Reference ranges for hemoglobin variants by HPLC in african americans
High performance liquid chromatography (HPLC) demonstrated advantages over conventional procedures employed in newborn and adult hemoglobinopathy screening programs for the identification of Hb variants has promoted the need to reassess our knowledge of hemoglobin reference ranges as it relates to H...
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| Veröffentlicht in: | Annals of clinical and laboratory science 1995-05, Vol.25 (3), p.228-235 |
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| creator | ROA, D TURNER, E. A DEL PILAR AGUINAGA, M |
| description | High performance liquid chromatography (HPLC) demonstrated advantages over conventional procedures employed in newborn and adult hemoglobinopathy screening programs for the identification of Hb variants has promoted the need to reassess our knowledge of hemoglobin reference ranges as it relates to HPLC quantitation. In this study, the HPLC hemoglobin reference ranges derived from 200 normal African American adults are expressed as follows: Hb A mean 93.6 percent (s.d. 1.3, ranges 89.8 to 95.2), Hb A1 mean 2.0 percent (s.d. 0.6, ranges 0.8 to 5.2), Hb F mean 3.2 percent (s.d. 0.7, ranges 1.7 to 5.3) and Hb A2 mean 1.2 percent (s.d. 0.4, ranges 0.5 to 3.4); while the HPLC results for normal newborns and babies (n = 99) in the African American population fluctuates from Hb F mean 82.0 percent (s.d. 7.7, range 66.6 to 89.9) and Hb A mean 19.0 percent (s.d. 7.7, ranges 10.1 to 33.4) at 4 days to a mean of 15. percent (s.d. 4.8, range 9.3 to 22.8) for Hb F and a mean of 85.0 percent (s.d. 5.1, ranges 76.4 to 90.7) for Hb A at 300 days after birth. In case of the most common hemoglobin variants for this population, it has been shown that the A/S and A/C ratios for adults (Hb AS, Hb AC) and newborns (Hb FAS, and FAC) remained within the 1.5 (range 1.0 to 2.2) limits regardless of age group. Application of these HPLC ranges to confront other abnormalities will prove most useful during blood screening processes. |
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A ; DEL PILAR AGUINAGA, M</creator><creatorcontrib>ROA, D ; TURNER, E. A ; DEL PILAR AGUINAGA, M</creatorcontrib><description>High performance liquid chromatography (HPLC) demonstrated advantages over conventional procedures employed in newborn and adult hemoglobinopathy screening programs for the identification of Hb variants has promoted the need to reassess our knowledge of hemoglobin reference ranges as it relates to HPLC quantitation. In this study, the HPLC hemoglobin reference ranges derived from 200 normal African American adults are expressed as follows: Hb A mean 93.6 percent (s.d. 1.3, ranges 89.8 to 95.2), Hb A1 mean 2.0 percent (s.d. 0.6, ranges 0.8 to 5.2), Hb F mean 3.2 percent (s.d. 0.7, ranges 1.7 to 5.3) and Hb A2 mean 1.2 percent (s.d. 0.4, ranges 0.5 to 3.4); while the HPLC results for normal newborns and babies (n = 99) in the African American population fluctuates from Hb F mean 82.0 percent (s.d. 7.7, range 66.6 to 89.9) and Hb A mean 19.0 percent (s.d. 7.7, ranges 10.1 to 33.4) at 4 days to a mean of 15. percent (s.d. 4.8, range 9.3 to 22.8) for Hb F and a mean of 85.0 percent (s.d. 5.1, ranges 76.4 to 90.7) for Hb A at 300 days after birth. In case of the most common hemoglobin variants for this population, it has been shown that the A/S and A/C ratios for adults (Hb AS, Hb AC) and newborns (Hb FAS, and FAC) remained within the 1.5 (range 1.0 to 2.2) limits regardless of age group. Application of these HPLC ranges to confront other abnormalities will prove most useful during blood screening processes.</description><identifier>ISSN: 0091-7370</identifier><identifier>EISSN: 1550-8080</identifier><identifier>PMID: 7605104</identifier><identifier>CODEN: ACLSCP</identifier><language>eng</language><publisher>Philadelphia, PA: Institute for Clinical Science</publisher><subject>Adolescent ; Adult ; Age Factors ; Biological and medical sciences ; Black or African American ; Black People ; Chromatography, High Pressure Liquid ; Female ; Fundamental and applied biological sciences. Psychology ; Hemoglobinopathies ; Hemoglobins - analysis ; Hemoglobins - classification ; Hemoglobins, Abnormal - analysis ; Humans ; Infant ; Infant, Newborn - blood ; Male ; Middle Aged ; Reference Values ; Sickle Cell Trait - blood ; Vertebrates: blood, hematopoietic organs, reticuloendothelial system</subject><ispartof>Annals of clinical and laboratory science, 1995-05, Vol.25 (3), p.228-235</ispartof><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3535685$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7605104$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>ROA, D</creatorcontrib><creatorcontrib>TURNER, E. A</creatorcontrib><creatorcontrib>DEL PILAR AGUINAGA, M</creatorcontrib><title>Reference ranges for hemoglobin variants by HPLC in african americans</title><title>Annals of clinical and laboratory science</title><addtitle>Ann Clin Lab Sci</addtitle><description>High performance liquid chromatography (HPLC) demonstrated advantages over conventional procedures employed in newborn and adult hemoglobinopathy screening programs for the identification of Hb variants has promoted the need to reassess our knowledge of hemoglobin reference ranges as it relates to HPLC quantitation. In this study, the HPLC hemoglobin reference ranges derived from 200 normal African American adults are expressed as follows: Hb A mean 93.6 percent (s.d. 1.3, ranges 89.8 to 95.2), Hb A1 mean 2.0 percent (s.d. 0.6, ranges 0.8 to 5.2), Hb F mean 3.2 percent (s.d. 0.7, ranges 1.7 to 5.3) and Hb A2 mean 1.2 percent (s.d. 0.4, ranges 0.5 to 3.4); while the HPLC results for normal newborns and babies (n = 99) in the African American population fluctuates from Hb F mean 82.0 percent (s.d. 7.7, range 66.6 to 89.9) and Hb A mean 19.0 percent (s.d. 7.7, ranges 10.1 to 33.4) at 4 days to a mean of 15. percent (s.d. 4.8, range 9.3 to 22.8) for Hb F and a mean of 85.0 percent (s.d. 5.1, ranges 76.4 to 90.7) for Hb A at 300 days after birth. In case of the most common hemoglobin variants for this population, it has been shown that the A/S and A/C ratios for adults (Hb AS, Hb AC) and newborns (Hb FAS, and FAC) remained within the 1.5 (range 1.0 to 2.2) limits regardless of age group. Application of these HPLC ranges to confront other abnormalities will prove most useful during blood screening processes.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Age Factors</subject><subject>Biological and medical sciences</subject><subject>Black or African American</subject><subject>Black People</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hemoglobinopathies</subject><subject>Hemoglobins - analysis</subject><subject>Hemoglobins - classification</subject><subject>Hemoglobins, Abnormal - analysis</subject><subject>Humans</subject><subject>Infant</subject><subject>Infant, Newborn - blood</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Reference Values</subject><subject>Sickle Cell Trait - blood</subject><subject>Vertebrates: blood, hematopoietic organs, reticuloendothelial system</subject><issn>0091-7370</issn><issn>1550-8080</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9j1tLw0AUhBdRaq3-BGEfxLfASfb-KKVaoaCIPofN5pw2kkvdbYX-e4MGn2aY-RiYMzbPlYLMgoVzNgdweWaEgUt2ldInQOGkhBmbGQ0qBzlnqzckjNgH5NH3W0ychsh32A3bdqiann_72Pj-kHh14uvXzZKPmafYBD9qh78mXbML8m3Cm0kX7ONx9b5cZ5uXp-flwybbFVodssoWwgnShiwKa51TJAhDXYAuyBn0UiuqQy4QUTlXSSNrLYkKtFpiXYkFu__b3cfh64jpUHZNCti2vsfhmEpjhAVp7QjeTuCx6rAu97HpfDyV0--xv5t6n4JvafwemvSPCSWUtkr8AKKFYWI</recordid><startdate>19950501</startdate><enddate>19950501</enddate><creator>ROA, D</creator><creator>TURNER, E. A</creator><creator>DEL PILAR AGUINAGA, M</creator><general>Institute for Clinical Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19950501</creationdate><title>Reference ranges for hemoglobin variants by HPLC in african americans</title><author>ROA, D ; TURNER, E. A ; DEL PILAR AGUINAGA, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h265t-b82393f67f8e388995f3fecd2062f97ea465fdc13eee599b474d64ff2e864edb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Age Factors</topic><topic>Biological and medical sciences</topic><topic>Black or African American</topic><topic>Black People</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hemoglobinopathies</topic><topic>Hemoglobins - analysis</topic><topic>Hemoglobins - classification</topic><topic>Hemoglobins, Abnormal - analysis</topic><topic>Humans</topic><topic>Infant</topic><topic>Infant, Newborn - blood</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Reference Values</topic><topic>Sickle Cell Trait - blood</topic><topic>Vertebrates: blood, hematopoietic organs, reticuloendothelial system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ROA, D</creatorcontrib><creatorcontrib>TURNER, E. A</creatorcontrib><creatorcontrib>DEL PILAR AGUINAGA, M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Annals of clinical and laboratory science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ROA, D</au><au>TURNER, E. A</au><au>DEL PILAR AGUINAGA, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Reference ranges for hemoglobin variants by HPLC in african americans</atitle><jtitle>Annals of clinical and laboratory science</jtitle><addtitle>Ann Clin Lab Sci</addtitle><date>1995-05-01</date><risdate>1995</risdate><volume>25</volume><issue>3</issue><spage>228</spage><epage>235</epage><pages>228-235</pages><issn>0091-7370</issn><eissn>1550-8080</eissn><coden>ACLSCP</coden><abstract>High performance liquid chromatography (HPLC) demonstrated advantages over conventional procedures employed in newborn and adult hemoglobinopathy screening programs for the identification of Hb variants has promoted the need to reassess our knowledge of hemoglobin reference ranges as it relates to HPLC quantitation. In this study, the HPLC hemoglobin reference ranges derived from 200 normal African American adults are expressed as follows: Hb A mean 93.6 percent (s.d. 1.3, ranges 89.8 to 95.2), Hb A1 mean 2.0 percent (s.d. 0.6, ranges 0.8 to 5.2), Hb F mean 3.2 percent (s.d. 0.7, ranges 1.7 to 5.3) and Hb A2 mean 1.2 percent (s.d. 0.4, ranges 0.5 to 3.4); while the HPLC results for normal newborns and babies (n = 99) in the African American population fluctuates from Hb F mean 82.0 percent (s.d. 7.7, range 66.6 to 89.9) and Hb A mean 19.0 percent (s.d. 7.7, ranges 10.1 to 33.4) at 4 days to a mean of 15. percent (s.d. 4.8, range 9.3 to 22.8) for Hb F and a mean of 85.0 percent (s.d. 5.1, ranges 76.4 to 90.7) for Hb A at 300 days after birth. In case of the most common hemoglobin variants for this population, it has been shown that the A/S and A/C ratios for adults (Hb AS, Hb AC) and newborns (Hb FAS, and FAC) remained within the 1.5 (range 1.0 to 2.2) limits regardless of age group. Application of these HPLC ranges to confront other abnormalities will prove most useful during blood screening processes.</abstract><cop>Philadelphia, PA</cop><pub>Institute for Clinical Science</pub><pmid>7605104</pmid><tpages>8</tpages></addata></record> |
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| subjects | Adolescent Adult Age Factors Biological and medical sciences Black or African American Black People Chromatography, High Pressure Liquid Female Fundamental and applied biological sciences. Psychology Hemoglobinopathies Hemoglobins - analysis Hemoglobins - classification Hemoglobins, Abnormal - analysis Humans Infant Infant, Newborn - blood Male Middle Aged Reference Values Sickle Cell Trait - blood Vertebrates: blood, hematopoietic organs, reticuloendothelial system |
| title | Reference ranges for hemoglobin variants by HPLC in african americans |
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