Fusion of pro region of subtilisin to staphylococcal protein A and its secretion by Bacillus subtilis

Subtilisin is synthesized as a preproenzyme in Bacillus subtilis. We fused that region of the subtilisin gene, ( apr[BamP]), which encodes the signal sequence and pro region, to the mature gene sequence ( spa) for a heterologous protein (staphylococcal protein A). B. subtilis cells harboring this ge...

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Veröffentlicht in:	Gene 1986, Vol.49 (1), p.23-28
Hauptverfasser:	Vasantha, N., Thompson, Leo D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Bacillus subtilis Bacillus subtilis - enzymology Bacillus subtilis - genetics Base Sequence Biological and medical sciences Biotechnology cloning Enzyme Precursors - genetics Escherichia coli - genetics Fundamental and applied biological sciences. Psychology gene expression gene fusion Genes Genes, Bacterial Genetic engineering Genetic technics Methods. Procedures. Technologies plasmid prepropeptide preprosubtilisin Recombinant DNA signal sequence Staphylococcal Protein A - genetics subtilisin Subtilisins - genetics transformation Vectors (cloning, transfer, expression). Insertion sequences and transposons
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Beschreibung
Zusammenfassung:	Subtilisin is synthesized as a preproenzyme in Bacillus subtilis. We fused that region of the subtilisin gene, ( apr[BamP]), which encodes the signal sequence and pro region, to the mature gene sequence ( spa) for a heterologous protein (staphylococcal protein A). B. subtilis cells harboring this gene fusion synthesized a fusion protein consisting of the signal and pro sequence of subtilisin fused to the protein A; the signal sequence was processed and a fusion protein (pro + protein A) was secreted into the growth medium.
ISSN:	0378-1119 1879-0038
DOI:	10.1016/0378-1119(86)90382-3