Antigen receptor-triggered secretion of a trypsin-type esterase from cytotoxic T lymphocytes
Exocytosis of cytolysin-containing granules from cytotoxic T lymphocytes (CTL) was studied with the use of granule enzyme (BLT esterase) as a convenient biochemical marker. Using cloned CTL, we demonstrate here that BLT esterase secretion into the supernatant is specifically triggered by antigen-bea...
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| Veröffentlicht in: | The Journal of immunology (1950) 1987-01, Vol.138 (2), p.566-569 |
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| container_title | The Journal of immunology (1950) |
| container_volume | 138 |
| creator | Takayama, H Trenn, G Humphrey, W, Jr Bluestone, JA Henkart, PA Sitkovsky, MV |
| description | Exocytosis of cytolysin-containing granules from cytotoxic T lymphocytes (CTL) was studied with the use of granule enzyme (BLT esterase) as a convenient biochemical marker. Using cloned CTL, we demonstrate here that BLT esterase secretion into the supernatant is specifically triggered by antigen-bearing target cells and that this secretion is inhibited by soluble monoclonal antibodies against the antigen-specific T cell receptor (TcR). Immobilized anti-receptor antibodies induced efficient enzyme secretion in the absence of target cells, thus implying a direct involvement of TcR complex in triggering exocytosis of granules. These results support the role of the granule exocytosis in CTL functions and provide a quantitative and direct assay of a rapid CTL functional response to antigenic stimulation. |
| doi_str_mv | 10.4049/jimmunol.138.2.566 |
| format | Article |
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Using cloned CTL, we demonstrate here that BLT esterase secretion into the supernatant is specifically triggered by antigen-bearing target cells and that this secretion is inhibited by soluble monoclonal antibodies against the antigen-specific T cell receptor (TcR). Immobilized anti-receptor antibodies induced efficient enzyme secretion in the absence of target cells, thus implying a direct involvement of TcR complex in triggering exocytosis of granules. 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Psychology</subject><subject>Fundamental immunology</subject><subject>Immunobiology</subject><subject>Immunological reactions in vitro</subject><subject>Mice</subject><subject>Receptors, Antigen, T-Cell - physiology</subject><subject>Solubility</subject><subject>T-Lymphocytes, Cytotoxic - enzymology</subject><issn>0022-1767</issn><issn>1550-6606</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE9r3DAQxUVpSTd_vkChoEPpzVvJksb2MYSkCQRySW8FocijXQXbciUtG3_7qM02OeY0zLw3b4YfIV84W0smux-Pfhx3UxjWXLTreq0APpAVV4pVAAw-khVjdV3xBprP5DilR8YYsFoekSMhO94qviK_z6fsNzjRiBbnHGKVo99sMGJPE9qI2YeJBkcNzXGZk5-qvMxIMWWMJiF1MYzULjnk8OQtvafDMs7bUCaYTsknZ4aEZ4d6Qn5dXd5fXFe3dz9vLs5vKytkC5VpW8Yd75pWdF2jHmSjSqMsd30vwHHsJBjRG86giKJF4QyAAou8thJBnJDvL7lzDH925TU9-mRxGMyEYZd00wiQHN43cgkNF1IWY_1itDGkFNHpOfrRxEVzpv-y1__Z68Je11r9S_96SN89jNi_rhxgF_3bQTfJmsFFM1mfXm0tE10h8fbk1m-2ex9Rp9EMQwnler_fv917BrKCnKU</recordid><startdate>19870115</startdate><enddate>19870115</enddate><creator>Takayama, H</creator><creator>Trenn, G</creator><creator>Humphrey, W, Jr</creator><creator>Bluestone, JA</creator><creator>Henkart, PA</creator><creator>Sitkovsky, MV</creator><general>Am Assoc Immnol</general><general>American Association of Immunologists</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19870115</creationdate><title>Antigen receptor-triggered secretion of a trypsin-type esterase from cytotoxic T lymphocytes</title><author>Takayama, H ; Trenn, G ; Humphrey, W, Jr ; Bluestone, JA ; Henkart, PA ; Sitkovsky, MV</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3486-a8801f197839975b4751975c1fdd36f1e946a3da1065b438e3fa6656ce12c4e63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Cytoplasmic Granules - enzymology</topic><topic>Cytoplasmic Granules - physiology</topic><topic>Cytotoxic reactions (adcc reaction, cell-mediated lympholysis, complement-dependent cytotoxicity and others)</topic><topic>Cytotoxicity, Immunologic</topic><topic>Esterases - secretion</topic><topic>Exocytosis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Immunobiology</topic><topic>Immunological reactions in vitro</topic><topic>Mice</topic><topic>Receptors, Antigen, T-Cell - physiology</topic><topic>Solubility</topic><topic>T-Lymphocytes, Cytotoxic - enzymology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Takayama, H</creatorcontrib><creatorcontrib>Trenn, G</creatorcontrib><creatorcontrib>Humphrey, W, Jr</creatorcontrib><creatorcontrib>Bluestone, JA</creatorcontrib><creatorcontrib>Henkart, PA</creatorcontrib><creatorcontrib>Sitkovsky, MV</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of immunology (1950)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Takayama, H</au><au>Trenn, G</au><au>Humphrey, W, Jr</au><au>Bluestone, JA</au><au>Henkart, PA</au><au>Sitkovsky, MV</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antigen receptor-triggered secretion of a trypsin-type esterase from cytotoxic T lymphocytes</atitle><jtitle>The Journal of immunology (1950)</jtitle><addtitle>J Immunol</addtitle><date>1987-01-15</date><risdate>1987</risdate><volume>138</volume><issue>2</issue><spage>566</spage><epage>569</epage><pages>566-569</pages><issn>0022-1767</issn><eissn>1550-6606</eissn><coden>JOIMA3</coden><abstract>Exocytosis of cytolysin-containing granules from cytotoxic T lymphocytes (CTL) was studied with the use of granule enzyme (BLT esterase) as a convenient biochemical marker. Using cloned CTL, we demonstrate here that BLT esterase secretion into the supernatant is specifically triggered by antigen-bearing target cells and that this secretion is inhibited by soluble monoclonal antibodies against the antigen-specific T cell receptor (TcR). Immobilized anti-receptor antibodies induced efficient enzyme secretion in the absence of target cells, thus implying a direct involvement of TcR complex in triggering exocytosis of granules. These results support the role of the granule exocytosis in CTL functions and provide a quantitative and direct assay of a rapid CTL functional response to antigenic stimulation.</abstract><cop>Bethesda, MD</cop><pub>Am Assoc Immnol</pub><pmid>3491851</pmid><doi>10.4049/jimmunol.138.2.566</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of immunology (1950), 1987-01, Vol.138 (2), p.566-569 |
| issn | 0022-1767 1550-6606 |
| language | eng |
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| subjects | Animals Antibodies, Monoclonal Biological and medical sciences Cell Line Cytoplasmic Granules - enzymology Cytoplasmic Granules - physiology Cytotoxic reactions (adcc reaction, cell-mediated lympholysis, complement-dependent cytotoxicity and others) Cytotoxicity, Immunologic Esterases - secretion Exocytosis Fundamental and applied biological sciences. Psychology Fundamental immunology Immunobiology Immunological reactions in vitro Mice Receptors, Antigen, T-Cell - physiology Solubility T-Lymphocytes, Cytotoxic - enzymology |
| title | Antigen receptor-triggered secretion of a trypsin-type esterase from cytotoxic T lymphocytes |
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