Methods for the Detection of a Specific Mycobacterium leprae Antigen in the Urine of Leprosy Patients
Two methods for detecting the phenolic glycolipid, PGL‐1, a Mycobacterium leprae‐specific molecule, in the urine of leprosy patients are described. Both methods rely on the 100‐fold preconcentration of the urine, which can be accomplished by a single‐step ultrafiltration procedure. The equivalent of...
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| Veröffentlicht in: | Scandinavian journal of immunology 1987-01, Vol.25 (1), p.37-43 |
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| container_end_page | 43 |
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| container_start_page | 37 |
| container_title | Scandinavian journal of immunology |
| container_volume | 25 |
| creator | KALDANY, RASHAD‐RUDOLF J. MAASHO, KERIMA OHMAN, R. REITZ‐VICK, D. BRITTON, S. LEFFORD, M. J. |
| description | Two methods for detecting the phenolic glycolipid, PGL‐1, a Mycobacterium leprae‐specific molecule, in the urine of leprosy patients are described. Both methods rely on the 100‐fold preconcentration of the urine, which can be accomplished by a single‐step ultrafiltration procedure. The equivalent of approximately 2.5/μ of PGL‐1/ml was detected in the urine of LL patients with an inhibition ELISA. The second method, a direct dot‐blot assay on nitrocellulose paper, was much simpler and more sensitive. As little as 3 ng of antigen was detected by the dot‐bolt technique. PGL‐1 was detected in the urine of LL patients. |
| doi_str_mv | 10.1111/j.1365-3083.1987.tb01044.x |
| format | Article |
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PGL‐1 was detected in the urine of LL patients.</description><subject>Antigens, Bacterial - urine</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Glycolipids - urine</subject><subject>Humans</subject><subject>Immunoelectrophoresis</subject><subject>Leprosy - immunology</subject><subject>Leprosy - pathology</subject><subject>Leprosy - urine</subject><subject>Mycobacterium leprae - immunology</subject><issn>0300-9475</issn><issn>1365-3083</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkE1PwzAMhiMEgvHxE5AiDtxakrpZUiQO0_jWEEiwc5SmLsvUtaPJBPv3tGzaHV98eP3Y1kPIBWcx7-pqHnMYigiYgphnSsYhZ5ylafyzRwa7aJ8MGDAWZakUR-TY-zljHBIJh-QQRJp25IDgC4ZZU3haNi0NM6S3GNAG19S0Kamh70u0rnSWvqxtkxsbsHWrBa1w2Rqkozq4T6ypq__Yaetq7LlJFzd-Td9McFgHf0oOSlN5PNv2EzK9v_sYP0aT14en8WgSWRgqGRVFptAAy1BmLCnylHGRCit5rkwixBBAlFmJIsuNkkIWCSgQKIFhOlSAGZyQy83e7vzXCn3QC-ctVpWpsVl5LSUophLWDV5vBm33p2-x1MvWLUy71pzp3rGe616k7kXq3rHeOtY_HXy-vbLKF1js0K3ULr_Z5N-uwvU_Nuv35yeQ8AvH-YtI</recordid><startdate>198701</startdate><enddate>198701</enddate><creator>KALDANY, RASHAD‐RUDOLF J.</creator><creator>MAASHO, KERIMA</creator><creator>OHMAN, R.</creator><creator>REITZ‐VICK, D.</creator><creator>BRITTON, S.</creator><creator>LEFFORD, M. 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J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Scandinavian journal of immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>KALDANY, RASHAD‐RUDOLF J.</au><au>MAASHO, KERIMA</au><au>OHMAN, R.</au><au>REITZ‐VICK, D.</au><au>BRITTON, S.</au><au>LEFFORD, M. J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Methods for the Detection of a Specific Mycobacterium leprae Antigen in the Urine of Leprosy Patients</atitle><jtitle>Scandinavian journal of immunology</jtitle><addtitle>Scand J Immunol</addtitle><date>1987-01</date><risdate>1987</risdate><volume>25</volume><issue>1</issue><spage>37</spage><epage>43</epage><pages>37-43</pages><issn>0300-9475</issn><eissn>1365-3083</eissn><abstract>Two methods for detecting the phenolic glycolipid, PGL‐1, a Mycobacterium leprae‐specific molecule, in the urine of leprosy patients are described. Both methods rely on the 100‐fold preconcentration of the urine, which can be accomplished by a single‐step ultrafiltration procedure. The equivalent of approximately 2.5/μ of PGL‐1/ml was detected in the urine of LL patients with an inhibition ELISA. The second method, a direct dot‐blot assay on nitrocellulose paper, was much simpler and more sensitive. As little as 3 ng of antigen was detected by the dot‐bolt technique. PGL‐1 was detected in the urine of LL patients.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>3544198</pmid><doi>10.1111/j.1365-3083.1987.tb01044.x</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0300-9475 |
| ispartof | Scandinavian journal of immunology, 1987-01, Vol.25 (1), p.37-43 |
| issn | 0300-9475 1365-3083 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77380820 |
| source | Wiley-Blackwell Journals; MEDLINE |
| subjects | Antigens, Bacterial - urine Enzyme-Linked Immunosorbent Assay Glycolipids - urine Humans Immunoelectrophoresis Leprosy - immunology Leprosy - pathology Leprosy - urine Mycobacterium leprae - immunology |
| title | Methods for the Detection of a Specific Mycobacterium leprae Antigen in the Urine of Leprosy Patients |
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