Lovastatin Inhibits Proliferation and Differentiation and Causes Apoptosis in Lipopolysaccharide-Stimulated Murine B Cells

Lipopolysaccharide-stimulated murine B lymphocytes were incubated with the pharmacologic drug lovastatin, a competitive inhibitor of 3-hydroxy-3-methylglutaryl coenzyme A reductase at concentrations ranging from 10 −5-10 −8M. After 48 hr, treatment with 10 −5M lovastatin inhibited [ 3H] thymidine in...

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Veröffentlicht in:	Biochemical and biophysical research communications 1995-06, Vol.211 (2), p.665-670
Hauptverfasser:	Reedquist, K.A., Pope, T.K., Roess, D.A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Apoptosis - drug effects B-Lymphocytes - cytology B-Lymphocytes - drug effects B-Lymphocytes - physiology Cell Cycle - drug effects Cell Differentiation - drug effects Cell Division - drug effects Cells, Cultured DNA - biosynthesis DNA - drug effects Flow Cytometry Hemolytic Plaque Technique Hydroxymethylglutaryl-CoA Reductase Inhibitors Lipopolysaccharides - pharmacology Lovastatin - pharmacology Lymphocyte Activation - drug effects Lymphocyte Depletion Mice Spleen - immunology T-Lymphocytes Thymidine - metabolism
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creator	Reedquist, K.A. Pope, T.K. Roess, D.A.
description	Lipopolysaccharide-stimulated murine B lymphocytes were incubated with the pharmacologic drug lovastatin, a competitive inhibitor of 3-hydroxy-3-methylglutaryl coenzyme A reductase at concentrations ranging from 10 −5-10 −8M. After 48 hr, treatment with 10 −5M lovastatin inhibited [ 3H] thymidine incorporation 55%. Flow cytometric analysis indicated that the fraction of B cells in S phase was reduced from 26.7% ± 5.44% to 11.6% ± 2.9% when cells were incubated with 10 −5M lovastatin. In addition, a large peak appeared to the left of the G 1 peak that was indicative of apoptosis in cells treated with 10 −5M lovastatin. At 96 hr, the number of plaque-forming B cells was decreased 50% in the presence of lovastatin. Together these results suggest that high concentrations of lovastatin can inhibit B cell proliferation and differentiation of murine B lymphocytes in lipopolysaccharide-activated B cells, perhaps through induction of programmed cell death.
doi_str_mv	10.1006/bbrc.1995.1863
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After 48 hr, treatment with 10 −5M lovastatin inhibited [ 3H] thymidine incorporation 55%. Flow cytometric analysis indicated that the fraction of B cells in S phase was reduced from 26.7% ± 5.44% to 11.6% ± 2.9% when cells were incubated with 10 −5M lovastatin. In addition, a large peak appeared to the left of the G 1 peak that was indicative of apoptosis in cells treated with 10 −5M lovastatin. At 96 hr, the number of plaque-forming B cells was decreased 50% in the presence of lovastatin. Together these results suggest that high concentrations of lovastatin can inhibit B cell proliferation and differentiation of murine B lymphocytes in lipopolysaccharide-activated B cells, perhaps through induction of programmed cell death.</description><subject>Animals</subject><subject>Apoptosis - drug effects</subject><subject>B-Lymphocytes - cytology</subject><subject>B-Lymphocytes - drug effects</subject><subject>B-Lymphocytes - physiology</subject><subject>Cell Cycle - drug effects</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Division - drug effects</subject><subject>Cells, Cultured</subject><subject>DNA - biosynthesis</subject><subject>DNA - drug effects</subject><subject>Flow Cytometry</subject><subject>Hemolytic Plaque Technique</subject><subject>Hydroxymethylglutaryl-CoA Reductase Inhibitors</subject><subject>Lipopolysaccharides - pharmacology</subject><subject>Lovastatin - pharmacology</subject><subject>Lymphocyte Activation - drug effects</subject><subject>Lymphocyte Depletion</subject><subject>Mice</subject><subject>Spleen - immunology</subject><subject>T-Lymphocytes</subject><subject>Thymidine - metabolism</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM1r3DAQxUVpSTYf194KOvXmjWRbtnVMNm0S2NJCG8hNyNKYTPFaG40cSP_6atklOeU0vJk3j5kfY5-lWEohmou-j24ptVZL2TXVB7aQQouilKL-yBYiO4pSy4djdkL0Vwgp60YfsaO21XXZyQX7tw7PlpJNOPG76RF7TMR_xTDiADF3w8Tt5Pk1DlnDlPCtt7IzAfHLbdimQEg8R6wxqzC-kHXu0Ub0UPxOuJlHm8DzH3PECfgVX8E40hn7NNiR4PxQT9n9929_VrfF-ufN3epyXbiq0qloOyFa1VvrKtk3rpHe1nJoWqFlrwbvtIZaiUF6p8C70tum6aTyQpTW1sqV1Sn7us_dxvA0AyWzQXL5AjtBmMm0baUq1XbZuNwbXQxEEQazjbix8cVIYXawzQ622cE2O9h54cshee434F_tB7p53u3nkN97RoiGHMLkwGMEl4wP-F70fyGpkN0</recordid><startdate>19950615</startdate><enddate>19950615</enddate><creator>Reedquist, K.A.</creator><creator>Pope, T.K.</creator><creator>Roess, D.A.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19950615</creationdate><title>Lovastatin Inhibits Proliferation and Differentiation and Causes Apoptosis in Lipopolysaccharide-Stimulated Murine B Cells</title><author>Reedquist, K.A. ; Pope, T.K. ; Roess, D.A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c339t-780075baac31b6c61da41f67091b5fdc99e450f1dc5edc2da66815d002aa45c23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animals</topic><topic>Apoptosis - drug effects</topic><topic>B-Lymphocytes - cytology</topic><topic>B-Lymphocytes - drug effects</topic><topic>B-Lymphocytes - physiology</topic><topic>Cell Cycle - drug effects</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Division - drug effects</topic><topic>Cells, Cultured</topic><topic>DNA - biosynthesis</topic><topic>DNA - drug effects</topic><topic>Flow Cytometry</topic><topic>Hemolytic Plaque Technique</topic><topic>Hydroxymethylglutaryl-CoA Reductase Inhibitors</topic><topic>Lipopolysaccharides - pharmacology</topic><topic>Lovastatin - pharmacology</topic><topic>Lymphocyte Activation - drug effects</topic><topic>Lymphocyte Depletion</topic><topic>Mice</topic><topic>Spleen - immunology</topic><topic>T-Lymphocytes</topic><topic>Thymidine - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Reedquist, K.A.</creatorcontrib><creatorcontrib>Pope, T.K.</creatorcontrib><creatorcontrib>Roess, D.A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Reedquist, K.A.</au><au>Pope, T.K.</au><au>Roess, D.A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lovastatin Inhibits Proliferation and Differentiation and Causes Apoptosis in Lipopolysaccharide-Stimulated Murine B Cells</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1995-06-15</date><risdate>1995</risdate><volume>211</volume><issue>2</issue><spage>665</spage><epage>670</epage><pages>665-670</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Lipopolysaccharide-stimulated murine B lymphocytes were incubated with the pharmacologic drug lovastatin, a competitive inhibitor of 3-hydroxy-3-methylglutaryl coenzyme A reductase at concentrations ranging from 10 −5-10 −8M. After 48 hr, treatment with 10 −5M lovastatin inhibited [ 3H] thymidine incorporation 55%. Flow cytometric analysis indicated that the fraction of B cells in S phase was reduced from 26.7% ± 5.44% to 11.6% ± 2.9% when cells were incubated with 10 −5M lovastatin. In addition, a large peak appeared to the left of the G 1 peak that was indicative of apoptosis in cells treated with 10 −5M lovastatin. At 96 hr, the number of plaque-forming B cells was decreased 50% in the presence of lovastatin. Together these results suggest that high concentrations of lovastatin can inhibit B cell proliferation and differentiation of murine B lymphocytes in lipopolysaccharide-activated B cells, perhaps through induction of programmed cell death.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>7794281</pmid><doi>10.1006/bbrc.1995.1863</doi><tpages>6</tpages></addata></record>
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source	MEDLINE; ScienceDirect Journals (5 years ago - present)
subjects	Animals Apoptosis - drug effects B-Lymphocytes - cytology B-Lymphocytes - drug effects B-Lymphocytes - physiology Cell Cycle - drug effects Cell Differentiation - drug effects Cell Division - drug effects Cells, Cultured DNA - biosynthesis DNA - drug effects Flow Cytometry Hemolytic Plaque Technique Hydroxymethylglutaryl-CoA Reductase Inhibitors Lipopolysaccharides - pharmacology Lovastatin - pharmacology Lymphocyte Activation - drug effects Lymphocyte Depletion Mice Spleen - immunology T-Lymphocytes Thymidine - metabolism
title	Lovastatin Inhibits Proliferation and Differentiation and Causes Apoptosis in Lipopolysaccharide-Stimulated Murine B Cells
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