A Continuous Spectrophotometric Assay for 5-Aminolevulinate Synthase That Utilizes Substrate Cycling

A continuous spectrophotometric assay for determining 5-aminolevulinic acid synthase activity is described. The assay is based upon coupling the production of coenzyme A by 5-aminolevulinic acid synthase to the reduction of NAD+ by α-ketoglutarate dehydrogenase and monitoring the increase in absorba...

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Veröffentlicht in:	Analytical biochemistry 1995-04, Vol.226 (2), p.221-224
Hauptverfasser:	Hunter, G.A., Ferreira, G.C.
Format:	Artikel
Sprache:	eng
Schlagworte:	5-Aminolevulinate Synthetase - metabolism Acyl Coenzyme A - metabolism Animals Coenzyme A - metabolism Glycine - metabolism Ketoglutarate Dehydrogenase Complex - metabolism Kinetics Mice NAD - metabolism Spectrophotometry, Ultraviolet - methods Substrate Cycling
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