Cumulus cell function during bovine oocyte maturation, fertilization, and embryo development in vitro
Several contemporary micromanipulation techniques, such as sperm microinjection, nuclear transfer, and gene transfer by pronuclear injection, require removal of cumulus cells from oocytes or zygotes at various stages. In humans, the cumulus cells are often removed after 15–18 hr of sperm‐oocyte coin...
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| Veröffentlicht in: | Molecular reproduction and development 1995-03, Vol.40 (3), p.338-344 |
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| creator | Zhang, L. (Louisiana State University, Baton Rouge, LA.) Jiang, S Wozniak, P.J Yang, X.Z Godke, R.A |
| description | Several contemporary micromanipulation techniques, such as sperm microinjection, nuclear transfer, and gene transfer by pronuclear injection, require removal of cumulus cells from oocytes or zygotes at various stages. In humans, the cumulus cells are often removed after 15–18 hr of sperm‐oocyte coincubation to assist the identification of the fertilization status. This study was designed to evaluate the function of cumulus cells during oocyte maturation, fertilization, and in vitro development in cattle. Cumulus cells were removed before and after maturation and after fertilization for 0,7,20, and 48 hr. The cumulus‐free oocytes or embryos were cultured either alone or on cumulus cell monolayers prepared on the day of maturation culture. Percentages of oocyte maturation, fertilization, and development to cleavage, morula, and blastocyst stages and to expanding or hatched blastocysts were recorded for statistical analysis by categorical data modeling (CATMOD) procedures.
Cumulus cells removed before maturation significantly reduced the rate of oocyte maturation (4–26% vs. 93–96%), fertilization (0–9% vs. 91–92%), and in vitro development at all stages evaluated. Cumulus cells removed immediately prior to in vitro fertilization (IVF) or 7 hr after IVF reduced the rates of fertilization (58–60% and 71%, respectively, vs. 91–92% for controls), cleavage development (40–47% and 53–54% vs. 74–78% for controls), and morula plus blastocyst development (15% and 24% vs. 45%, P < 0.05). Cumulus cell co‐culture started at various stages had no effect on fertilization and cleavage development but significantly improved rates of embryo development to morula or blastocyst stages (P < 0.05). Cumulus cell removal at 20 hr after IVF resulted in similar development to controls (P > 0.05) at all stages tested in this study. The intact state of surrounding cumulus cells of oocytes or embryos appears to be beneficial before or shortly after insemination (at or before 7 hr of IVF) but not essential at 20 hr after IVF. © 1995 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/mrd.1080400310 |
| format | Article |
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Cumulus cells removed before maturation significantly reduced the rate of oocyte maturation (4–26% vs. 93–96%), fertilization (0–9% vs. 91–92%), and in vitro development at all stages evaluated. Cumulus cells removed immediately prior to in vitro fertilization (IVF) or 7 hr after IVF reduced the rates of fertilization (58–60% and 71%, respectively, vs. 91–92% for controls), cleavage development (40–47% and 53–54% vs. 74–78% for controls), and morula plus blastocyst development (15% and 24% vs. 45%, P < 0.05). Cumulus cell co‐culture started at various stages had no effect on fertilization and cleavage development but significantly improved rates of embryo development to morula or blastocyst stages (P < 0.05). Cumulus cell removal at 20 hr after IVF resulted in similar development to controls (P > 0.05) at all stages tested in this study. The intact state of surrounding cumulus cells of oocytes or embryos appears to be beneficial before or shortly after insemination (at or before 7 hr of IVF) but not essential at 20 hr after IVF. © 1995 Wiley‐Liss, Inc.</description><identifier>ISSN: 1040-452X</identifier><identifier>EISSN: 1098-2795</identifier><identifier>DOI: 10.1002/mrd.1080400310</identifier><identifier>PMID: 7772344</identifier><identifier>CODEN: MREDEE</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>ANIMAL EMBRYOS ; Animals ; Biological and medical sciences ; BLASTOCYST ; Blastocyst - cytology ; Cattle ; CELL DIVISION ; CIGOTOS ; CLEAVAGE ; COWS ; CULTIVO DE EMBRIONES ; CULTIVO IN VITRO ; CULTURE D'EMBRYON ; CULTURE IN VITRO ; CUMULUS OOPHORUS ; Cumulus-oocyte complexes ; DESARROLLO EMBRIONARIO ; DEVELOPPEMENT EMBRYONNAIRE ; DIVISION CELLULAIRE ; DIVISION CELULAR ; EMBRIONES ANIMALES ; EMBRYO CULTURE ; EMBRYON ANIMAL ; Embryonic and Fetal Development ; EMBRYONIC DEVELOPMENT ; FECONDATION ; FECUNDACION ; Female ; FERTILIZATION ; Fertilization in Vitro ; Fundamental and applied biological sciences. Psychology ; Gap junctions ; IN VITRO CULTURE ; In Vitro Techniques ; MADURACION ; Mammalian female genital system ; MATURATION ; Morphology. Physiology ; MORULA ; Oocytes - cytology ; Oocytes - growth & development ; Oogenesis ; OVA ; OVULE ; OVULO ; Time Factors ; VACA ; VACHE ; Vertebrates: reproduction ; Zona pellucida ; ZYGOTE ; ZYGOTES</subject><ispartof>Molecular reproduction and development, 1995-03, Vol.40 (3), p.338-344</ispartof><rights>Copyright © 1995 Wiley‐Liss, Inc.</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4920-4b609c15eaab602bf8b8fe41b18629729b2bfa54fc4bb51a782704140c52d4d33</citedby><cites>FETCH-LOGICAL-c4920-4b609c15eaab602bf8b8fe41b18629729b2bfa54fc4bb51a782704140c52d4d33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fmrd.1080400310$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fmrd.1080400310$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3450169$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7772344$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, L. (Louisiana State University, Baton Rouge, LA.)</creatorcontrib><creatorcontrib>Jiang, S</creatorcontrib><creatorcontrib>Wozniak, P.J</creatorcontrib><creatorcontrib>Yang, X.Z</creatorcontrib><creatorcontrib>Godke, R.A</creatorcontrib><title>Cumulus cell function during bovine oocyte maturation, fertilization, and embryo development in vitro</title><title>Molecular reproduction and development</title><addtitle>Mol. Reprod. Dev</addtitle><description>Several contemporary micromanipulation techniques, such as sperm microinjection, nuclear transfer, and gene transfer by pronuclear injection, require removal of cumulus cells from oocytes or zygotes at various stages. In humans, the cumulus cells are often removed after 15–18 hr of sperm‐oocyte coincubation to assist the identification of the fertilization status. This study was designed to evaluate the function of cumulus cells during oocyte maturation, fertilization, and in vitro development in cattle. Cumulus cells were removed before and after maturation and after fertilization for 0,7,20, and 48 hr. The cumulus‐free oocytes or embryos were cultured either alone or on cumulus cell monolayers prepared on the day of maturation culture. Percentages of oocyte maturation, fertilization, and development to cleavage, morula, and blastocyst stages and to expanding or hatched blastocysts were recorded for statistical analysis by categorical data modeling (CATMOD) procedures.
Cumulus cells removed before maturation significantly reduced the rate of oocyte maturation (4–26% vs. 93–96%), fertilization (0–9% vs. 91–92%), and in vitro development at all stages evaluated. Cumulus cells removed immediately prior to in vitro fertilization (IVF) or 7 hr after IVF reduced the rates of fertilization (58–60% and 71%, respectively, vs. 91–92% for controls), cleavage development (40–47% and 53–54% vs. 74–78% for controls), and morula plus blastocyst development (15% and 24% vs. 45%, P < 0.05). Cumulus cell co‐culture started at various stages had no effect on fertilization and cleavage development but significantly improved rates of embryo development to morula or blastocyst stages (P < 0.05). Cumulus cell removal at 20 hr after IVF resulted in similar development to controls (P > 0.05) at all stages tested in this study. The intact state of surrounding cumulus cells of oocytes or embryos appears to be beneficial before or shortly after insemination (at or before 7 hr of IVF) but not essential at 20 hr after IVF. © 1995 Wiley‐Liss, Inc.</description><subject>ANIMAL EMBRYOS</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>BLASTOCYST</subject><subject>Blastocyst - cytology</subject><subject>Cattle</subject><subject>CELL DIVISION</subject><subject>CIGOTOS</subject><subject>CLEAVAGE</subject><subject>COWS</subject><subject>CULTIVO DE EMBRIONES</subject><subject>CULTIVO IN VITRO</subject><subject>CULTURE D'EMBRYON</subject><subject>CULTURE IN VITRO</subject><subject>CUMULUS OOPHORUS</subject><subject>Cumulus-oocyte complexes</subject><subject>DESARROLLO EMBRIONARIO</subject><subject>DEVELOPPEMENT EMBRYONNAIRE</subject><subject>DIVISION CELLULAIRE</subject><subject>DIVISION CELULAR</subject><subject>EMBRIONES ANIMALES</subject><subject>EMBRYO CULTURE</subject><subject>EMBRYON ANIMAL</subject><subject>Embryonic and Fetal Development</subject><subject>EMBRYONIC DEVELOPMENT</subject><subject>FECONDATION</subject><subject>FECUNDACION</subject><subject>Female</subject><subject>FERTILIZATION</subject><subject>Fertilization in Vitro</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gap junctions</subject><subject>IN VITRO CULTURE</subject><subject>In Vitro Techniques</subject><subject>MADURACION</subject><subject>Mammalian female genital system</subject><subject>MATURATION</subject><subject>Morphology. Physiology</subject><subject>MORULA</subject><subject>Oocytes - cytology</subject><subject>Oocytes - growth & development</subject><subject>Oogenesis</subject><subject>OVA</subject><subject>OVULE</subject><subject>OVULO</subject><subject>Time Factors</subject><subject>VACA</subject><subject>VACHE</subject><subject>Vertebrates: reproduction</subject><subject>Zona pellucida</subject><subject>ZYGOTE</subject><subject>ZYGOTES</subject><issn>1040-452X</issn><issn>1098-2795</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1v1DAQxSNEVUrhygEJyQfEiRR_xvGxWmBbqYAElEa9WLZjV4YkXuxkYfnr61WiRZw4eez3ezPjVxTPEDxDEOI3fWxzUUMKIUHwQXGCoKhLzAV7uK8pLCnDzaPicUrfIYRC1PC4OOacY0LpSWFXUz91UwLGdh1w02BGHwbQTtEPd0CHrR8sCMHsRgt6NU5R7fXXwNk4-s7_Wa5qaIHtddwF0Nqt7cKmt8MI_AC2fozhSXHkVJfs0-U8La7fv_u6uiivPq0vV-dXpaEC5011BYVBzCqVK6xdrWtnKdKorrDgWOj8phh1hmrNkOI15pAiCg3DLW0JOS1ezX03MfycbBpl79P-Z2qwYUqSc4IxqXAGz2bQxJBStE5uou9V3EkE5T5XmXOVf3PNhhdL50n3tj3gS5BZf7noKhnVuagG49MBI5RBVImMiRn75Tu7-89Q-eHz239WKGevT6P9ffCq-ENWnHAmbz6uZXPTrG-_3TayyfzzmXcqSHUX8zrXXwSrKoIFuQfmsKn9</recordid><startdate>199503</startdate><enddate>199503</enddate><creator>Zhang, L. (Louisiana State University, Baton Rouge, LA.)</creator><creator>Jiang, S</creator><creator>Wozniak, P.J</creator><creator>Yang, X.Z</creator><creator>Godke, R.A</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199503</creationdate><title>Cumulus cell function during bovine oocyte maturation, fertilization, and embryo development in vitro</title><author>Zhang, L. (Louisiana State University, Baton Rouge, LA.) ; Jiang, S ; Wozniak, P.J ; Yang, X.Z ; Godke, R.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4920-4b609c15eaab602bf8b8fe41b18629729b2bfa54fc4bb51a782704140c52d4d33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>ANIMAL EMBRYOS</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>BLASTOCYST</topic><topic>Blastocyst - cytology</topic><topic>Cattle</topic><topic>CELL DIVISION</topic><topic>CIGOTOS</topic><topic>CLEAVAGE</topic><topic>COWS</topic><topic>CULTIVO DE EMBRIONES</topic><topic>CULTIVO IN VITRO</topic><topic>CULTURE D'EMBRYON</topic><topic>CULTURE IN VITRO</topic><topic>CUMULUS OOPHORUS</topic><topic>Cumulus-oocyte complexes</topic><topic>DESARROLLO EMBRIONARIO</topic><topic>DEVELOPPEMENT EMBRYONNAIRE</topic><topic>DIVISION CELLULAIRE</topic><topic>DIVISION CELULAR</topic><topic>EMBRIONES ANIMALES</topic><topic>EMBRYO CULTURE</topic><topic>EMBRYON ANIMAL</topic><topic>Embryonic and Fetal Development</topic><topic>EMBRYONIC DEVELOPMENT</topic><topic>FECONDATION</topic><topic>FECUNDACION</topic><topic>Female</topic><topic>FERTILIZATION</topic><topic>Fertilization in Vitro</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gap junctions</topic><topic>IN VITRO CULTURE</topic><topic>In Vitro Techniques</topic><topic>MADURACION</topic><topic>Mammalian female genital system</topic><topic>MATURATION</topic><topic>Morphology. Physiology</topic><topic>MORULA</topic><topic>Oocytes - cytology</topic><topic>Oocytes - growth & development</topic><topic>Oogenesis</topic><topic>OVA</topic><topic>OVULE</topic><topic>OVULO</topic><topic>Time Factors</topic><topic>VACA</topic><topic>VACHE</topic><topic>Vertebrates: reproduction</topic><topic>Zona pellucida</topic><topic>ZYGOTE</topic><topic>ZYGOTES</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, L. (Louisiana State University, Baton Rouge, LA.)</creatorcontrib><creatorcontrib>Jiang, S</creatorcontrib><creatorcontrib>Wozniak, P.J</creatorcontrib><creatorcontrib>Yang, X.Z</creatorcontrib><creatorcontrib>Godke, R.A</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular reproduction and development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, L. (Louisiana State University, Baton Rouge, LA.)</au><au>Jiang, S</au><au>Wozniak, P.J</au><au>Yang, X.Z</au><au>Godke, R.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cumulus cell function during bovine oocyte maturation, fertilization, and embryo development in vitro</atitle><jtitle>Molecular reproduction and development</jtitle><addtitle>Mol. Reprod. Dev</addtitle><date>1995-03</date><risdate>1995</risdate><volume>40</volume><issue>3</issue><spage>338</spage><epage>344</epage><pages>338-344</pages><issn>1040-452X</issn><eissn>1098-2795</eissn><coden>MREDEE</coden><abstract>Several contemporary micromanipulation techniques, such as sperm microinjection, nuclear transfer, and gene transfer by pronuclear injection, require removal of cumulus cells from oocytes or zygotes at various stages. In humans, the cumulus cells are often removed after 15–18 hr of sperm‐oocyte coincubation to assist the identification of the fertilization status. This study was designed to evaluate the function of cumulus cells during oocyte maturation, fertilization, and in vitro development in cattle. Cumulus cells were removed before and after maturation and after fertilization for 0,7,20, and 48 hr. The cumulus‐free oocytes or embryos were cultured either alone or on cumulus cell monolayers prepared on the day of maturation culture. Percentages of oocyte maturation, fertilization, and development to cleavage, morula, and blastocyst stages and to expanding or hatched blastocysts were recorded for statistical analysis by categorical data modeling (CATMOD) procedures.
Cumulus cells removed before maturation significantly reduced the rate of oocyte maturation (4–26% vs. 93–96%), fertilization (0–9% vs. 91–92%), and in vitro development at all stages evaluated. Cumulus cells removed immediately prior to in vitro fertilization (IVF) or 7 hr after IVF reduced the rates of fertilization (58–60% and 71%, respectively, vs. 91–92% for controls), cleavage development (40–47% and 53–54% vs. 74–78% for controls), and morula plus blastocyst development (15% and 24% vs. 45%, P < 0.05). Cumulus cell co‐culture started at various stages had no effect on fertilization and cleavage development but significantly improved rates of embryo development to morula or blastocyst stages (P < 0.05). Cumulus cell removal at 20 hr after IVF resulted in similar development to controls (P > 0.05) at all stages tested in this study. The intact state of surrounding cumulus cells of oocytes or embryos appears to be beneficial before or shortly after insemination (at or before 7 hr of IVF) but not essential at 20 hr after IVF. © 1995 Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>7772344</pmid><doi>10.1002/mrd.1080400310</doi><tpages>7</tpages></addata></record> |
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| ispartof | Molecular reproduction and development, 1995-03, Vol.40 (3), p.338-344 |
| issn | 1040-452X 1098-2795 |
| language | eng |
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| subjects | ANIMAL EMBRYOS Animals Biological and medical sciences BLASTOCYST Blastocyst - cytology Cattle CELL DIVISION CIGOTOS CLEAVAGE COWS CULTIVO DE EMBRIONES CULTIVO IN VITRO CULTURE D'EMBRYON CULTURE IN VITRO CUMULUS OOPHORUS Cumulus-oocyte complexes DESARROLLO EMBRIONARIO DEVELOPPEMENT EMBRYONNAIRE DIVISION CELLULAIRE DIVISION CELULAR EMBRIONES ANIMALES EMBRYO CULTURE EMBRYON ANIMAL Embryonic and Fetal Development EMBRYONIC DEVELOPMENT FECONDATION FECUNDACION Female FERTILIZATION Fertilization in Vitro Fundamental and applied biological sciences. Psychology Gap junctions IN VITRO CULTURE In Vitro Techniques MADURACION Mammalian female genital system MATURATION Morphology. Physiology MORULA Oocytes - cytology Oocytes - growth & development Oogenesis OVA OVULE OVULO Time Factors VACA VACHE Vertebrates: reproduction Zona pellucida ZYGOTE ZYGOTES |
| title | Cumulus cell function during bovine oocyte maturation, fertilization, and embryo development in vitro |
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