Galanin is contained in GABAergic neurons in the rat spinal dorsal horn
In order to determine which types of neuron in laminae I–III of the rat spinal dorsal horn contain the peptide galanin, pre-embedding immunocytochemistry with antiserum to galanin was combined with post-embedding detection of GABA- and glycine-like immunoreactivities. Sixty-eight galanin immunoreact...
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| Veröffentlicht in: | Neuroscience letters 1995-03, Vol.187 (2), p.119-122 |
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| creator | Simmons, D.R. Spike, R.C. Todd, A.J. |
| description | In order to determine which types of neuron in laminae I–III of the rat spinal dorsal horn contain the peptide galanin, pre-embedding immunocytochemistry with antiserum to galanin was combined with post-embedding detection of GABA- and glycine-like immunoreactivities. Sixty-eight galanin immunoreactive neurons in laminae I–III selected from four rats were examined, and in each case semi-thin sections through the cell body were tested with a monoclonal antibody to GABA and an antiserum to glycine. All of the 68 galanin-immunoreactive neurons tested were GABA-immunoreactive, while only one of them (in lamina III) was glycine-immunoreactive. This suggests that galanin is contained in inhibitory interneurons, and that (like enkephalin, neuropeptide Y and thyrotropin-releasing hormone) it is mainly restricted to GABAergic neurons which do not use glycine as a co-transmitter. |
| doi_str_mv | 10.1016/0304-3940(95)11358-4 |
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Sixty-eight galanin immunoreactive neurons in laminae I–III selected from four rats were examined, and in each case semi-thin sections through the cell body were tested with a monoclonal antibody to GABA and an antiserum to glycine. All of the 68 galanin-immunoreactive neurons tested were GABA-immunoreactive, while only one of them (in lamina III) was glycine-immunoreactive. This suggests that galanin is contained in inhibitory interneurons, and that (like enkephalin, neuropeptide Y and thyrotropin-releasing hormone) it is mainly restricted to GABAergic neurons which do not use glycine as a co-transmitter.</description><identifier>ISSN: 0304-3940</identifier><identifier>EISSN: 1872-7972</identifier><identifier>DOI: 10.1016/0304-3940(95)11358-4</identifier><identifier>PMID: 7540270</identifier><identifier>CODEN: NELED5</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Animals ; Biological and medical sciences ; Central nervous system ; Central neurotransmission. Neuromudulation. Pathways and receptors ; Female ; Fundamental and applied biological sciences. Psychology ; Galanin ; gamma-Aminobutyric Acid - metabolism ; Glycine ; Immunocytochemistry ; Immunohistochemistry ; Interneurons - metabolism ; Male ; Neurons - metabolism ; Neuropeptides - metabolism ; Peptides ; Peptides - metabolism ; Rats ; Rats, Inbred Strains ; Spinal cord ; Spinal Cord - cytology ; Spinal Cord - metabolism ; Tissue Distribution ; Transmitter co-localisation ; Vertebrates: nervous system and sense organs</subject><ispartof>Neuroscience letters, 1995-03, Vol.187 (2), p.119-122</ispartof><rights>1995</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c483t-4b1ec11d1da934f37bd331d298e345845e2bf8e39ec9027585abfd7e4c9272d3</citedby><cites>FETCH-LOGICAL-c483t-4b1ec11d1da934f37bd331d298e345845e2bf8e39ec9027585abfd7e4c9272d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0304394095113584$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3448462$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7540270$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Simmons, D.R.</creatorcontrib><creatorcontrib>Spike, R.C.</creatorcontrib><creatorcontrib>Todd, A.J.</creatorcontrib><title>Galanin is contained in GABAergic neurons in the rat spinal dorsal horn</title><title>Neuroscience letters</title><addtitle>Neurosci Lett</addtitle><description>In order to determine which types of neuron in laminae I–III of the rat spinal dorsal horn contain the peptide galanin, pre-embedding immunocytochemistry with antiserum to galanin was combined with post-embedding detection of GABA- and glycine-like immunoreactivities. Sixty-eight galanin immunoreactive neurons in laminae I–III selected from four rats were examined, and in each case semi-thin sections through the cell body were tested with a monoclonal antibody to GABA and an antiserum to glycine. All of the 68 galanin-immunoreactive neurons tested were GABA-immunoreactive, while only one of them (in lamina III) was glycine-immunoreactive. This suggests that galanin is contained in inhibitory interneurons, and that (like enkephalin, neuropeptide Y and thyrotropin-releasing hormone) it is mainly restricted to GABAergic neurons which do not use glycine as a co-transmitter.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Central nervous system</subject><subject>Central neurotransmission. Neuromudulation. Pathways and receptors</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Galanin</subject><subject>gamma-Aminobutyric Acid - metabolism</subject><subject>Glycine</subject><subject>Immunocytochemistry</subject><subject>Immunohistochemistry</subject><subject>Interneurons - metabolism</subject><subject>Male</subject><subject>Neurons - metabolism</subject><subject>Neuropeptides - metabolism</subject><subject>Peptides</subject><subject>Peptides - metabolism</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Spinal cord</subject><subject>Spinal Cord - cytology</subject><subject>Spinal Cord - metabolism</subject><subject>Tissue Distribution</subject><subject>Transmitter co-localisation</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0304-3940</issn><issn>1872-7972</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1vGyEQhlHUyHU-_kEq7aGqksMmsAwGLpFcq3UiRcold8TCbEK1Zl1YR8q_L1tbPrangZmHV8NDyBWjt4yyxR3lFGqugV5rccMYF6qGEzJnSja11LL5ROZH5DM5y_kXpVQwATMykwJoI-mcrNe2tzHEKuTKDXG0IaKvyn29_L7E9BpcFXGXhpin5viGVbJjlbch2r7yQ8qlvA0pXpDTzvYZLw_1nLz8_PGyeqifntePq-VT7UDxsYaWoWPMM281h47L1nPOfKMVchAKBDZtV84anS4LCiVs23mJ4HQjG8_Pybd97DYNv3eYR7MJ2WFf_oDDLhspOXBG4b8gWyjNlBYFhD3o0pBzws5sU9jY9GEYNZNnM0k0k0Sjhfnr2Uz5Xw75u3aD_vjoILbMvx7mNjvbd8lGF_IR4wAKFk3B7vcYFmfvAZPJLmB06ENCNxo_hH_v8QdLwpdq</recordid><startdate>19950303</startdate><enddate>19950303</enddate><creator>Simmons, D.R.</creator><creator>Spike, R.C.</creator><creator>Todd, A.J.</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19950303</creationdate><title>Galanin is contained in GABAergic neurons in the rat spinal dorsal horn</title><author>Simmons, D.R. ; Spike, R.C. ; Todd, A.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c483t-4b1ec11d1da934f37bd331d298e345845e2bf8e39ec9027585abfd7e4c9272d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Central nervous system</topic><topic>Central neurotransmission. Neuromudulation. Pathways and receptors</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Galanin</topic><topic>gamma-Aminobutyric Acid - metabolism</topic><topic>Glycine</topic><topic>Immunocytochemistry</topic><topic>Immunohistochemistry</topic><topic>Interneurons - metabolism</topic><topic>Male</topic><topic>Neurons - metabolism</topic><topic>Neuropeptides - metabolism</topic><topic>Peptides</topic><topic>Peptides - metabolism</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Spinal cord</topic><topic>Spinal Cord - cytology</topic><topic>Spinal Cord - metabolism</topic><topic>Tissue Distribution</topic><topic>Transmitter co-localisation</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Simmons, D.R.</creatorcontrib><creatorcontrib>Spike, R.C.</creatorcontrib><creatorcontrib>Todd, A.J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Simmons, D.R.</au><au>Spike, R.C.</au><au>Todd, A.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Galanin is contained in GABAergic neurons in the rat spinal dorsal horn</atitle><jtitle>Neuroscience letters</jtitle><addtitle>Neurosci Lett</addtitle><date>1995-03-03</date><risdate>1995</risdate><volume>187</volume><issue>2</issue><spage>119</spage><epage>122</epage><pages>119-122</pages><issn>0304-3940</issn><eissn>1872-7972</eissn><coden>NELED5</coden><abstract>In order to determine which types of neuron in laminae I–III of the rat spinal dorsal horn contain the peptide galanin, pre-embedding immunocytochemistry with antiserum to galanin was combined with post-embedding detection of GABA- and glycine-like immunoreactivities. Sixty-eight galanin immunoreactive neurons in laminae I–III selected from four rats were examined, and in each case semi-thin sections through the cell body were tested with a monoclonal antibody to GABA and an antiserum to glycine. All of the 68 galanin-immunoreactive neurons tested were GABA-immunoreactive, while only one of them (in lamina III) was glycine-immunoreactive. This suggests that galanin is contained in inhibitory interneurons, and that (like enkephalin, neuropeptide Y and thyrotropin-releasing hormone) it is mainly restricted to GABAergic neurons which do not use glycine as a co-transmitter.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>7540270</pmid><doi>10.1016/0304-3940(95)11358-4</doi><tpages>4</tpages></addata></record> |
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| ispartof | Neuroscience letters, 1995-03, Vol.187 (2), p.119-122 |
| issn | 0304-3940 1872-7972 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Biological and medical sciences Central nervous system Central neurotransmission. Neuromudulation. Pathways and receptors Female Fundamental and applied biological sciences. Psychology Galanin gamma-Aminobutyric Acid - metabolism Glycine Immunocytochemistry Immunohistochemistry Interneurons - metabolism Male Neurons - metabolism Neuropeptides - metabolism Peptides Peptides - metabolism Rats Rats, Inbred Strains Spinal cord Spinal Cord - cytology Spinal Cord - metabolism Tissue Distribution Transmitter co-localisation Vertebrates: nervous system and sense organs |
| title | Galanin is contained in GABAergic neurons in the rat spinal dorsal horn |
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