Nucleic Acid Affinity Chromatography: Preparation and Characterization of Double-Stranded RNA Agarose

An improved method for the preparation of a double-stranded RNA-coupled resin is described. Periodate-oxidized double-stranded RNA, in the form of either purified reovirus double-stranded RNA or poly(rl)·poly(rC), was covalently attached to agarose-adipic acid hydrazide. The resulting affinity resin...

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Veröffentlicht in:	Protein expression and purification 1995-02, Vol.6 (1), p.25-32
Hauptverfasser:	Langland, J.O., Pettiford, S.M., Jacobs, B.L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Binding, Competitive Capsid Proteins Cells, Cultured Chromatography, Affinity - methods Electrophoresis, Polyacrylamide Gel Interferons - pharmacology Protein Kinases - isolation & purification Reoviridae - genetics RNA, Double-Stranded - chemistry RNA, Double-Stranded - genetics RNA, Double-Stranded - metabolism RNA-Binding Proteins - isolation & purification RNA-Binding Proteins - metabolism Sepharose - chemistry Viral Proteins - isolation & purification Viral Proteins - metabolism
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