Analysis of the structural integrity of YACs comprising human immunoglobulin genes in yeast and in embryonic stem cells

With the goal of creating a strain of mice capable of producing human antibodies, we are cloning and reconstructing the human immunoglobulin germline repertoire in yeast artificial chromosomes (YACs). We describe the identification of YACs containing variable and constant region sequences from the h...

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Veröffentlicht in:	Genomics 1995-03, Vol.26 (2), p.294-307
Hauptverfasser:	Mendez, Michael J., Abderrahim, Hadi, Noguchi, Masato, David, Nathaniel E., Hardy, Margaret C., Green, Larry L., Tsuda, Hirohisa, Yoast, Sienna, Maynard-Currie, Catherine E., Garza, Dan, Gemmill, Robert, Jakobovits, Aya, Klapholz, Sue
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals ANTIBODIES B-Lymphocytes Base Sequence Biological and medical sciences BIOLOGY AND MEDICINE, BASIC STUDIES Cell Fusion Chromosomes, Artificial, Yeast Cloning, Molecular DNA HYBRIDIZATION DNA SEQUENCING DNA, Recombinant - genetics DNA-CLONING Embryo, Mammalian - cytology EMBRYONIC CELLS Fibroblasts Fundamental and applied biological sciences. Psychology Gene Library GENE MUTATIONS GENES Genes, Immunoglobulin Genes. Genome Humans Hypoxanthine Phosphoribosyltransferase - deficiency Hypoxanthine Phosphoribosyltransferase - genetics Immunoglobulin Constant Regions - genetics Immunoglobulin Heavy Chains - genetics Immunoglobulin J-Chains - genetics Immunoglobulin kappa-Chains - genetics Immunoglobulin Variable Region - genetics IMMUNOGLOBULINS INSTABILITY MICE Molecular and cellular biology Molecular genetics Molecular Sequence Data POLYMERASE CHAIN REACTION Saccharomyces cerevisiae - genetics Selection, Genetic Stem Cells YEASTS
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