Genetic stability of cold-adapted A/Leningrad/134/47/57 (H2N2) influenza virus: sequence analysis of live cold-adapted reassortant vaccine strains before and after replication in children
1 Influenza Branch G-16, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA 2 Research Institute for Viral Preparations, Russian Academy of Medicial Sciences, Moscow 109088, Russia and 3 Researc...
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| Veröffentlicht in: | Journal of general virology 1995-06, Vol.76 (6), p.1521-1525 |
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| container_title | Journal of general virology |
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| creator | Klimov, Alexander I Egorov, Andrei Y Gushchina, Marina I Medvedeva, Tamila E Gamble, William C Rudenko, Larisa G Alexandrova, Galina I Cox, Nancy J |
| description | 1 Influenza Branch G-16, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
2 Research Institute for Viral Preparations, Russian Academy of Medicial Sciences, Moscow 109088, Russia
and 3 Research Institute for Experimental Medicine, Russian Academy of Medical Sciences, St Petersburg 197022, Russia
We previously reported that the A/Leningrad/134/47/57 (H2N2) cold-adapted virus (A/Len/47) used in preparing reassortant live attenuated vaccines for children acquired 14 (11 coding) mutations in genes coding for proteins other than haemagglutinin and neuraminidase during cold-adaptation. Preservation of these mutations in genomes of viruses isolated from children on the second, fifth, or eighth day after vaccination was examined by sequence analysis. The sequence data demonstrated that all nine coding mutations selected for examination were conserved in the genomes of all 11 strains investigated, indicating that the mutations accompanying cold-adaptation and attenuation of the A/Len/47 master vaccine are highly stable.
* Author for correspondence. Fax +1 404 639 2334. e-mail njcl@ciddvdl
Received 24 October 1994;
accepted 21 February 1995. |
| doi_str_mv | 10.1099/0022-1317-76-6-1521 |
| format | Article |
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2 Research Institute for Viral Preparations, Russian Academy of Medicial Sciences, Moscow 109088, Russia
and 3 Research Institute for Experimental Medicine, Russian Academy of Medical Sciences, St Petersburg 197022, Russia
We previously reported that the A/Leningrad/134/47/57 (H2N2) cold-adapted virus (A/Len/47) used in preparing reassortant live attenuated vaccines for children acquired 14 (11 coding) mutations in genes coding for proteins other than haemagglutinin and neuraminidase during cold-adaptation. Preservation of these mutations in genomes of viruses isolated from children on the second, fifth, or eighth day after vaccination was examined by sequence analysis. The sequence data demonstrated that all nine coding mutations selected for examination were conserved in the genomes of all 11 strains investigated, indicating that the mutations accompanying cold-adaptation and attenuation of the A/Len/47 master vaccine are highly stable.
* Author for correspondence. Fax +1 404 639 2334. e-mail njcl@ciddvdl
Received 24 October 1994;
accepted 21 February 1995.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/0022-1317-76-6-1521</identifier><identifier>PMID: 7782782</identifier><language>eng</language><publisher>England: Soc General Microbiol</publisher><subject>Acclimatization ; Amino Acid Sequence ; Base Sequence ; Child ; Codon ; Cold Temperature ; Conserved Sequence ; DNA Primers ; Genome, Viral ; Humans ; influenza A virus ; Influenza A virus - genetics ; Influenza A virus - immunology ; Influenza A virus - physiology ; Influenza A Virus, H2N2 Subtype ; Influenza Vaccines ; Molecular Sequence Data ; Polymerase Chain Reaction ; Russia ; Vaccines, Attenuated ; Virus Replication</subject><ispartof>Journal of general virology, 1995-06, Vol.76 (6), p.1521-1525</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c409t-45d67d423ae1a7049a4fd5801ae49aa2d7ec62445f4f55630fa383e94632c15a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3733,3734,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7782782$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Klimov, Alexander I</creatorcontrib><creatorcontrib>Egorov, Andrei Y</creatorcontrib><creatorcontrib>Gushchina, Marina I</creatorcontrib><creatorcontrib>Medvedeva, Tamila E</creatorcontrib><creatorcontrib>Gamble, William C</creatorcontrib><creatorcontrib>Rudenko, Larisa G</creatorcontrib><creatorcontrib>Alexandrova, Galina I</creatorcontrib><creatorcontrib>Cox, Nancy J</creatorcontrib><title>Genetic stability of cold-adapted A/Leningrad/134/47/57 (H2N2) influenza virus: sequence analysis of live cold-adapted reassortant vaccine strains before and after replication in children</title><title>Journal of general virology</title><addtitle>J Gen Virol</addtitle><description>1 Influenza Branch G-16, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
2 Research Institute for Viral Preparations, Russian Academy of Medicial Sciences, Moscow 109088, Russia
and 3 Research Institute for Experimental Medicine, Russian Academy of Medical Sciences, St Petersburg 197022, Russia
We previously reported that the A/Leningrad/134/47/57 (H2N2) cold-adapted virus (A/Len/47) used in preparing reassortant live attenuated vaccines for children acquired 14 (11 coding) mutations in genes coding for proteins other than haemagglutinin and neuraminidase during cold-adaptation. Preservation of these mutations in genomes of viruses isolated from children on the second, fifth, or eighth day after vaccination was examined by sequence analysis. The sequence data demonstrated that all nine coding mutations selected for examination were conserved in the genomes of all 11 strains investigated, indicating that the mutations accompanying cold-adaptation and attenuation of the A/Len/47 master vaccine are highly stable.
* Author for correspondence. Fax +1 404 639 2334. e-mail njcl@ciddvdl
Received 24 October 1994;
accepted 21 February 1995.</description><subject>Acclimatization</subject><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>Child</subject><subject>Codon</subject><subject>Cold Temperature</subject><subject>Conserved Sequence</subject><subject>DNA Primers</subject><subject>Genome, Viral</subject><subject>Humans</subject><subject>influenza A virus</subject><subject>Influenza A virus - genetics</subject><subject>Influenza A virus - immunology</subject><subject>Influenza A virus - physiology</subject><subject>Influenza A Virus, H2N2 Subtype</subject><subject>Influenza Vaccines</subject><subject>Molecular Sequence Data</subject><subject>Polymerase Chain Reaction</subject><subject>Russia</subject><subject>Vaccines, Attenuated</subject><subject>Virus Replication</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkd-KEzEUxgdR1u7qE4iQK9m9GCf_Jpnxbll0Vyh6o9fhNDnTRtJMTaZd6qv5cmZoWfFKCCSH7zvfSfKrqjeMvme07xtKOa-ZYLrWqlY1azl7Vi2YVG3Ni_68Wjw5XlaXOf-glEnZ6ovqQuuOl7Woft9jxMlbkidY-eCnIxkHYsfganCwm9CR22aJ0cd1AtcwIRupm1aT6wf-hd8QH4ewx_gLyMGnff5AMv4stUUCEcIx-zznBX_Af0MTQs5jmiBO5ADW-ojlCgl8zGSFw5jmAEdgmDAV8y54C5MfYxlI7MYHlzC-ql4MEDK-Pu9X1fdPH7_dPdTLr_ef726XtZW0n2rZOqWd5AKQgaayBzm4tqMMsJyBO41W8fIxgxzaVgk6gOgE9lIJblkL4qp6d8rdpbE8Lk9m67PFECDiuM9GayGkVPq_RqY6qbq2K0ZxMto05pxwMLvkt5COhlEzszUzOTOTM1oZZWa2pevtOX6_2qJ76jnDLPr1Sd_49ebRJzRrjFtfZqz8aAqfv1F_AP3SrpQ</recordid><startdate>19950601</startdate><enddate>19950601</enddate><creator>Klimov, Alexander I</creator><creator>Egorov, Andrei Y</creator><creator>Gushchina, Marina I</creator><creator>Medvedeva, Tamila E</creator><creator>Gamble, William C</creator><creator>Rudenko, Larisa G</creator><creator>Alexandrova, Galina I</creator><creator>Cox, Nancy J</creator><general>Soc General Microbiol</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19950601</creationdate><title>Genetic stability of cold-adapted A/Leningrad/134/47/57 (H2N2) influenza virus: sequence analysis of live cold-adapted reassortant vaccine strains before and after replication in children</title><author>Klimov, Alexander I ; Egorov, Andrei Y ; Gushchina, Marina I ; Medvedeva, Tamila E ; Gamble, William C ; Rudenko, Larisa G ; Alexandrova, Galina I ; Cox, Nancy J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-45d67d423ae1a7049a4fd5801ae49aa2d7ec62445f4f55630fa383e94632c15a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Acclimatization</topic><topic>Amino Acid Sequence</topic><topic>Base Sequence</topic><topic>Child</topic><topic>Codon</topic><topic>Cold Temperature</topic><topic>Conserved Sequence</topic><topic>DNA Primers</topic><topic>Genome, Viral</topic><topic>Humans</topic><topic>influenza A virus</topic><topic>Influenza A virus - genetics</topic><topic>Influenza A virus - immunology</topic><topic>Influenza A virus - physiology</topic><topic>Influenza A Virus, H2N2 Subtype</topic><topic>Influenza Vaccines</topic><topic>Molecular Sequence Data</topic><topic>Polymerase Chain Reaction</topic><topic>Russia</topic><topic>Vaccines, Attenuated</topic><topic>Virus Replication</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Klimov, Alexander I</creatorcontrib><creatorcontrib>Egorov, Andrei Y</creatorcontrib><creatorcontrib>Gushchina, Marina I</creatorcontrib><creatorcontrib>Medvedeva, Tamila E</creatorcontrib><creatorcontrib>Gamble, William C</creatorcontrib><creatorcontrib>Rudenko, Larisa G</creatorcontrib><creatorcontrib>Alexandrova, Galina I</creatorcontrib><creatorcontrib>Cox, Nancy J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Klimov, Alexander I</au><au>Egorov, Andrei Y</au><au>Gushchina, Marina I</au><au>Medvedeva, Tamila E</au><au>Gamble, William C</au><au>Rudenko, Larisa G</au><au>Alexandrova, Galina I</au><au>Cox, Nancy J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic stability of cold-adapted A/Leningrad/134/47/57 (H2N2) influenza virus: sequence analysis of live cold-adapted reassortant vaccine strains before and after replication in children</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>1995-06-01</date><risdate>1995</risdate><volume>76</volume><issue>6</issue><spage>1521</spage><epage>1525</epage><pages>1521-1525</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><abstract>1 Influenza Branch G-16, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
2 Research Institute for Viral Preparations, Russian Academy of Medicial Sciences, Moscow 109088, Russia
and 3 Research Institute for Experimental Medicine, Russian Academy of Medical Sciences, St Petersburg 197022, Russia
We previously reported that the A/Leningrad/134/47/57 (H2N2) cold-adapted virus (A/Len/47) used in preparing reassortant live attenuated vaccines for children acquired 14 (11 coding) mutations in genes coding for proteins other than haemagglutinin and neuraminidase during cold-adaptation. Preservation of these mutations in genomes of viruses isolated from children on the second, fifth, or eighth day after vaccination was examined by sequence analysis. The sequence data demonstrated that all nine coding mutations selected for examination were conserved in the genomes of all 11 strains investigated, indicating that the mutations accompanying cold-adaptation and attenuation of the A/Len/47 master vaccine are highly stable.
* Author for correspondence. Fax +1 404 639 2334. e-mail njcl@ciddvdl
Received 24 October 1994;
accepted 21 February 1995.</abstract><cop>England</cop><pub>Soc General Microbiol</pub><pmid>7782782</pmid><doi>10.1099/0022-1317-76-6-1521</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Microbiology Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Acclimatization Amino Acid Sequence Base Sequence Child Codon Cold Temperature Conserved Sequence DNA Primers Genome, Viral Humans influenza A virus Influenza A virus - genetics Influenza A virus - immunology Influenza A virus - physiology Influenza A Virus, H2N2 Subtype Influenza Vaccines Molecular Sequence Data Polymerase Chain Reaction Russia Vaccines, Attenuated Virus Replication |
| title | Genetic stability of cold-adapted A/Leningrad/134/47/57 (H2N2) influenza virus: sequence analysis of live cold-adapted reassortant vaccine strains before and after replication in children |
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