ROLE OF CALCIUM ION ON PEPSINOGEN SECRETION BY ORGAN CULTURE OF RABBIT GASTRIC MUCOSA
The role of intracellular and extracellular calcium ion of gastric chief cells for pepsinogen secretion stimulated by carbachol and CCK-8 was evaluated using nicorandil (intracellular calcium antagonist) and verapamil (calcium channel blocker) in organ culture of rabbit gastric mucosa. The pepsinoge...
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| Veröffentlicht in: | Nippon Shokakibyo Gakkai Zasshi 1986, Vol.83(12), pp.2514-2519 |
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| container_issue | 12 |
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| container_title | Nippon Shokakibyo Gakkai Zasshi |
| container_volume | 83 |
| creator | MIYAMOTO, Tadahisa ITO, Makoto MATSUSAKO, Kei IKEDA, Kazuo JOH, Takasi IMAI, Sinpei YASUE, Naoji YOKOYAMA, Yosihumi KATSUMI, Kouhei TAKEUCHI, Toshihiko NOGUCHI, Yosiki |
| description | The role of intracellular and extracellular calcium ion of gastric chief cells for pepsinogen secretion stimulated by carbachol and CCK-8 was evaluated using nicorandil (intracellular calcium antagonist) and verapamil (calcium channel blocker) in organ culture of rabbit gastric mucosa. The pepsinogen secretion stimulated by carbachol (10-4M) was significantly inhibited by 10-5 and 10-4M nicorandil and CCK-8 (10-8M) stimulated pepsinogen secretion was also significantly inhibited by 10-6, 10-5 and 10-4M nicorandil. On the other hand, verapamil (10-6-10-4M) did not inhibit the pepsinogen secretion induced by carbachol and CCK-8. We conclude that a mobilization of the intracellular calcium ion of chief cell, not extracellular one, plays an important role in the mechnisms of pepsinogen secretion stimulated by carbachol and CCK-8. |
| doi_str_mv | 10.11405/nisshoshi1964.83.2514 |
| format | Article |
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The pepsinogen secretion stimulated by carbachol (10-4M) was significantly inhibited by 10-5 and 10-4M nicorandil and CCK-8 (10-8M) stimulated pepsinogen secretion was also significantly inhibited by 10-6, 10-5 and 10-4M nicorandil. On the other hand, verapamil (10-6-10-4M) did not inhibit the pepsinogen secretion induced by carbachol and CCK-8. We conclude that a mobilization of the intracellular calcium ion of chief cell, not extracellular one, plays an important role in the mechnisms of pepsinogen secretion stimulated by carbachol and CCK-8.</description><identifier>ISSN: 0446-6586</identifier><identifier>EISSN: 1349-7693</identifier><identifier>DOI: 10.11405/nisshoshi1964.83.2514</identifier><identifier>PMID: 2951540</identifier><language>jpn</language><publisher>Japan: The Japanese Society of Gastroenterology</publisher><subject>Animals ; Calcium - physiology ; Gastric Mucosa - secretion ; Male ; Niacinamide - analogs & derivatives ; Niacinamide - pharmacology ; Nicorandil ; Organ Culture Techniques ; Pepsinogens - secretion ; Rabbits ; Verapamil - pharmacology</subject><ispartof>Nippon Shokakibyo Gakkai Zasshi, 1986, Vol.83(12), pp.2514-2519</ispartof><rights>The Japanese Society of Gastroenterology</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27907,27908</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2951540$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MIYAMOTO, Tadahisa</creatorcontrib><creatorcontrib>ITO, Makoto</creatorcontrib><creatorcontrib>MATSUSAKO, Kei</creatorcontrib><creatorcontrib>IKEDA, Kazuo</creatorcontrib><creatorcontrib>JOH, Takasi</creatorcontrib><creatorcontrib>IMAI, Sinpei</creatorcontrib><creatorcontrib>YASUE, Naoji</creatorcontrib><creatorcontrib>YOKOYAMA, Yosihumi</creatorcontrib><creatorcontrib>KATSUMI, Kouhei</creatorcontrib><creatorcontrib>TAKEUCHI, Toshihiko</creatorcontrib><creatorcontrib>NOGUCHI, Yosiki</creatorcontrib><title>ROLE OF CALCIUM ION ON PEPSINOGEN SECRETION BY ORGAN CULTURE OF RABBIT GASTRIC MUCOSA</title><title>Nippon Shokakibyo Gakkai Zasshi</title><addtitle>Nippon Shokakibyo Gakkai Zasshi</addtitle><description>The role of intracellular and extracellular calcium ion of gastric chief cells for pepsinogen secretion stimulated by carbachol and CCK-8 was evaluated using nicorandil (intracellular calcium antagonist) and verapamil (calcium channel blocker) in organ culture of rabbit gastric mucosa. The pepsinogen secretion stimulated by carbachol (10-4M) was significantly inhibited by 10-5 and 10-4M nicorandil and CCK-8 (10-8M) stimulated pepsinogen secretion was also significantly inhibited by 10-6, 10-5 and 10-4M nicorandil. On the other hand, verapamil (10-6-10-4M) did not inhibit the pepsinogen secretion induced by carbachol and CCK-8. We conclude that a mobilization of the intracellular calcium ion of chief cell, not extracellular one, plays an important role in the mechnisms of pepsinogen secretion stimulated by carbachol and CCK-8.</description><subject>Animals</subject><subject>Calcium - physiology</subject><subject>Gastric Mucosa - secretion</subject><subject>Male</subject><subject>Niacinamide - analogs & derivatives</subject><subject>Niacinamide - pharmacology</subject><subject>Nicorandil</subject><subject>Organ Culture Techniques</subject><subject>Pepsinogens - secretion</subject><subject>Rabbits</subject><subject>Verapamil - pharmacology</subject><issn>0446-6586</issn><issn>1349-7693</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkFtvgjAUx5tlizPOj7ClT3vDtbSl9BEJOhIEw-VhT03BMjGoG9WHffvhJCbLuSXndy7JH4AXjGYYU8TeDo0x26PZNlg4dOaSmc0wvQNjTKiwuCPIPRgjSh3LYa7zCKbGNCVCSDDhEjICI1swzCgagyJNogAmC-h7kR8WKxgmMex9HayzME6WQQyzwE-D_NKff8AkXXox9IsoL9K_vdSbz8McLr0sT0Mfrgo_ybwn8FCr1ujpUCegWAS5_25FyTLsP1k7zAWyGBNqwxUhDiOUM3ejNCa1i0te90FtgbGqHVyVtsbMVT13S2TXxKFMUa4FmYDX692v7vh91uYk942pdNuqgz6ejeScIMJ6m4DnYfBc7vVGfnXNXnU_ctCh59GV78xJfeobV92pqVot_-ktXSKxPeSL8Lexaqs6qQ_kFwgUdTY</recordid><startdate>198612</startdate><enddate>198612</enddate><creator>MIYAMOTO, Tadahisa</creator><creator>ITO, Makoto</creator><creator>MATSUSAKO, Kei</creator><creator>IKEDA, Kazuo</creator><creator>JOH, Takasi</creator><creator>IMAI, Sinpei</creator><creator>YASUE, Naoji</creator><creator>YOKOYAMA, Yosihumi</creator><creator>KATSUMI, Kouhei</creator><creator>TAKEUCHI, Toshihiko</creator><creator>NOGUCHI, Yosiki</creator><general>The Japanese Society of Gastroenterology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>198612</creationdate><title>ROLE OF CALCIUM ION ON PEPSINOGEN SECRETION BY ORGAN CULTURE OF RABBIT GASTRIC MUCOSA</title><author>MIYAMOTO, Tadahisa ; ITO, Makoto ; MATSUSAKO, Kei ; IKEDA, Kazuo ; JOH, Takasi ; IMAI, Sinpei ; YASUE, Naoji ; YOKOYAMA, Yosihumi ; KATSUMI, Kouhei ; TAKEUCHI, Toshihiko ; NOGUCHI, Yosiki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j1790-559ad7a336534758dae13f81b7f1b742911af61cb2e158a8da8b02f3645a47e93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>jpn</language><creationdate>1986</creationdate><topic>Animals</topic><topic>Calcium - physiology</topic><topic>Gastric Mucosa - secretion</topic><topic>Male</topic><topic>Niacinamide - analogs & derivatives</topic><topic>Niacinamide - pharmacology</topic><topic>Nicorandil</topic><topic>Organ Culture Techniques</topic><topic>Pepsinogens - secretion</topic><topic>Rabbits</topic><topic>Verapamil - pharmacology</topic><toplevel>online_resources</toplevel><creatorcontrib>MIYAMOTO, Tadahisa</creatorcontrib><creatorcontrib>ITO, Makoto</creatorcontrib><creatorcontrib>MATSUSAKO, Kei</creatorcontrib><creatorcontrib>IKEDA, Kazuo</creatorcontrib><creatorcontrib>JOH, Takasi</creatorcontrib><creatorcontrib>IMAI, Sinpei</creatorcontrib><creatorcontrib>YASUE, Naoji</creatorcontrib><creatorcontrib>YOKOYAMA, Yosihumi</creatorcontrib><creatorcontrib>KATSUMI, Kouhei</creatorcontrib><creatorcontrib>TAKEUCHI, Toshihiko</creatorcontrib><creatorcontrib>NOGUCHI, Yosiki</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Nippon Shokakibyo Gakkai Zasshi</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MIYAMOTO, Tadahisa</au><au>ITO, Makoto</au><au>MATSUSAKO, Kei</au><au>IKEDA, Kazuo</au><au>JOH, Takasi</au><au>IMAI, Sinpei</au><au>YASUE, Naoji</au><au>YOKOYAMA, Yosihumi</au><au>KATSUMI, Kouhei</au><au>TAKEUCHI, Toshihiko</au><au>NOGUCHI, Yosiki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>ROLE OF CALCIUM ION ON PEPSINOGEN SECRETION BY ORGAN CULTURE OF RABBIT GASTRIC MUCOSA</atitle><jtitle>Nippon Shokakibyo Gakkai Zasshi</jtitle><addtitle>Nippon Shokakibyo Gakkai Zasshi</addtitle><date>1986-12</date><risdate>1986</risdate><volume>83</volume><issue>12</issue><spage>2514</spage><epage>2519</epage><pages>2514-2519</pages><issn>0446-6586</issn><eissn>1349-7693</eissn><abstract>The role of intracellular and extracellular calcium ion of gastric chief cells for pepsinogen secretion stimulated by carbachol and CCK-8 was evaluated using nicorandil (intracellular calcium antagonist) and verapamil (calcium channel blocker) in organ culture of rabbit gastric mucosa. The pepsinogen secretion stimulated by carbachol (10-4M) was significantly inhibited by 10-5 and 10-4M nicorandil and CCK-8 (10-8M) stimulated pepsinogen secretion was also significantly inhibited by 10-6, 10-5 and 10-4M nicorandil. On the other hand, verapamil (10-6-10-4M) did not inhibit the pepsinogen secretion induced by carbachol and CCK-8. We conclude that a mobilization of the intracellular calcium ion of chief cell, not extracellular one, plays an important role in the mechnisms of pepsinogen secretion stimulated by carbachol and CCK-8.</abstract><cop>Japan</cop><pub>The Japanese Society of Gastroenterology</pub><pmid>2951540</pmid><doi>10.11405/nisshoshi1964.83.2514</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0446-6586 |
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| issn | 0446-6586 1349-7693 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Calcium - physiology Gastric Mucosa - secretion Male Niacinamide - analogs & derivatives Niacinamide - pharmacology Nicorandil Organ Culture Techniques Pepsinogens - secretion Rabbits Verapamil - pharmacology |
| title | ROLE OF CALCIUM ION ON PEPSINOGEN SECRETION BY ORGAN CULTURE OF RABBIT GASTRIC MUCOSA |
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