Recovery of Coccidioides immitis from blood and abscess fluid using the BacT/Alert system
We report the recovery of Coccidioides immitis from the blood and abscess fluid of two separate patients by using two automated blood culture systems. In the first case, an aspirate from a neck abscess containing C. immitis spherules was serially diluted and inoculated into liquid media used by the...
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| Veröffentlicht in: | Diagnostic microbiology and infectious disease 1995, Vol.21 (1), p.15-19 |
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| creator | Shawar, Ribhi M. Salyer, Jonny L. Ballou, Denise Clarridge, Jill E. |
| description | We report the recovery of
Coccidioides immitis from the blood and abscess fluid of two separate patients by using two automated blood culture systems. In the first case, an aspirate from a neck abscess containing
C. immitis spherules was serially diluted and inoculated into liquid media used by the BacT/Alert and the Bactec NR660 blood culture systems. BacT/Alert bottles inoculated with 10
5, 10
4, 10
3, 10
2, 10, and two spherules produced a positive signal at 19, 24, 35, 42, 57, and 62 h postinoculation, respectively. Bactec NR660 bottles containing >10
2 spherules and 10 spherules produced a positive signal after approximately 72 and 96 h of incubation, respectively. In the second case, a blood specimen incubated in BacT/Alert blood culture broth was signaled positive after 82 h of incubation. No organisms were detected by Gram stain of the broth, but
C. immitis grew after blind subculture. Our observations demonstrate that these rapid blood culture systems are capable of supporting growth of
C. immitis. To our knowledge, this report is the first to detect
C. immitis by these blood culture systems. |
| doi_str_mv | 10.1016/0732-8893(94)00112-A |
| format | Article |
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Coccidioides immitis from the blood and abscess fluid of two separate patients by using two automated blood culture systems. In the first case, an aspirate from a neck abscess containing
C. immitis spherules was serially diluted and inoculated into liquid media used by the BacT/Alert and the Bactec NR660 blood culture systems. BacT/Alert bottles inoculated with 10
5, 10
4, 10
3, 10
2, 10, and two spherules produced a positive signal at 19, 24, 35, 42, 57, and 62 h postinoculation, respectively. Bactec NR660 bottles containing >10
2 spherules and 10 spherules produced a positive signal after approximately 72 and 96 h of incubation, respectively. In the second case, a blood specimen incubated in BacT/Alert blood culture broth was signaled positive after 82 h of incubation. No organisms were detected by Gram stain of the broth, but
C. immitis grew after blind subculture. Our observations demonstrate that these rapid blood culture systems are capable of supporting growth of
C. immitis. To our knowledge, this report is the first to detect
C. immitis by these blood culture systems.</description><identifier>ISSN: 0732-8893</identifier><identifier>EISSN: 1879-0070</identifier><identifier>DOI: 10.1016/0732-8893(94)00112-A</identifier><identifier>PMID: 7789092</identifier><identifier>CODEN: DMIDDZ</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Adult ; Biological and medical sciences ; Coccidioides - isolation & purification ; Coccidioidomycosis - diagnosis ; Culture Media ; Fungemia - diagnosis ; Fungemia - microbiology ; Human mycoses ; Humans ; Infectious diseases ; Lung Abscess - diagnosis ; Lung Abscess - microbiology ; Male ; Medical sciences ; Microbiological Techniques ; Middle Aged ; Mycoses ; Mycotic sepsis</subject><ispartof>Diagnostic microbiology and infectious disease, 1995, Vol.21 (1), p.15-19</ispartof><rights>1995</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-71170338c3b02effac7157bbdccddb49dd4190e6e2388f0331b011f1c657ab6e3</citedby><cites>FETCH-LOGICAL-c386t-71170338c3b02effac7157bbdccddb49dd4190e6e2388f0331b011f1c657ab6e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/073288939400112A$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,4010,27902,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3473844$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7789092$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shawar, Ribhi M.</creatorcontrib><creatorcontrib>Salyer, Jonny L.</creatorcontrib><creatorcontrib>Ballou, Denise</creatorcontrib><creatorcontrib>Clarridge, Jill E.</creatorcontrib><title>Recovery of Coccidioides immitis from blood and abscess fluid using the BacT/Alert system</title><title>Diagnostic microbiology and infectious disease</title><addtitle>Diagn Microbiol Infect Dis</addtitle><description>We report the recovery of
Coccidioides immitis from the blood and abscess fluid of two separate patients by using two automated blood culture systems. In the first case, an aspirate from a neck abscess containing
C. immitis spherules was serially diluted and inoculated into liquid media used by the BacT/Alert and the Bactec NR660 blood culture systems. BacT/Alert bottles inoculated with 10
5, 10
4, 10
3, 10
2, 10, and two spherules produced a positive signal at 19, 24, 35, 42, 57, and 62 h postinoculation, respectively. Bactec NR660 bottles containing >10
2 spherules and 10 spherules produced a positive signal after approximately 72 and 96 h of incubation, respectively. In the second case, a blood specimen incubated in BacT/Alert blood culture broth was signaled positive after 82 h of incubation. No organisms were detected by Gram stain of the broth, but
C. immitis grew after blind subculture. Our observations demonstrate that these rapid blood culture systems are capable of supporting growth of
C. immitis. To our knowledge, this report is the first to detect
C. immitis by these blood culture systems.</description><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Coccidioides - isolation & purification</subject><subject>Coccidioidomycosis - diagnosis</subject><subject>Culture Media</subject><subject>Fungemia - diagnosis</subject><subject>Fungemia - microbiology</subject><subject>Human mycoses</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Lung Abscess - diagnosis</subject><subject>Lung Abscess - microbiology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Microbiological Techniques</subject><subject>Middle Aged</subject><subject>Mycoses</subject><subject>Mycotic sepsis</subject><issn>0732-8893</issn><issn>1879-0070</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE2LFDEQQIMo67j6DxRyENFDu0kn20kuwjj4BQuCrAdPIalUNNLdWZPuhfn3Zpxhjh6KgqpXRdUj5DlnbznjwxVTou-0NuK1kW8Y47zvtg_IhmtlOsYUe0g2Z-QxeVLr7wb1RrILcqGUNsz0G_LjG0K-x7KnOdJdBkgh5RSw0jRNaUmVxpIn6secA3VzC18BayuPawp0rWn-SZdfSN87uL3ajlgWWvd1wekpeRTdWPHZKV-S7x8_3O4-dzdfP33ZbW86EHpYOsW5YkJoEJ71GKMDxa-V9wEgBC9NCJIbhgP2QuvYSO7bq5HDcK2cH1BcklfHvXcl_1mxLnZK7cRxdDPmtVqlhGzP8gbKIwgl11ow2ruSJlf2ljN7MGoPuuxBlzXS_jNqt23sxWn_6icM56GTwtZ_eeq7Cm6Mxc2Q6hkTUgktZcPeHTFsLu4TFlsh4QwYUkFYbMjp_3f8BQ2Mkgw</recordid><startdate>1995</startdate><enddate>1995</enddate><creator>Shawar, Ribhi M.</creator><creator>Salyer, Jonny L.</creator><creator>Ballou, Denise</creator><creator>Clarridge, Jill E.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1995</creationdate><title>Recovery of Coccidioides immitis from blood and abscess fluid using the BacT/Alert system</title><author>Shawar, Ribhi M. ; Salyer, Jonny L. ; Ballou, Denise ; Clarridge, Jill E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-71170338c3b02effac7157bbdccddb49dd4190e6e2388f0331b011f1c657ab6e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>Coccidioides - isolation & purification</topic><topic>Coccidioidomycosis - diagnosis</topic><topic>Culture Media</topic><topic>Fungemia - diagnosis</topic><topic>Fungemia - microbiology</topic><topic>Human mycoses</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Lung Abscess - diagnosis</topic><topic>Lung Abscess - microbiology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Microbiological Techniques</topic><topic>Middle Aged</topic><topic>Mycoses</topic><topic>Mycotic sepsis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shawar, Ribhi M.</creatorcontrib><creatorcontrib>Salyer, Jonny L.</creatorcontrib><creatorcontrib>Ballou, Denise</creatorcontrib><creatorcontrib>Clarridge, Jill E.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Diagnostic microbiology and infectious disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shawar, Ribhi M.</au><au>Salyer, Jonny L.</au><au>Ballou, Denise</au><au>Clarridge, Jill E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Recovery of Coccidioides immitis from blood and abscess fluid using the BacT/Alert system</atitle><jtitle>Diagnostic microbiology and infectious disease</jtitle><addtitle>Diagn Microbiol Infect Dis</addtitle><date>1995</date><risdate>1995</risdate><volume>21</volume><issue>1</issue><spage>15</spage><epage>19</epage><pages>15-19</pages><issn>0732-8893</issn><eissn>1879-0070</eissn><coden>DMIDDZ</coden><abstract>We report the recovery of
Coccidioides immitis from the blood and abscess fluid of two separate patients by using two automated blood culture systems. In the first case, an aspirate from a neck abscess containing
C. immitis spherules was serially diluted and inoculated into liquid media used by the BacT/Alert and the Bactec NR660 blood culture systems. BacT/Alert bottles inoculated with 10
5, 10
4, 10
3, 10
2, 10, and two spherules produced a positive signal at 19, 24, 35, 42, 57, and 62 h postinoculation, respectively. Bactec NR660 bottles containing >10
2 spherules and 10 spherules produced a positive signal after approximately 72 and 96 h of incubation, respectively. In the second case, a blood specimen incubated in BacT/Alert blood culture broth was signaled positive after 82 h of incubation. No organisms were detected by Gram stain of the broth, but
C. immitis grew after blind subculture. Our observations demonstrate that these rapid blood culture systems are capable of supporting growth of
C. immitis. To our knowledge, this report is the first to detect
C. immitis by these blood culture systems.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>7789092</pmid><doi>10.1016/0732-8893(94)00112-A</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0732-8893 |
| ispartof | Diagnostic microbiology and infectious disease, 1995, Vol.21 (1), p.15-19 |
| issn | 0732-8893 1879-0070 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77348901 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adult Biological and medical sciences Coccidioides - isolation & purification Coccidioidomycosis - diagnosis Culture Media Fungemia - diagnosis Fungemia - microbiology Human mycoses Humans Infectious diseases Lung Abscess - diagnosis Lung Abscess - microbiology Male Medical sciences Microbiological Techniques Middle Aged Mycoses Mycotic sepsis |
| title | Recovery of Coccidioides immitis from blood and abscess fluid using the BacT/Alert system |
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