The hypervariable region in the genes coding for entomopathogenic crystal proteins of Bacillus thuringiensis: nucleotide sequence of the kurhd1 gene of subsp. kurstaki HD1
One of the genes for the entomophatogenic crystal protein of Bacillus thuringiensis (subsp. kurstaki strain HD1) has been cloned in Escherichia coli, and its nucleotide sequence determined completely. The gene is contained within a 4360-bp-long HpaI- PstI DNA restriction fragment and codes for a pol...
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| Veröffentlicht in: | Gene 1986, Vol.48 (1), p.109-118 |
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| creator | Geiser, Martin Schweitzer, Silvia Grimm, Charlotte |
| description | One of the genes for the entomophatogenic crystal protein of
Bacillus thuringiensis (subsp.
kurstaki strain HD1) has been cloned in
Escherichia coli, and its nucleotide sequence determined completely. The gene is contained within a 4360-bp-long
HpaI-
PstI DNA restriction fragment and codes for a polypeptide of 1155 amino acid residues. The protoxin protein has a predicted
M
r
of 130625. The
E. coli-deriveSd protoxin gene product is biologically active against
Heliothis virescens larvae in a biotest assay. Extensive computer comparisons with other published
B. thuringiensis subsp.
kurstaki strains HD1, HD73, and
B. thuringiensis subsp.
sotto gene sequences reveal hypervariable regions in the first half of the protoxin coding sequence. These regions are responsible for the biological activity of the protein product of the cloned gene, and may explain the different biological activities of these different protoxins. |
| doi_str_mv | 10.1016/0378-1119(86)90357-4 |
| format | Article |
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Bacillus thuringiensis (subsp.
kurstaki strain HD1) has been cloned in
Escherichia coli, and its nucleotide sequence determined completely. The gene is contained within a 4360-bp-long
HpaI-
PstI DNA restriction fragment and codes for a polypeptide of 1155 amino acid residues. The protoxin protein has a predicted
M
r
of 130625. The
E. coli-deriveSd protoxin gene product is biologically active against
Heliothis virescens larvae in a biotest assay. Extensive computer comparisons with other published
B. thuringiensis subsp.
kurstaki strains HD1, HD73, and
B. thuringiensis subsp.
sotto gene sequences reveal hypervariable regions in the first half of the protoxin coding sequence. These regions are responsible for the biological activity of the protein product of the cloned gene, and may explain the different biological activities of these different protoxins.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/0378-1119(86)90357-4</identifier><identifier>PMID: 3557124</identifier><identifier>CODEN: GENED6</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>Bacillus thuringiensis ; Bacillus thuringiensis - genetics ; Bacillus thuringiensis kurstaki ; Bacterial Proteins - genetics ; Bacterial Toxins - genetics ; Bacteriology ; Base Sequence ; Biological and medical sciences ; Biotechnology ; cloning ; Cloning, Molecular ; DNA, Bacterial - genetics ; E coli host ; Endotoxins ; Escherichia coli ; Fundamental and applied biological sciences. Psychology ; gene expression ; genes ; Genes, Bacterial ; Genetic engineering ; Genetic technics ; Genetics ; Hemolysin Proteins ; Methods. Procedures. Technologies ; Microbiology ; nucleotide sequence ; plasmid DNA ; Protein Precursors - genetics ; proteins ; protoxin ; Recombinant DNA ; spore crystals ; Synthetic digonucleotides and genes. Sequencing ; toxin gene</subject><ispartof>Gene, 1986, Vol.48 (1), p.109-118</ispartof><rights>1986</rights><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c483t-d7e660ec52ed2e51442ae06a0987cb65be3379b6fa125560e4407cb23b807243</citedby><cites>FETCH-LOGICAL-c483t-d7e660ec52ed2e51442ae06a0987cb65be3379b6fa125560e4407cb23b807243</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0378-1119(86)90357-4$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,782,786,3554,4028,27932,27933,27934,46004</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8283256$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3557124$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Geiser, Martin</creatorcontrib><creatorcontrib>Schweitzer, Silvia</creatorcontrib><creatorcontrib>Grimm, Charlotte</creatorcontrib><title>The hypervariable region in the genes coding for entomopathogenic crystal proteins of Bacillus thuringiensis: nucleotide sequence of the kurhd1 gene of subsp. kurstaki HD1</title><title>Gene</title><addtitle>Gene</addtitle><description>One of the genes for the entomophatogenic crystal protein of
Bacillus thuringiensis (subsp.
kurstaki strain HD1) has been cloned in
Escherichia coli, and its nucleotide sequence determined completely. The gene is contained within a 4360-bp-long
HpaI-
PstI DNA restriction fragment and codes for a polypeptide of 1155 amino acid residues. The protoxin protein has a predicted
M
r
of 130625. The
E. coli-deriveSd protoxin gene product is biologically active against
Heliothis virescens larvae in a biotest assay. Extensive computer comparisons with other published
B. thuringiensis subsp.
kurstaki strains HD1, HD73, and
B. thuringiensis subsp.
sotto gene sequences reveal hypervariable regions in the first half of the protoxin coding sequence. These regions are responsible for the biological activity of the protein product of the cloned gene, and may explain the different biological activities of these different protoxins.</description><subject>Bacillus thuringiensis</subject><subject>Bacillus thuringiensis - genetics</subject><subject>Bacillus thuringiensis kurstaki</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Toxins - genetics</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>cloning</subject><subject>Cloning, Molecular</subject><subject>DNA, Bacterial - genetics</subject><subject>E coli host</subject><subject>Endotoxins</subject><subject>Escherichia coli</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene expression</subject><subject>genes</subject><subject>Genes, Bacterial</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Genetics</subject><subject>Hemolysin Proteins</subject><subject>Methods. Procedures. Technologies</subject><subject>Microbiology</subject><subject>nucleotide sequence</subject><subject>plasmid DNA</subject><subject>Protein Precursors - genetics</subject><subject>proteins</subject><subject>protoxin</subject><subject>Recombinant DNA</subject><subject>spore crystals</subject><subject>Synthetic digonucleotides and genes. Sequencing</subject><subject>toxin gene</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAUhS0EKtOBNwDJC4RgkeLf2GGBBC1QpEpsZm85zs2MacYOdlJpnomXxOmMZgneWPL57rnX9yD0ipIrSmj9gXClK0pp807X7xvCparEE7SiWjUVIVw_Rasz8hxd5vyLlCMlu0AXXEpFmVihP5sd4N1hhPRgk7ftADjB1seAfcBT0bYQIGMXOx-2uI8JQ5jiPo522sWieYddOuTJDnhMcQIfMo49_mKdH4Y5F4s5lUoPIfv8EYfZDRAn3wHO8HuG4GDBl0b3c9p19LHf8pTnNo9Xy2sxv_f49oa-QM96O2R4ebrXaPPt6-b6trr7-f3H9ee7ygnNp6pTUNcEnGTQMZBUCGaB1JY0Wrm2li1wrpq27i1lUhZSCFIExltNFBN8jd4ebcuHyoh5MnufHQyDDRDnbJTiZaXy_yAVinJW8DUSR9ClmHOC3ozJ7206GErMkqVZgjJLUEbX5jFLs_i_PvnP7R66c9EpvKK_Oek2Ozv0yQbn8xnTTHMm64J9OmJQdvbgIZns_LL5zidwk-mi__ccfwG8eb0u</recordid><startdate>1986</startdate><enddate>1986</enddate><creator>Geiser, Martin</creator><creator>Schweitzer, Silvia</creator><creator>Grimm, Charlotte</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>1986</creationdate><title>The hypervariable region in the genes coding for entomopathogenic crystal proteins of Bacillus thuringiensis: nucleotide sequence of the kurhd1 gene of subsp. kurstaki HD1</title><author>Geiser, Martin ; Schweitzer, Silvia ; Grimm, Charlotte</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c483t-d7e660ec52ed2e51442ae06a0987cb65be3379b6fa125560e4407cb23b807243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Bacillus thuringiensis</topic><topic>Bacillus thuringiensis - genetics</topic><topic>Bacillus thuringiensis kurstaki</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Toxins - genetics</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>cloning</topic><topic>Cloning, Molecular</topic><topic>DNA, Bacterial - genetics</topic><topic>E coli host</topic><topic>Endotoxins</topic><topic>Escherichia coli</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene expression</topic><topic>genes</topic><topic>Genes, Bacterial</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>Genetics</topic><topic>Hemolysin Proteins</topic><topic>Methods. Procedures. Technologies</topic><topic>Microbiology</topic><topic>nucleotide sequence</topic><topic>plasmid DNA</topic><topic>Protein Precursors - genetics</topic><topic>proteins</topic><topic>protoxin</topic><topic>Recombinant DNA</topic><topic>spore crystals</topic><topic>Synthetic digonucleotides and genes. Sequencing</topic><topic>toxin gene</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Geiser, Martin</creatorcontrib><creatorcontrib>Schweitzer, Silvia</creatorcontrib><creatorcontrib>Grimm, Charlotte</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Geiser, Martin</au><au>Schweitzer, Silvia</au><au>Grimm, Charlotte</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The hypervariable region in the genes coding for entomopathogenic crystal proteins of Bacillus thuringiensis: nucleotide sequence of the kurhd1 gene of subsp. kurstaki HD1</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1986</date><risdate>1986</risdate><volume>48</volume><issue>1</issue><spage>109</spage><epage>118</epage><pages>109-118</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><coden>GENED6</coden><abstract>One of the genes for the entomophatogenic crystal protein of
Bacillus thuringiensis (subsp.
kurstaki strain HD1) has been cloned in
Escherichia coli, and its nucleotide sequence determined completely. The gene is contained within a 4360-bp-long
HpaI-
PstI DNA restriction fragment and codes for a polypeptide of 1155 amino acid residues. The protoxin protein has a predicted
M
r
of 130625. The
E. coli-deriveSd protoxin gene product is biologically active against
Heliothis virescens larvae in a biotest assay. Extensive computer comparisons with other published
B. thuringiensis subsp.
kurstaki strains HD1, HD73, and
B. thuringiensis subsp.
sotto gene sequences reveal hypervariable regions in the first half of the protoxin coding sequence. These regions are responsible for the biological activity of the protein product of the cloned gene, and may explain the different biological activities of these different protoxins.</abstract><cop>Lausanne</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>3557124</pmid><doi>10.1016/0378-1119(86)90357-4</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Gene, 1986, Vol.48 (1), p.109-118 |
| issn | 0378-1119 1879-0038 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77311154 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Bacillus thuringiensis Bacillus thuringiensis - genetics Bacillus thuringiensis kurstaki Bacterial Proteins - genetics Bacterial Toxins - genetics Bacteriology Base Sequence Biological and medical sciences Biotechnology cloning Cloning, Molecular DNA, Bacterial - genetics E coli host Endotoxins Escherichia coli Fundamental and applied biological sciences. Psychology gene expression genes Genes, Bacterial Genetic engineering Genetic technics Genetics Hemolysin Proteins Methods. Procedures. Technologies Microbiology nucleotide sequence plasmid DNA Protein Precursors - genetics proteins protoxin Recombinant DNA spore crystals Synthetic digonucleotides and genes. Sequencing toxin gene |
| title | The hypervariable region in the genes coding for entomopathogenic crystal proteins of Bacillus thuringiensis: nucleotide sequence of the kurhd1 gene of subsp. kurstaki HD1 |
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