Two rapid microscale procedures for isolation of total RNA from leaves rich in polyphenols and polysaccharides: application for sensitive detection of grapevine viroids

Two rapid microscale procedures for isolation of total RNA from grapevine leaves are described. Homogenized leaf tissue is phenol extracted and polyphenols and polysaccharides are removed by either an aqueous two-phase system followed by DEAE-cellulose chromatography or by differential precipitation...

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Veröffentlicht in:	Journal of virological methods 1995-03, Vol.52 (1), p.209-218
Hauptverfasser:	Staub, U., Polivka, H., Gross, H.J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Aqueous two-phase system Base Sequence Biological and medical sciences Chromatography, DEAE-Cellulose Differential solvent precipitation DNA Primers Ethylene Glycols Flavonoids Fruit - virology Fundamental and applied biological sciences. Psychology Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control Grapevine yellow speckle viroid 1 (GYSVd1) Hop stunt viroid grapevine (HSVdg) Microbiology Microchemistry - methods Molecular Sequence Data Phenols Phytopathology. Animal pests. Plant and forest protection Plant Leaves Plant Viruses - genetics Plant Viruses - isolation & purification Plant viruses and viroids Polymerase Chain Reaction - methods Polymers Polyphenols Polysaccharides Reverse transcription polymerase chain reaction (RT-PCR) RNA extraction RNA, Viral - isolation & purification Sensitivity and Specificity Solvents Techniques used in virology Viroids - isolation & purification Virology Vitis
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