Isolation of specific proteins affected by estradiol in the arcuate-median eminence of prepuberal female rats
Prepuberal female rats (25 days of age) were injected with estradiol benzoate (EB 10 μg/rat, s.c. in oil) or oil vehicle. Forty-eight hours after treatment, all animals were decapitated, their brains removed and sectioned. The arcuate nucleus of the hypothalamus and median eminence were microdissect...
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| Veröffentlicht in: | Brain research 1986-12, Vol.399 (2), p.379-382 |
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| creator | Rodriguez-Sierra, Jorge F. Heydorn, William E. Joseph Creed, G. Jacobowitz, David M. |
| description | Prepuberal female rats (25 days of age) were injected with estradiol benzoate (EB 10 μg/rat, s.c. in oil) or oil vehicle. Forty-eight hours after treatment, all animals were decapitated, their brains removed and sectioned. The arcuate nucleus of the hypothalamus and median eminence were microdissected and processed for isoelectric focusing followed by slab electrophoresis. The resulting two-dimensional electrophoretic gels were analyzed to quantitate the specific proteins resolved using a scanning microdensitometric method. Out of 235 proteins measured, 8 proteins were found to be significantly increased and 4 were decreased by EB treatment. The proteins which increased in concentration ranged in molecular weight from 15 to 43 kDa and isoelectric points (pI) of 4.9 to 7.0. The 4 proteins decreased by the EB treatment were 44, 67, 74 and 80 kDa in molecular weight and their pI's ranged from 6.5 to 7.1. It is suggested that these proteins might be involved in some of the neuroendocrine effects that are induced by estradiol in this region of the brain. |
| doi_str_mv | 10.1016/0006-8993(86)91532-5 |
| format | Article |
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Forty-eight hours after treatment, all animals were decapitated, their brains removed and sectioned. The arcuate nucleus of the hypothalamus and median eminence were microdissected and processed for isoelectric focusing followed by slab electrophoresis. The resulting two-dimensional electrophoretic gels were analyzed to quantitate the specific proteins resolved using a scanning microdensitometric method. Out of 235 proteins measured, 8 proteins were found to be significantly increased and 4 were decreased by EB treatment. The proteins which increased in concentration ranged in molecular weight from 15 to 43 kDa and isoelectric points (pI) of 4.9 to 7.0. The 4 proteins decreased by the EB treatment were 44, 67, 74 and 80 kDa in molecular weight and their pI's ranged from 6.5 to 7.1. It is suggested that these proteins might be involved in some of the neuroendocrine effects that are induced by estradiol in this region of the brain.</description><identifier>ISSN: 0006-8993</identifier><identifier>EISSN: 1872-6240</identifier><identifier>DOI: 10.1016/0006-8993(86)91532-5</identifier><identifier>PMID: 3828772</identifier><identifier>CODEN: BRREAP</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Animals ; Applied sciences ; Arcuate nucleus ; Arcuate Nucleus of Hypothalamus - drug effects ; Arcuate Nucleus of Hypothalamus - metabolism ; Biological and medical sciences ; Brain protein ; Electrophoresis, Polyacrylamide Gel ; Estradiol ; Exact sciences and technology ; Female ; Fundamental and applied biological sciences. Psychology ; Hormones and neuropeptides. Regulation ; Hypothalamus. Hypophysis. Epiphysis. Urophysis ; Isoelectric Focusing ; Median eminence ; Median Eminence - drug effects ; Median Eminence - metabolism ; Nerve Tissue Proteins - metabolism ; Other techniques and industries ; Rats ; Rats, Inbred Strains ; Sexual Maturation ; Two-dimensional gel electrophoresis ; Vertebrates: endocrinology</subject><ispartof>Brain research, 1986-12, Vol.399 (2), p.379-382</ispartof><rights>1986 Elsevier Science Publishers B.V. 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Forty-eight hours after treatment, all animals were decapitated, their brains removed and sectioned. The arcuate nucleus of the hypothalamus and median eminence were microdissected and processed for isoelectric focusing followed by slab electrophoresis. The resulting two-dimensional electrophoretic gels were analyzed to quantitate the specific proteins resolved using a scanning microdensitometric method. Out of 235 proteins measured, 8 proteins were found to be significantly increased and 4 were decreased by EB treatment. The proteins which increased in concentration ranged in molecular weight from 15 to 43 kDa and isoelectric points (pI) of 4.9 to 7.0. The 4 proteins decreased by the EB treatment were 44, 67, 74 and 80 kDa in molecular weight and their pI's ranged from 6.5 to 7.1. It is suggested that these proteins might be involved in some of the neuroendocrine effects that are induced by estradiol in this region of the brain.</description><subject>Animals</subject><subject>Applied sciences</subject><subject>Arcuate nucleus</subject><subject>Arcuate Nucleus of Hypothalamus - drug effects</subject><subject>Arcuate Nucleus of Hypothalamus - metabolism</subject><subject>Biological and medical sciences</subject><subject>Brain protein</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Estradiol</subject><subject>Exact sciences and technology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hormones and neuropeptides. Regulation</subject><subject>Hypothalamus. Hypophysis. Epiphysis. Urophysis</subject><subject>Isoelectric Focusing</subject><subject>Median eminence</subject><subject>Median Eminence - drug effects</subject><subject>Median Eminence - metabolism</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>Other techniques and industries</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Sexual Maturation</subject><subject>Two-dimensional gel electrophoresis</subject><subject>Vertebrates: endocrinology</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1rFTEUxUNR6mv1P1DIQqRdjM3HTD42BSm2Fgpu7DrcSe7QyHyZzBT635vxPd5SXYVwf_dwzzmEvOfsM2dcXTHGVGWslRdGXVreSFE1J2THjRaVEjV7RXZH5A05y_ln-Upp2Sk5lUYYrcWODPd56mGJ00injuYZfeyip3OaFoxjptB16BcMtH2hmJcEIU49jSNdnpBC8issWA0YIowUhzji6HFTmhPOa4sJetrhAD3SBEt-S1530Gd8d3jPyePt1x8336qH73f3N18eKl_XaqnqRnvQ1qJlrTCgrDVC2NYjU40EG6wUQcggsdW8aT1vmGcKjfXFOxcsyHPyaa9bfPxay91uiNlj38OI05pdsW61YvyfIK8VN7bWBaz3oE9Tzgk7N6c4QHpxnLmtDrdl7basnVHuTx2uKWsfDvprW1I6Lh3yL_OPhzlkD32XYPQxHzEjVaPlf2CieFEFu95jWLJ9jphc9nFrJMRUWnRhin8_9zdoIrLj</recordid><startdate>19861210</startdate><enddate>19861210</enddate><creator>Rodriguez-Sierra, Jorge F.</creator><creator>Heydorn, William E.</creator><creator>Joseph Creed, G.</creator><creator>Jacobowitz, David M.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19861210</creationdate><title>Isolation of specific proteins affected by estradiol in the arcuate-median eminence of prepuberal female rats</title><author>Rodriguez-Sierra, Jorge F. ; Heydorn, William E. ; Joseph Creed, G. ; Jacobowitz, David M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c446t-457ca799e90b28a6998229bce0653a9d932d23d3eb715bc150c06e89c187120d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Animals</topic><topic>Applied sciences</topic><topic>Arcuate nucleus</topic><topic>Arcuate Nucleus of Hypothalamus - drug effects</topic><topic>Arcuate Nucleus of Hypothalamus - metabolism</topic><topic>Biological and medical sciences</topic><topic>Brain protein</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Estradiol</topic><topic>Exact sciences and technology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hormones and neuropeptides. Regulation</topic><topic>Hypothalamus. Hypophysis. Epiphysis. Urophysis</topic><topic>Isoelectric Focusing</topic><topic>Median eminence</topic><topic>Median Eminence - drug effects</topic><topic>Median Eminence - metabolism</topic><topic>Nerve Tissue Proteins - metabolism</topic><topic>Other techniques and industries</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Sexual Maturation</topic><topic>Two-dimensional gel electrophoresis</topic><topic>Vertebrates: endocrinology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rodriguez-Sierra, Jorge F.</creatorcontrib><creatorcontrib>Heydorn, William E.</creatorcontrib><creatorcontrib>Joseph Creed, G.</creatorcontrib><creatorcontrib>Jacobowitz, David M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rodriguez-Sierra, Jorge F.</au><au>Heydorn, William E.</au><au>Joseph Creed, G.</au><au>Jacobowitz, David M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of specific proteins affected by estradiol in the arcuate-median eminence of prepuberal female rats</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>1986-12-10</date><risdate>1986</risdate><volume>399</volume><issue>2</issue><spage>379</spage><epage>382</epage><pages>379-382</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><coden>BRREAP</coden><abstract>Prepuberal female rats (25 days of age) were injected with estradiol benzoate (EB 10 μg/rat, s.c. in oil) or oil vehicle. Forty-eight hours after treatment, all animals were decapitated, their brains removed and sectioned. The arcuate nucleus of the hypothalamus and median eminence were microdissected and processed for isoelectric focusing followed by slab electrophoresis. The resulting two-dimensional electrophoretic gels were analyzed to quantitate the specific proteins resolved using a scanning microdensitometric method. Out of 235 proteins measured, 8 proteins were found to be significantly increased and 4 were decreased by EB treatment. The proteins which increased in concentration ranged in molecular weight from 15 to 43 kDa and isoelectric points (pI) of 4.9 to 7.0. The 4 proteins decreased by the EB treatment were 44, 67, 74 and 80 kDa in molecular weight and their pI's ranged from 6.5 to 7.1. It is suggested that these proteins might be involved in some of the neuroendocrine effects that are induced by estradiol in this region of the brain.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>3828772</pmid><doi>10.1016/0006-8993(86)91532-5</doi><tpages>4</tpages></addata></record> |
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| ispartof | Brain research, 1986-12, Vol.399 (2), p.379-382 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Applied sciences Arcuate nucleus Arcuate Nucleus of Hypothalamus - drug effects Arcuate Nucleus of Hypothalamus - metabolism Biological and medical sciences Brain protein Electrophoresis, Polyacrylamide Gel Estradiol Exact sciences and technology Female Fundamental and applied biological sciences. Psychology Hormones and neuropeptides. Regulation Hypothalamus. Hypophysis. Epiphysis. Urophysis Isoelectric Focusing Median eminence Median Eminence - drug effects Median Eminence - metabolism Nerve Tissue Proteins - metabolism Other techniques and industries Rats Rats, Inbred Strains Sexual Maturation Two-dimensional gel electrophoresis Vertebrates: endocrinology |
| title | Isolation of specific proteins affected by estradiol in the arcuate-median eminence of prepuberal female rats |
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