gene repeat region and in the length of the 5′ coding region allows the strains to be classed into three groups, with the Wellcome strain being indistinguishable from the Thai line T9-94.</description><subject>5′ Coding region</subject><subject>Animals</subject><subject>Antigens, Protozoan - genetics</subject><subject>Antigens, Surface - genetics</subject><subject>Biological and medical sciences</subject><subject>Circumsporozoite protein gene</subject><subject>Cloning, Molecular</subject><subject>DNA - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>Genes. 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Plasmodium falciparum Wellcome (West African) isolate has been determined. The sequence shows two striking differences from that of the published Brazilian strain; the total number of tandem 12 base pair repeats is 46 compared to 41, and the 5′ coding region contains an additional 30 nucleotides. From Southern blot experiments, two out of four cloned Thai lines also have a similar, higher number of repeats. Heterogeneity in the CSP gene repeat region and in the length of the 5′ coding region allows the strains to be classed into three groups, with the Wellcome strain being indistinguishable from the Thai line T9-94.</abstract><cop>Shannon</cop><pub>Elsevier B.V</pub><pmid>3543671</pmid><doi>10.1016/0166-6851(87)90073-9</doi><tpages>8</tpages></addata></record> |
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| ispartof | Molecular and biochemical parasitology, 1987-01, Vol.22 (1), p.101-108 |
| issn | 0166-6851 1872-9428 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | 5′ Coding region Animals Antigens, Protozoan - genetics Antigens, Surface - genetics Biological and medical sciences Circumsporozoite protein gene Cloning, Molecular DNA - analysis Fundamental and applied biological sciences. Psychology Genes Genes. Genome Human protozoal diseases Infectious diseases Malaria Medical sciences Molecular and cellular biology Molecular genetics Nucleic Acid Hybridization Parasitic diseases Plasmodium falciparum Plasmodium falciparum - genetics Plasmodium falciparum - immunology Protozoal diseases Protozoan Proteins Repeat region Repetitive Sequences, Nucleic Acid Sequence Homology, Nucleic Acid Tropical medicine |
| title | Strain variation in the circumsporozoite protein gene of Plasmodium falciparum |
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