Effects of oligosaccharide binding on glycogen debranching enzyme activity and conformation

Glycogen debranching enzyme contains two catalytic activities (4-alpha-glucanotransferase and amylo-1,6-glucosidase) on its single polypeptide chain, and they are affected differently by the binding of oligosaccharides. Glucose, maltose, and maltotriose are competitive inhibitors of the amylo-1,6-gl...

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Veröffentlicht in:	Biochemistry (Easton) 1995-05, Vol.34 (21), p.7056-7061
Hauptverfasser:	Liu, Wei, Madsen, Neil B, Fan, Baochen, Zucker, Karl A, Glew, Robert H, Fry, Donald E
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Circular Dichroism Glucose - metabolism Glucosidases - metabolism Glycogen - metabolism Glycogen Debranching Enzyme System - chemistry Glycogen Debranching Enzyme System - metabolism Kinetics Muscles - enzymology Oligosaccharides - metabolism Protein Conformation Rabbits Spectrometry, Fluorescence Substrate Specificity
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