Molecular Cloning and Differential Expression Patterns of Avian Glutamate Receptor mRNAs

: We have identified and studied potential ionotropic glutamate receptor genes in pigeon brain. Three cDNA clones exhibit significant amino acid sequence identity to members of a rodent ligand‐gated ion channel family. One of them, GluP‐II, encodes a full‐length AMPA‐sensitive glutamate receptor Glu...

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Veröffentlicht in:Journal of neurochemistry 1995-06, Vol.64 (6), p.2413-2426
Hauptverfasser: Ottiger, Hans‐Peter, Gerfin‐Moser, Andrea, Del Principe, Franco, Dutly, Fabrizio, Streit, Peter
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container_end_page 2426
container_issue 6
container_start_page 2413
container_title Journal of neurochemistry
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creator Ottiger, Hans‐Peter
Gerfin‐Moser, Andrea
Del Principe, Franco
Dutly, Fabrizio
Streit, Peter
description : We have identified and studied potential ionotropic glutamate receptor genes in pigeon brain. Three cDNA clones exhibit significant amino acid sequence identity to members of a rodent ligand‐gated ion channel family. One of them, GluP‐II, encodes a full‐length AMPA‐sensitive glutamate receptor GluR2 (GluR‐B) homologue, whereas the other two partial clones, designated as GluP‐III and ‐IV, are nearly identical to rodent GluR3 (GluR‐C) and GluR4 (GluR‐D) receptor subunits. Northern analysis demonstrated that the avian genes are widely expressed in the brain. Within the brain regions analyzed by in situ hybridization histochemistry, the three avian GluR subunits showed distinct and regionally specific mRNA expression patterns in the adult. Most of the differences in their expression were observed in cell types of the telencephalon, certain thalamic nuclei, the optic tectum, and the cerebellar cortex. A particularly striking finding was the expression of GluP‐II in Golgi epithelial/Bergmann glial cells. In contrast, Bergmann glial cells in rat cerebellum do not express GluR2 (GluR‐B) subunit genes. Immunoreactivity for a monoclonal sequence‐specific antipeptide antibody was widespread and most prominent in Purkinje cell perikarya and their dendrites, neuronal cell bodies of the ectostriatum, and the deep optic tectum. These results demonstrate the existence of multiple subunits of the ionotropic glutamate receptor channel family in avians. Excitatory amino acid receptor genes appear to be highly conserved during evolution.
doi_str_mv 10.1046/j.1471-4159.1995.64062413.x
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Three cDNA clones exhibit significant amino acid sequence identity to members of a rodent ligand‐gated ion channel family. One of them, GluP‐II, encodes a full‐length AMPA‐sensitive glutamate receptor GluR2 (GluR‐B) homologue, whereas the other two partial clones, designated as GluP‐III and ‐IV, are nearly identical to rodent GluR3 (GluR‐C) and GluR4 (GluR‐D) receptor subunits. Northern analysis demonstrated that the avian genes are widely expressed in the brain. Within the brain regions analyzed by in situ hybridization histochemistry, the three avian GluR subunits showed distinct and regionally specific mRNA expression patterns in the adult. Most of the differences in their expression were observed in cell types of the telencephalon, certain thalamic nuclei, the optic tectum, and the cerebellar cortex. A particularly striking finding was the expression of GluP‐II in Golgi epithelial/Bergmann glial cells. In contrast, Bergmann glial cells in rat cerebellum do not express GluR2 (GluR‐B) subunit genes. Immunoreactivity for a monoclonal sequence‐specific antipeptide antibody was widespread and most prominent in Purkinje cell perikarya and their dendrites, neuronal cell bodies of the ectostriatum, and the deep optic tectum. These results demonstrate the existence of multiple subunits of the ionotropic glutamate receptor channel family in avians. 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Psychology ; Immunocytochemistry ; Immunohistochemistry ; In Situ Hybridization ; Molecular and cellular biology ; Molecular Sequence Data ; Pigeon glutamate receptor subunit ; Receptors, Glutamate - classification ; Receptors, Glutamate - genetics ; Receptors, Glutamate - metabolism ; RNA, Messenger - metabolism</subject><ispartof>Journal of neurochemistry, 1995-06, Vol.64 (6), p.2413-2426</ispartof><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4183-942cbcd7b30b7e2b29f4441641236e4eb11bf77ccdbdb77997a0f746fe2a0bc43</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1471-4159.1995.64062413.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1471-4159.1995.64062413.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=3519493$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7760022$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ottiger, Hans‐Peter</creatorcontrib><creatorcontrib>Gerfin‐Moser, Andrea</creatorcontrib><creatorcontrib>Del Principe, Franco</creatorcontrib><creatorcontrib>Dutly, Fabrizio</creatorcontrib><creatorcontrib>Streit, Peter</creatorcontrib><title>Molecular Cloning and Differential Expression Patterns of Avian Glutamate Receptor mRNAs</title><title>Journal of neurochemistry</title><addtitle>J Neurochem</addtitle><description>: We have identified and studied potential ionotropic glutamate receptor genes in pigeon brain. Three cDNA clones exhibit significant amino acid sequence identity to members of a rodent ligand‐gated ion channel family. One of them, GluP‐II, encodes a full‐length AMPA‐sensitive glutamate receptor GluR2 (GluR‐B) homologue, whereas the other two partial clones, designated as GluP‐III and ‐IV, are nearly identical to rodent GluR3 (GluR‐C) and GluR4 (GluR‐D) receptor subunits. Northern analysis demonstrated that the avian genes are widely expressed in the brain. Within the brain regions analyzed by in situ hybridization histochemistry, the three avian GluR subunits showed distinct and regionally specific mRNA expression patterns in the adult. Most of the differences in their expression were observed in cell types of the telencephalon, certain thalamic nuclei, the optic tectum, and the cerebellar cortex. A particularly striking finding was the expression of GluP‐II in Golgi epithelial/Bergmann glial cells. In contrast, Bergmann glial cells in rat cerebellum do not express GluR2 (GluR‐B) subunit genes. Immunoreactivity for a monoclonal sequence‐specific antipeptide antibody was widespread and most prominent in Purkinje cell perikarya and their dendrites, neuronal cell bodies of the ectostriatum, and the deep optic tectum. These results demonstrate the existence of multiple subunits of the ionotropic glutamate receptor channel family in avians. Excitatory amino acid receptor genes appear to be highly conserved during evolution.</description><subject>Amino Acid Sequence</subject><subject>Aminoacid receptors (glycine, glutamate, gaba)</subject><subject>Animals</subject><subject>Aves</subject><subject>Avian cerebellum</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Blotting, Western</subject><subject>Brain - metabolism</subject><subject>cDNA cloning</subject><subject>Cell receptors</subject><subject>Cell structures and functions</subject><subject>Cloning, Molecular</subject><subject>Columbidae - genetics</subject><subject>DNA, Complementary - genetics</subject><subject>DNA, Complementary - isolation &amp; purification</subject><subject>Excitatory amino acid receptor</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immunocytochemistry</subject><subject>Immunohistochemistry</subject><subject>In Situ Hybridization</subject><subject>Molecular and cellular biology</subject><subject>Molecular Sequence Data</subject><subject>Pigeon glutamate receptor subunit</subject><subject>Receptors, Glutamate - classification</subject><subject>Receptors, Glutamate - genetics</subject><subject>Receptors, Glutamate - metabolism</subject><subject>RNA, Messenger - metabolism</subject><issn>0022-3042</issn><issn>1471-4159</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkUuLFDEUhYMoY8_oTxACirsq8-qkg6um56EyjjIouAtJ6kbSpKrapEp7_r1VdE9vxdXlcr774ByEXlNSUyLku21NhaKVoEtdU62XtRREMkF5vX-CFiftKVoQwljFiWDP0XkpW0KoFJKeoTOl5Kwt0I_PfQI_JpvxJvVd7H5i2zX4MoYAGboh2oSv9rsMpcS-w1_tMEDuCu4DXv-OtsM3aRxsawfA9-BhN_QZt_d36_ICPQs2FXh5rBfo-_XVt82H6vbLzcfN-rbygq54pQXzzjfKceIUMMd0EEJMb1LGJQhwlLqglPeNa5xSWitLghIyALPEecEv0NvD3l3uf41QBtPG4iEl20E_FqMU05JO2_4FUrmiguvVBL4_gD73pWQIZpdja_ODocTMAZitmU02s8lmDsA8BmD20_Sr45nRtdCcZo-OT_qbo26Ltylk2_lYThhfUi00n7DLA_YnJnj4nw_Mp7vNY8f_AlBjoqM</recordid><startdate>199506</startdate><enddate>199506</enddate><creator>Ottiger, Hans‐Peter</creator><creator>Gerfin‐Moser, Andrea</creator><creator>Del Principe, Franco</creator><creator>Dutly, Fabrizio</creator><creator>Streit, Peter</creator><general>Blackwell Science Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>199506</creationdate><title>Molecular Cloning and Differential Expression Patterns of Avian Glutamate Receptor mRNAs</title><author>Ottiger, Hans‐Peter ; Gerfin‐Moser, Andrea ; Del Principe, Franco ; Dutly, Fabrizio ; Streit, Peter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4183-942cbcd7b30b7e2b29f4441641236e4eb11bf77ccdbdb77997a0f746fe2a0bc43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Amino Acid Sequence</topic><topic>Aminoacid receptors (glycine, glutamate, gaba)</topic><topic>Animals</topic><topic>Aves</topic><topic>Avian cerebellum</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>Blotting, Western</topic><topic>Brain - metabolism</topic><topic>cDNA cloning</topic><topic>Cell receptors</topic><topic>Cell structures and functions</topic><topic>Cloning, Molecular</topic><topic>Columbidae - genetics</topic><topic>DNA, Complementary - genetics</topic><topic>DNA, Complementary - isolation &amp; purification</topic><topic>Excitatory amino acid receptor</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Immunocytochemistry</topic><topic>Immunohistochemistry</topic><topic>In Situ Hybridization</topic><topic>Molecular and cellular biology</topic><topic>Molecular Sequence Data</topic><topic>Pigeon glutamate receptor subunit</topic><topic>Receptors, Glutamate - classification</topic><topic>Receptors, Glutamate - genetics</topic><topic>Receptors, Glutamate - metabolism</topic><topic>RNA, Messenger - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ottiger, Hans‐Peter</creatorcontrib><creatorcontrib>Gerfin‐Moser, Andrea</creatorcontrib><creatorcontrib>Del Principe, Franco</creatorcontrib><creatorcontrib>Dutly, Fabrizio</creatorcontrib><creatorcontrib>Streit, Peter</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neurochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ottiger, Hans‐Peter</au><au>Gerfin‐Moser, Andrea</au><au>Del Principe, Franco</au><au>Dutly, Fabrizio</au><au>Streit, Peter</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular Cloning and Differential Expression Patterns of Avian Glutamate Receptor mRNAs</atitle><jtitle>Journal of neurochemistry</jtitle><addtitle>J Neurochem</addtitle><date>1995-06</date><risdate>1995</risdate><volume>64</volume><issue>6</issue><spage>2413</spage><epage>2426</epage><pages>2413-2426</pages><issn>0022-3042</issn><eissn>1471-4159</eissn><coden>JONRA9</coden><abstract>: We have identified and studied potential ionotropic glutamate receptor genes in pigeon brain. Three cDNA clones exhibit significant amino acid sequence identity to members of a rodent ligand‐gated ion channel family. One of them, GluP‐II, encodes a full‐length AMPA‐sensitive glutamate receptor GluR2 (GluR‐B) homologue, whereas the other two partial clones, designated as GluP‐III and ‐IV, are nearly identical to rodent GluR3 (GluR‐C) and GluR4 (GluR‐D) receptor subunits. Northern analysis demonstrated that the avian genes are widely expressed in the brain. Within the brain regions analyzed by in situ hybridization histochemistry, the three avian GluR subunits showed distinct and regionally specific mRNA expression patterns in the adult. Most of the differences in their expression were observed in cell types of the telencephalon, certain thalamic nuclei, the optic tectum, and the cerebellar cortex. A particularly striking finding was the expression of GluP‐II in Golgi epithelial/Bergmann glial cells. In contrast, Bergmann glial cells in rat cerebellum do not express GluR2 (GluR‐B) subunit genes. Immunoreactivity for a monoclonal sequence‐specific antipeptide antibody was widespread and most prominent in Purkinje cell perikarya and their dendrites, neuronal cell bodies of the ectostriatum, and the deep optic tectum. These results demonstrate the existence of multiple subunits of the ionotropic glutamate receptor channel family in avians. Excitatory amino acid receptor genes appear to be highly conserved during evolution.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>7760022</pmid><doi>10.1046/j.1471-4159.1995.64062413.x</doi><tpages>14</tpages></addata></record>
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1471-4159
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subjects Amino Acid Sequence
Aminoacid receptors (glycine, glutamate, gaba)
Animals
Aves
Avian cerebellum
Base Sequence
Biological and medical sciences
Blotting, Northern
Blotting, Western
Brain - metabolism
cDNA cloning
Cell receptors
Cell structures and functions
Cloning, Molecular
Columbidae - genetics
DNA, Complementary - genetics
DNA, Complementary - isolation & purification
Excitatory amino acid receptor
Fundamental and applied biological sciences. Psychology
Immunocytochemistry
Immunohistochemistry
In Situ Hybridization
Molecular and cellular biology
Molecular Sequence Data
Pigeon glutamate receptor subunit
Receptors, Glutamate - classification
Receptors, Glutamate - genetics
Receptors, Glutamate - metabolism
RNA, Messenger - metabolism
title Molecular Cloning and Differential Expression Patterns of Avian Glutamate Receptor mRNAs
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