A mini-promoter lacZ gene fusion for the analysis of fungal transcription control sequences

A system for the in vivo analysis of fungal transcription control sequences, based on a mini-promoter, was designed. The mini-promoter, providing all sequences necessary and sufficient for transcription initiation, was derived from the Aspergillus nidulans gpdA promoter region. Transcription initiat...

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Veröffentlicht in:	Gene 1995, Vol.158 (1), p.119-123
Hauptverfasser:	Punt, Peter J., Kuyvenhoven, Anneke, van den Hondel, Cees A.M.J.J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Aspergillus nidulans Aspergillus nidulans - genetics Aspergillus nidulellus Base Sequence beta-galactosidase carbon regulation Cloning, Molecular - methods deletions Gene Expression Regulation, Fungal genetic transformation Genetic Vectors Glyceraldehyde-3-Phosphate Dehydrogenases - genetics gpd box gpda promoters Lac Operon lacZ reporter gene Molecular Sequence Data nitrogen regulation Oligodeoxyribonucleotides plasmid vectors promoter regions Promoter Regions, Genetic Recombinant DNA reporter genes targeted single-copy integration transcription (genetics) transcription initiation transcription start point(s) Transcription, Genetic - genetics upstream activating sequences β-galactosidase
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