A Homogeneous Immunofluorescence Assay Based on Dye-Sensitized Photobleaching

A novel homogeneous immunoassay requiring only one incubation step, and applicable in principle to the determination of low- as well as high-molecular-weight substances, has been developed. The method is based on (a) photooxidation by singlet oxygen ( 1O 2) of a fluorescent substrate (1,3-diphenylis...

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Veröffentlicht in:	Analytical biochemistry 1995-02, Vol.225 (1), p.127-134
Hauptverfasser:	Bystryak, S., Goldiner, I., Niv, A., Nasser, A.M., Goldstein, L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies Antigen-Antibody Reactions Benzofurans Erythrosine Fluorescent Antibody Technique Goats - immunology Immunoglobulin G Indicators and Reagents Kinetics Liposomes Molecular Weight Oxidation-Reduction Oxygen Photolysis Rabbits - immunology Singlet Oxygen
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