Sleep-waking changes after c-fos antisense injections in the medial preoptic area
THE preoptic hypothalamus has been consistently implicated in the homeostatic regulation of sleep and waking. Recently, it was shown that periods of either spontaneous or forced wakefulness result in the induction in this region of the immediate-early gene c-fos. In this study, antisense oligonucleo...
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| Veröffentlicht in: | Neuroreport 1995-03, Vol.6 (5), p.801-805 |
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| creator | Cirelli, Chiara Pompeiano, Maria Arrighi, Pieranna Tononi, Giulio |
| description | THE preoptic hypothalamus has been consistently implicated in the homeostatic regulation of sleep and waking. Recently, it was shown that periods of either spontaneous or forced wakefulness result in the induction in this region of the immediate-early gene c-fos. In this study, antisense oligonucleotides complementary to c-fos mRNA were employed to interfere with the expression of Fos protein. Injections in the rat medial preoptic area of c-fos antisense, but not of sense, oligonucleotides blocked the expression of Fos protein detected immunocytochemically. Rats receiving bilateral antisense injections showed a higher percentage of wakefulness the day after the injection than controls receiving sense or sham injections or antisense injections outside the preoptic area. These results suggest that blocking the expression of Fos protein in the preoptic hypothalamus may interfere with the homeostatic regulation of sleep and wakefulness. |
| doi_str_mv | 10.1097/00001756-199503270-00024 |
| format | Article |
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Recently, it was shown that periods of either spontaneous or forced wakefulness result in the induction in this region of the immediate-early gene c-fos. In this study, antisense oligonucleotides complementary to c-fos mRNA were employed to interfere with the expression of Fos protein. Injections in the rat medial preoptic area of c-fos antisense, but not of sense, oligonucleotides blocked the expression of Fos protein detected immunocytochemically. Rats receiving bilateral antisense injections showed a higher percentage of wakefulness the day after the injection than controls receiving sense or sham injections or antisense injections outside the preoptic area. These results suggest that blocking the expression of Fos protein in the preoptic hypothalamus may interfere with the homeostatic regulation of sleep and wakefulness.</description><identifier>ISSN: 0959-4965</identifier><identifier>EISSN: 1473-558X</identifier><identifier>DOI: 10.1097/00001756-199503270-00024</identifier><identifier>PMID: 7605952</identifier><language>eng</language><publisher>Hagerstown, MD: Wolters Kluwer Health | Lippincott Williams & Wilkins</publisher><subject>Animals ; Base Sequence ; Biological and medical sciences ; Fundamental and applied biological sciences. Psychology ; Injections ; Male ; Molecular Sequence Data ; Oligonucleotides, Antisense - pharmacology ; Preoptic Area - drug effects ; Preoptic Area - metabolism ; Proto-Oncogene Proteins c-fos - genetics ; Rats ; Rats, Inbred WKY ; RNA, Complementary - genetics ; RNA, Messenger - genetics ; Sleep - drug effects ; Sleep. 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Recently, it was shown that periods of either spontaneous or forced wakefulness result in the induction in this region of the immediate-early gene c-fos. In this study, antisense oligonucleotides complementary to c-fos mRNA were employed to interfere with the expression of Fos protein. Injections in the rat medial preoptic area of c-fos antisense, but not of sense, oligonucleotides blocked the expression of Fos protein detected immunocytochemically. Rats receiving bilateral antisense injections showed a higher percentage of wakefulness the day after the injection than controls receiving sense or sham injections or antisense injections outside the preoptic area. These results suggest that blocking the expression of Fos protein in the preoptic hypothalamus may interfere with the homeostatic regulation of sleep and wakefulness.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Injections</subject><subject>Male</subject><subject>Molecular Sequence Data</subject><subject>Oligonucleotides, Antisense - pharmacology</subject><subject>Preoptic Area - drug effects</subject><subject>Preoptic Area - metabolism</subject><subject>Proto-Oncogene Proteins c-fos - genetics</subject><subject>Rats</subject><subject>Rats, Inbred WKY</subject><subject>RNA, Complementary - genetics</subject><subject>RNA, Messenger - genetics</subject><subject>Sleep - drug effects</subject><subject>Sleep. Vigilance</subject><subject>Vertebrates: nervous system and sense organs</subject><subject>Wakefulness - drug effects</subject><issn>0959-4965</issn><issn>1473-558X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUuLFDEQgIMo67j6E4QcxFs06Tyqc5TFFyzIsi7sLdTE6p3sZrrbpIfBf2_WGecm1qWK1FcV-IoxruQ7JT28ly0UWCeU91bqDqRoL515wlbKgBbW9rdP2Up664Xxzj5nL2q9b4iXqj9jZ-Ck9bZbsavrTDSLPT6k8Y7HDY53VDkOCxUexTC1elxSpbEST-M9xSVNY20lXzbEt_QjYeZzoWleUuRYCF-yZwPmSq-O-ZzdfPr4_eKLuPz2-evFh0sRjbJGOJJGOg2g47rXYAbnIoJD0OBAdQZNJOpgTUMHBjvoPVolo_XKRlgPXp-zt4e9c5l-7qguYZtqpJxxpGlXAzxu8tD_F1QOdO-UbmB_AGOZai00hLmkLZZfQcnwqD381R5O2sMf7W309fGP3bpJOQ0ePbf-m2Mfa8Q8FBxjqidMG-iNUg0zB2w_5XaB-pB3eyphQ5iXTfjX0fVvDqaYlw</recordid><startdate>19950327</startdate><enddate>19950327</enddate><creator>Cirelli, Chiara</creator><creator>Pompeiano, Maria</creator><creator>Arrighi, Pieranna</creator><creator>Tononi, Giulio</creator><general>Wolters Kluwer Health | Lippincott Williams & Wilkins</general><general>Lippincott Williams and Wilkins</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19950327</creationdate><title>Sleep-waking changes after c-fos antisense injections in the medial preoptic area</title><author>Cirelli, Chiara ; Pompeiano, Maria ; Arrighi, Pieranna ; Tononi, Giulio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4154-6e04063773cb8374f66ca76a73767124a4cee27bef274a2789a510c5915c7bf93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Injections</topic><topic>Male</topic><topic>Molecular Sequence Data</topic><topic>Oligonucleotides, Antisense - pharmacology</topic><topic>Preoptic Area - drug effects</topic><topic>Preoptic Area - metabolism</topic><topic>Proto-Oncogene Proteins c-fos - genetics</topic><topic>Rats</topic><topic>Rats, Inbred WKY</topic><topic>RNA, Complementary - genetics</topic><topic>RNA, Messenger - genetics</topic><topic>Sleep - drug effects</topic><topic>Sleep. Vigilance</topic><topic>Vertebrates: nervous system and sense organs</topic><topic>Wakefulness - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cirelli, Chiara</creatorcontrib><creatorcontrib>Pompeiano, Maria</creatorcontrib><creatorcontrib>Arrighi, Pieranna</creatorcontrib><creatorcontrib>Tononi, Giulio</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroreport</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cirelli, Chiara</au><au>Pompeiano, Maria</au><au>Arrighi, Pieranna</au><au>Tononi, Giulio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sleep-waking changes after c-fos antisense injections in the medial preoptic area</atitle><jtitle>Neuroreport</jtitle><addtitle>Neuroreport</addtitle><date>1995-03-27</date><risdate>1995</risdate><volume>6</volume><issue>5</issue><spage>801</spage><epage>805</epage><pages>801-805</pages><issn>0959-4965</issn><eissn>1473-558X</eissn><abstract>THE preoptic hypothalamus has been consistently implicated in the homeostatic regulation of sleep and waking. Recently, it was shown that periods of either spontaneous or forced wakefulness result in the induction in this region of the immediate-early gene c-fos. In this study, antisense oligonucleotides complementary to c-fos mRNA were employed to interfere with the expression of Fos protein. Injections in the rat medial preoptic area of c-fos antisense, but not of sense, oligonucleotides blocked the expression of Fos protein detected immunocytochemically. Rats receiving bilateral antisense injections showed a higher percentage of wakefulness the day after the injection than controls receiving sense or sham injections or antisense injections outside the preoptic area. These results suggest that blocking the expression of Fos protein in the preoptic hypothalamus may interfere with the homeostatic regulation of sleep and wakefulness.</abstract><cop>Hagerstown, MD</cop><pub>Wolters Kluwer Health | Lippincott Williams & Wilkins</pub><pmid>7605952</pmid><doi>10.1097/00001756-199503270-00024</doi><tpages>5</tpages></addata></record> |
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| ispartof | Neuroreport, 1995-03, Vol.6 (5), p.801-805 |
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| language | eng |
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| source | MEDLINE; Journals@Ovid Ovid Autoload |
| subjects | Animals Base Sequence Biological and medical sciences Fundamental and applied biological sciences. Psychology Injections Male Molecular Sequence Data Oligonucleotides, Antisense - pharmacology Preoptic Area - drug effects Preoptic Area - metabolism Proto-Oncogene Proteins c-fos - genetics Rats Rats, Inbred WKY RNA, Complementary - genetics RNA, Messenger - genetics Sleep - drug effects Sleep. Vigilance Vertebrates: nervous system and sense organs Wakefulness - drug effects |
| title | Sleep-waking changes after c-fos antisense injections in the medial preoptic area |
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