Immunocytochemical characterization of explant cultures of human prostatic stromal cells

Immunocytochemical characterization of explant cultures of human prostatic stromal cells

The study of stromal‐epithelial interactions greatly depends on the ability to culture both cell types separately, in order to permit analysis of their interactions under defined conditions in reconstitution experiments. Here we report the establishment of explant cultures of human prostatic stromal...

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Veröffentlicht in:The Prostate 1995-07, Vol.27 (1), p.42-49
Hauptverfasser: Kooistra, Anko, Elissen, Nancy M. J., König, Josée J., Vermey, Marcel, Van Kwast, Theodorus H. Der, Romijn, Johan C., Schröder, Fritz H.
Format: Artikel
Sprache:eng
Schlagworte:
Acid Phosphatase - analysis
Actins - analysis
benign prostatic hyperplasia
Biological and medical sciences
Biomarkers - analysis
Desmin - analysis
fibroblasts
human prostatic stromal cells
Humans
immunocytochemistry
Immunohistochemistry
intermediate filament proteins
Investigative techniques, diagnostic techniques (general aspects)
Keratins - analysis
Male
Medical sciences
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Prostate
prostatic cancer
Prostatic Hyperplasia - metabolism
Prostatic Hyperplasia - pathology
Prostatic Neoplasms - metabolism
Prostatic Neoplasms - pathology
Receptors, Androgen - analysis
Tumor Cells, Cultured
Urinary system
Vimentin - analysis
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Zusammenfassung:The study of stromal‐epithelial interactions greatly depends on the ability to culture both cell types separately, in order to permit analysis of their interactions under defined conditions in reconstitution experiments. Here we report the establishment of explant cultures of human prostatic stromal cells and their immunocytochemical characterization. As determined by antibodies to keratin and prostate specific acid phosphatase, only small numbers (
ISSN:0270-4137
1097-0045
DOI:10.1002/pros.2990270108