Immunohistochemical Localization of Serotonin in the Third Ventricular Wall of the Lamprey, Using Backscattered Electron Imaging
The distribution and fine surface structures of serotonin-immunoreactive cells in the third ventricular wall of the lamprey, Lampetra japonica, were examined by backscattered electron (BSE) imaging of immunohistochemical reactions as well as by a secondary emission image of conventional scanning ele...
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| Veröffentlicht in: | Journal of electron microscopy 1986, Vol.35 (2), p.157-164 |
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| container_title | Journal of electron microscopy |
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| creator | USHIKI, Tatsuo YUI, Ryogo FUJITA, Tsuneo |
| description | The distribution and fine surface structures of serotonin-immunoreactive cells in the third ventricular wall of the lamprey, Lampetra japonica, were examined by backscattered electron (BSE) imaging of immunohistochemical reactions as well as by a secondary emission image of conventional scanning electron microscopy (SEM). The BSE image of immunohistochemical specimens clearly visualized the immunostained structures as bright spots which corresponded to the bulbous terminals of the cerebrospinal fluid (CSF)-contacting neurons. These terminals were grouped in the hypothalamic area on the border of the ventral thalamus caudal to the level of the postoptic commissure. Secondary emission images of conventionally prepared specimens revealed a well-developed labyrinthine structure of nerve terminals and fibers in the corresponding area. The location, surface structure and serotonin content in the nerves showed a similarity between this region of the lamprey and the paraventricular organ of other higher vertebrate classes. |
| doi_str_mv | 10.1093/oxfordjournals.jmicro.a050561 |
| format | Article |
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The BSE image of immunohistochemical specimens clearly visualized the immunostained structures as bright spots which corresponded to the bulbous terminals of the cerebrospinal fluid (CSF)-contacting neurons. These terminals were grouped in the hypothalamic area on the border of the ventral thalamus caudal to the level of the postoptic commissure. Secondary emission images of conventionally prepared specimens revealed a well-developed labyrinthine structure of nerve terminals and fibers in the corresponding area. The location, surface structure and serotonin content in the nerves showed a similarity between this region of the lamprey and the paraventricular organ of other higher vertebrate classes.</description><identifier>ISSN: 0022-0744</identifier><identifier>ISSN: 1477-9986</identifier><identifier>EISSN: 1477-9986</identifier><identifier>DOI: 10.1093/oxfordjournals.jmicro.a050561</identifier><identifier>PMID: 3553411</identifier><identifier>CODEN: JELJA7</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Animals ; backscattered electron image ; Biological and medical sciences ; Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges ; Cerebral Ventricles - analysis ; Cerebral Ventricles - ultrastructure ; cerebrospinal fluid (CSF)-contacting neurons ; Female ; Fundamental and applied biological sciences. Psychology ; Image Enhancement - methods ; Immunoenzyme Techniques ; Lampetra japonica ; Lampreys ; Male ; Microscopy, Electron, Scanning - methods ; Neurons - analysis ; Neurons - ultrastructure ; paraventricular organ ; Serotonin - analysis ; serotonin-immunoreactivity ; Vertebrates: nervous system and sense organs</subject><ispartof>Journal of electron microscopy, 1986, Vol.35 (2), p.157-164</ispartof><rights>1987 INIST-CNRS</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,4010,27904,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8309804$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3553411$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>USHIKI, Tatsuo</creatorcontrib><creatorcontrib>YUI, Ryogo</creatorcontrib><creatorcontrib>FUJITA, Tsuneo</creatorcontrib><title>Immunohistochemical Localization of Serotonin in the Third Ventricular Wall of the Lamprey, Using Backscattered Electron Imaging</title><title>Journal of electron microscopy</title><addtitle>J Electron Microsc (Tokyo)</addtitle><description>The distribution and fine surface structures of serotonin-immunoreactive cells in the third ventricular wall of the lamprey, Lampetra japonica, were examined by backscattered electron (BSE) imaging of immunohistochemical reactions as well as by a secondary emission image of conventional scanning electron microscopy (SEM). The BSE image of immunohistochemical specimens clearly visualized the immunostained structures as bright spots which corresponded to the bulbous terminals of the cerebrospinal fluid (CSF)-contacting neurons. These terminals were grouped in the hypothalamic area on the border of the ventral thalamus caudal to the level of the postoptic commissure. Secondary emission images of conventionally prepared specimens revealed a well-developed labyrinthine structure of nerve terminals and fibers in the corresponding area. The location, surface structure and serotonin content in the nerves showed a similarity between this region of the lamprey and the paraventricular organ of other higher vertebrate classes.</description><subject>Animals</subject><subject>backscattered electron image</subject><subject>Biological and medical sciences</subject><subject>Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges</subject><subject>Cerebral Ventricles - analysis</subject><subject>Cerebral Ventricles - ultrastructure</subject><subject>cerebrospinal fluid (CSF)-contacting neurons</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Image Enhancement - methods</subject><subject>Immunoenzyme Techniques</subject><subject>Lampetra japonica</subject><subject>Lampreys</subject><subject>Male</subject><subject>Microscopy, Electron, Scanning - methods</subject><subject>Neurons - analysis</subject><subject>Neurons - ultrastructure</subject><subject>paraventricular organ</subject><subject>Serotonin - analysis</subject><subject>serotonin-immunoreactivity</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0022-0744</issn><issn>1477-9986</issn><issn>1477-9986</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkU9v0zAYxi0EGmXwEZB8AE6ks2MnTg4cxjS2SpHQRAcTF8tx3qzuErvYjrRx4qPPVaNKSJZ9eH7vHz8PQh8pWVJSszP32Dvfbd3krRrCcjsa7d1SkYIUJX2BFpQLkdV1Vb5EC0LyPCOC89foTQhbQqjglJygE1YUjFO6QP9W4zhZtzEhOr2B1EwNuHHpNn9VNM5i1-Mf4F101licTtwAXm-M7_BPsNEbPQ3K419qGPboXm3UuPPw9BnfBmPv8VelH4JWMYKHDl8OoKNPfVejuk_yW_SqT_-Ad_N7im6_Xa4vrrPm-9Xq4rzJdF6KmAGQtHLbdb0iIArW50zoKu85p61gJa-oaCvG27xmQIQq256QrstpXXQtsJayU_Tp0Hfn3Z8JQpSjCRqGQVlwU5BCsFzwcg9-OYDJ1hA89HLnzaj8k6RE7hOQ_ycgDwnIOYFU_34eNLUjdMfq2fKkf5h1lVwZeq-sNuGIVYzUFeEJyw5YSgYej7LyD7IUTBTy-u63bG7K4k6kvdbsGQG_qFE</recordid><startdate>1986</startdate><enddate>1986</enddate><creator>USHIKI, Tatsuo</creator><creator>YUI, Ryogo</creator><creator>FUJITA, Tsuneo</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1986</creationdate><title>Immunohistochemical Localization of Serotonin in the Third Ventricular Wall of the Lamprey, Using Backscattered Electron Imaging</title><author>USHIKI, Tatsuo ; YUI, Ryogo ; FUJITA, Tsuneo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c267t-ee0553bddfa0e753f237c82f441b7364817b834b293e07a6bf00dd2195dbe3b13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Animals</topic><topic>backscattered electron image</topic><topic>Biological and medical sciences</topic><topic>Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges</topic><topic>Cerebral Ventricles - analysis</topic><topic>Cerebral Ventricles - ultrastructure</topic><topic>cerebrospinal fluid (CSF)-contacting neurons</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Image Enhancement - methods</topic><topic>Immunoenzyme Techniques</topic><topic>Lampetra japonica</topic><topic>Lampreys</topic><topic>Male</topic><topic>Microscopy, Electron, Scanning - methods</topic><topic>Neurons - analysis</topic><topic>Neurons - ultrastructure</topic><topic>paraventricular organ</topic><topic>Serotonin - analysis</topic><topic>serotonin-immunoreactivity</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>online_resources</toplevel><creatorcontrib>USHIKI, Tatsuo</creatorcontrib><creatorcontrib>YUI, Ryogo</creatorcontrib><creatorcontrib>FUJITA, Tsuneo</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of electron microscopy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>USHIKI, Tatsuo</au><au>YUI, Ryogo</au><au>FUJITA, Tsuneo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunohistochemical Localization of Serotonin in the Third Ventricular Wall of the Lamprey, Using Backscattered Electron Imaging</atitle><jtitle>Journal of electron microscopy</jtitle><addtitle>J Electron Microsc (Tokyo)</addtitle><date>1986</date><risdate>1986</risdate><volume>35</volume><issue>2</issue><spage>157</spage><epage>164</epage><pages>157-164</pages><issn>0022-0744</issn><issn>1477-9986</issn><eissn>1477-9986</eissn><coden>JELJA7</coden><abstract>The distribution and fine surface structures of serotonin-immunoreactive cells in the third ventricular wall of the lamprey, Lampetra japonica, were examined by backscattered electron (BSE) imaging of immunohistochemical reactions as well as by a secondary emission image of conventional scanning electron microscopy (SEM). The BSE image of immunohistochemical specimens clearly visualized the immunostained structures as bright spots which corresponded to the bulbous terminals of the cerebrospinal fluid (CSF)-contacting neurons. These terminals were grouped in the hypothalamic area on the border of the ventral thalamus caudal to the level of the postoptic commissure. Secondary emission images of conventionally prepared specimens revealed a well-developed labyrinthine structure of nerve terminals and fibers in the corresponding area. The location, surface structure and serotonin content in the nerves showed a similarity between this region of the lamprey and the paraventricular organ of other higher vertebrate classes.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>3553411</pmid><doi>10.1093/oxfordjournals.jmicro.a050561</doi><tpages>8</tpages></addata></record> |
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| ispartof | Journal of electron microscopy, 1986, Vol.35 (2), p.157-164 |
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| source | MEDLINE; Oxford University Press Journals Digital Archive Legacy |
| subjects | Animals backscattered electron image Biological and medical sciences Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges Cerebral Ventricles - analysis Cerebral Ventricles - ultrastructure cerebrospinal fluid (CSF)-contacting neurons Female Fundamental and applied biological sciences. Psychology Image Enhancement - methods Immunoenzyme Techniques Lampetra japonica Lampreys Male Microscopy, Electron, Scanning - methods Neurons - analysis Neurons - ultrastructure paraventricular organ Serotonin - analysis serotonin-immunoreactivity Vertebrates: nervous system and sense organs |
| title | Immunohistochemical Localization of Serotonin in the Third Ventricular Wall of the Lamprey, Using Backscattered Electron Imaging |
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