Expression of Cytochrome P450 2D6 in Escherichia coli, Purification, and Spectral and Catalytic Characterization

Cytochrome P450 (P450) 2D6 is the classic human liver debrisoquine 4-hydroxylase, the first human P450 for which genetic polymorphism was clearly demonstrated. We prepared 11 different constructs of P450 2D6, with modification at the N-terminus, for expression in Escherichia coli with the vector pCW...

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Veröffentlicht in:	Archives of biochemistry and biophysics 1995-06, Vol.319 (2), p.540-550
Hauptverfasser:	Gillam, E.M.J., Guo, Z.Y., Martin, M.V., Jenkins, C.M., Guengerich, F.P.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Base Sequence Cytochrome P-450 CYP2D6 Cytochrome P-450 Enzyme System - chemistry Cytochrome P-450 Enzyme System - genetics Cytochrome P-450 Enzyme System - isolation & purification DNA Primers Escherichia coli - enzymology Escherichia coli - genetics Gene Expression Gene Transfer Techniques Humans Mixed Function Oxygenases - chemistry Mixed Function Oxygenases - genetics Mixed Function Oxygenases - isolation & purification Molecular Sequence Data Plasmids Polymerase Chain Reaction
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