Genetic instability assessed by sister chromatid exchange analysis in the dunning R-3327 rat prostatic adenocarcinoma model and its relationship to metastatic potential

The original Dunning R‐3327 tumor, described in 1961, has given rise to distinct sublines of different metastatic potentials. The different phenotypes cannot be explained by differences in chromosomal number, DNA content, or nuclear pleomorphism. Sister chromatid exchange is an interchange between t...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:The Prostate 1995-05, Vol.26 (5), p.247-252
Hauptverfasser: Sharief, Yousuf, Mohler, James L.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 252
container_issue 5
container_start_page 247
container_title The Prostate
container_volume 26
creator Sharief, Yousuf
Mohler, James L.
description The original Dunning R‐3327 tumor, described in 1961, has given rise to distinct sublines of different metastatic potentials. The different phenotypes cannot be explained by differences in chromosomal number, DNA content, or nuclear pleomorphism. Sister chromatid exchange is an interchange between two strands of DNA indicative of DNA damage. The frequency of sister chromatid exchanges is a well‐accepted measure of genetic instability. To determine whether an assay of genetic instability could distinguish sublines capable of generating cells of the metastatic phenotype, cells from three sublines of low (90%) metastatic potential were cultured in 10 μM 5‐bromodeoxyuridine to label DNA. Chromosome preparations were made and sister chromatids were differentiated with Hoechst 33258 dye and Giemsa stain. Sixty metaphase spreads from each subline were scored for SCE and chromosome number. The low metastatic sublines G, AT‐1, and AT‐2 had 0.32 ± standard deviation 0.10, 0.38 ± 0.12, and 0.14 ± 0.05 sister chromatid exchanges per chromosome, respectively. The high metastatic sublines AT‐3, MAT‐Lu, and MAT‐LyLu had 0.55 ± 0.17, 0.32 ± 0.1, and 0.33 ± 0.2 sister chromatid exchanges per chromosome, respectively. Subline differences in metastatic potentials cannot be explained by incidences of sister chromatid exchanges.
doi_str_mv 10.1002/pros.2990260504
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_77282706</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>77282706</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3364-5f2c9df619c66ca2b7c50f42c3ef9e50a21a94770da1af3f422848ac2aa03e0d3</originalsourceid><addsrcrecordid>eNqFkUFv1DAQhS0EKkvhzAnJJ25pJ3YSb8QJLXRbqaKogDhas_aka0icre0VzT_iZ-JoV0WckCz58N77NDOPsdclnJUA4nwXxngm2hZEAzVUT9iihFYVAFX9lC1AKCiqUqrn7EWMPwByBsQJO1GqlgraBfu9Jk_JGe58TLhxvUsTxxgpP8s3E48uJgrcbMM4YHKW04PZor8jjh77Kcs5ytOWuN177_wdvy2kFIoHTHyeLuGMR0t-NBiM85nDh9FSnwmWuxR5oD6bRh-3bsfTyAdKeMztxkQ-Oexfsmcd9pFeHf9T9u3i49fVZXF9s75avb8ujJRNVdSdMK3tmrI1TWNQbJSpoauEkdS1VAOKEttKKbBYYiezIpbVEo1ABElg5Sl7e-Dm2e_3FJMeXDTU9-hp3EetlFjmqzbZeH4wmrxkDNTpXXADhkmXoOdu9Ly9_ttNTrw5ovebgeyj_1hG1t8d9F-up-l_OP359ubLP_TikJ4Le3hMY_ipGyVVrb9_WuuL1Wqp1pn4Qf4Bg9Svpw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>77282706</pqid></control><display><type>article</type><title>Genetic instability assessed by sister chromatid exchange analysis in the dunning R-3327 rat prostatic adenocarcinoma model and its relationship to metastatic potential</title><source>MEDLINE</source><source>Wiley Blackwell Journals</source><creator>Sharief, Yousuf ; Mohler, James L.</creator><creatorcontrib>Sharief, Yousuf ; Mohler, James L.</creatorcontrib><description>The original Dunning R‐3327 tumor, described in 1961, has given rise to distinct sublines of different metastatic potentials. The different phenotypes cannot be explained by differences in chromosomal number, DNA content, or nuclear pleomorphism. Sister chromatid exchange is an interchange between two strands of DNA indicative of DNA damage. The frequency of sister chromatid exchanges is a well‐accepted measure of genetic instability. To determine whether an assay of genetic instability could distinguish sublines capable of generating cells of the metastatic phenotype, cells from three sublines of low (&lt;10%) metastatic potential and three sublines of high (&gt;90%) metastatic potential were cultured in 10 μM 5‐bromodeoxyuridine to label DNA. Chromosome preparations were made and sister chromatids were differentiated with Hoechst 33258 dye and Giemsa stain. Sixty metaphase spreads from each subline were scored for SCE and chromosome number. The low metastatic sublines G, AT‐1, and AT‐2 had 0.32 ± standard deviation 0.10, 0.38 ± 0.12, and 0.14 ± 0.05 sister chromatid exchanges per chromosome, respectively. The high metastatic sublines AT‐3, MAT‐Lu, and MAT‐LyLu had 0.55 ± 0.17, 0.32 ± 0.1, and 0.33 ± 0.2 sister chromatid exchanges per chromosome, respectively. Subline differences in metastatic potentials cannot be explained by incidences of sister chromatid exchanges.</description><identifier>ISSN: 0270-4137</identifier><identifier>EISSN: 1097-0045</identifier><identifier>DOI: 10.1002/pros.2990260504</identifier><identifier>PMID: 7753709</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Adenocarcinoma - genetics ; Adenocarcinoma - secondary ; Animals ; Chromosomes ; genetic instability ; Male ; prostatic carcinoma ; Prostatic Neoplasms - genetics ; Prostatic Neoplasms - pathology ; Rats ; Sister Chromatid Exchange ; Tumor Cells, Cultured</subject><ispartof>The Prostate, 1995-05, Vol.26 (5), p.247-252</ispartof><rights>Copyright © 1995 Wiley‐Liss, Inc., A Wiley Company</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3364-5f2c9df619c66ca2b7c50f42c3ef9e50a21a94770da1af3f422848ac2aa03e0d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fpros.2990260504$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fpros.2990260504$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7753709$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sharief, Yousuf</creatorcontrib><creatorcontrib>Mohler, James L.</creatorcontrib><title>Genetic instability assessed by sister chromatid exchange analysis in the dunning R-3327 rat prostatic adenocarcinoma model and its relationship to metastatic potential</title><title>The Prostate</title><addtitle>Prostate</addtitle><description>The original Dunning R‐3327 tumor, described in 1961, has given rise to distinct sublines of different metastatic potentials. The different phenotypes cannot be explained by differences in chromosomal number, DNA content, or nuclear pleomorphism. Sister chromatid exchange is an interchange between two strands of DNA indicative of DNA damage. The frequency of sister chromatid exchanges is a well‐accepted measure of genetic instability. To determine whether an assay of genetic instability could distinguish sublines capable of generating cells of the metastatic phenotype, cells from three sublines of low (&lt;10%) metastatic potential and three sublines of high (&gt;90%) metastatic potential were cultured in 10 μM 5‐bromodeoxyuridine to label DNA. Chromosome preparations were made and sister chromatids were differentiated with Hoechst 33258 dye and Giemsa stain. Sixty metaphase spreads from each subline were scored for SCE and chromosome number. The low metastatic sublines G, AT‐1, and AT‐2 had 0.32 ± standard deviation 0.10, 0.38 ± 0.12, and 0.14 ± 0.05 sister chromatid exchanges per chromosome, respectively. The high metastatic sublines AT‐3, MAT‐Lu, and MAT‐LyLu had 0.55 ± 0.17, 0.32 ± 0.1, and 0.33 ± 0.2 sister chromatid exchanges per chromosome, respectively. Subline differences in metastatic potentials cannot be explained by incidences of sister chromatid exchanges.</description><subject>Adenocarcinoma - genetics</subject><subject>Adenocarcinoma - secondary</subject><subject>Animals</subject><subject>Chromosomes</subject><subject>genetic instability</subject><subject>Male</subject><subject>prostatic carcinoma</subject><subject>Prostatic Neoplasms - genetics</subject><subject>Prostatic Neoplasms - pathology</subject><subject>Rats</subject><subject>Sister Chromatid Exchange</subject><subject>Tumor Cells, Cultured</subject><issn>0270-4137</issn><issn>1097-0045</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFv1DAQhS0EKkvhzAnJJ25pJ3YSb8QJLXRbqaKogDhas_aka0icre0VzT_iZ-JoV0WckCz58N77NDOPsdclnJUA4nwXxngm2hZEAzVUT9iihFYVAFX9lC1AKCiqUqrn7EWMPwByBsQJO1GqlgraBfu9Jk_JGe58TLhxvUsTxxgpP8s3E48uJgrcbMM4YHKW04PZor8jjh77Kcs5ytOWuN177_wdvy2kFIoHTHyeLuGMR0t-NBiM85nDh9FSnwmWuxR5oD6bRh-3bsfTyAdKeMztxkQ-Oexfsmcd9pFeHf9T9u3i49fVZXF9s75avb8ujJRNVdSdMK3tmrI1TWNQbJSpoauEkdS1VAOKEttKKbBYYiezIpbVEo1ABElg5Sl7e-Dm2e_3FJMeXDTU9-hp3EetlFjmqzbZeH4wmrxkDNTpXXADhkmXoOdu9Ly9_ttNTrw5ovebgeyj_1hG1t8d9F-up-l_OP359ubLP_TikJ4Le3hMY_ipGyVVrb9_WuuL1Wqp1pn4Qf4Bg9Svpw</recordid><startdate>199505</startdate><enddate>199505</enddate><creator>Sharief, Yousuf</creator><creator>Mohler, James L.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199505</creationdate><title>Genetic instability assessed by sister chromatid exchange analysis in the dunning R-3327 rat prostatic adenocarcinoma model and its relationship to metastatic potential</title><author>Sharief, Yousuf ; Mohler, James L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3364-5f2c9df619c66ca2b7c50f42c3ef9e50a21a94770da1af3f422848ac2aa03e0d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Adenocarcinoma - genetics</topic><topic>Adenocarcinoma - secondary</topic><topic>Animals</topic><topic>Chromosomes</topic><topic>genetic instability</topic><topic>Male</topic><topic>prostatic carcinoma</topic><topic>Prostatic Neoplasms - genetics</topic><topic>Prostatic Neoplasms - pathology</topic><topic>Rats</topic><topic>Sister Chromatid Exchange</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sharief, Yousuf</creatorcontrib><creatorcontrib>Mohler, James L.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Prostate</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sharief, Yousuf</au><au>Mohler, James L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic instability assessed by sister chromatid exchange analysis in the dunning R-3327 rat prostatic adenocarcinoma model and its relationship to metastatic potential</atitle><jtitle>The Prostate</jtitle><addtitle>Prostate</addtitle><date>1995-05</date><risdate>1995</risdate><volume>26</volume><issue>5</issue><spage>247</spage><epage>252</epage><pages>247-252</pages><issn>0270-4137</issn><eissn>1097-0045</eissn><abstract>The original Dunning R‐3327 tumor, described in 1961, has given rise to distinct sublines of different metastatic potentials. The different phenotypes cannot be explained by differences in chromosomal number, DNA content, or nuclear pleomorphism. Sister chromatid exchange is an interchange between two strands of DNA indicative of DNA damage. The frequency of sister chromatid exchanges is a well‐accepted measure of genetic instability. To determine whether an assay of genetic instability could distinguish sublines capable of generating cells of the metastatic phenotype, cells from three sublines of low (&lt;10%) metastatic potential and three sublines of high (&gt;90%) metastatic potential were cultured in 10 μM 5‐bromodeoxyuridine to label DNA. Chromosome preparations were made and sister chromatids were differentiated with Hoechst 33258 dye and Giemsa stain. Sixty metaphase spreads from each subline were scored for SCE and chromosome number. The low metastatic sublines G, AT‐1, and AT‐2 had 0.32 ± standard deviation 0.10, 0.38 ± 0.12, and 0.14 ± 0.05 sister chromatid exchanges per chromosome, respectively. The high metastatic sublines AT‐3, MAT‐Lu, and MAT‐LyLu had 0.55 ± 0.17, 0.32 ± 0.1, and 0.33 ± 0.2 sister chromatid exchanges per chromosome, respectively. Subline differences in metastatic potentials cannot be explained by incidences of sister chromatid exchanges.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>7753709</pmid><doi>10.1002/pros.2990260504</doi><tpages>6</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0270-4137
ispartof The Prostate, 1995-05, Vol.26 (5), p.247-252
issn 0270-4137
1097-0045
language eng
recordid cdi_proquest_miscellaneous_77282706
source MEDLINE; Wiley Blackwell Journals
subjects Adenocarcinoma - genetics
Adenocarcinoma - secondary
Animals
Chromosomes
genetic instability
Male
prostatic carcinoma
Prostatic Neoplasms - genetics
Prostatic Neoplasms - pathology
Rats
Sister Chromatid Exchange
Tumor Cells, Cultured
title Genetic instability assessed by sister chromatid exchange analysis in the dunning R-3327 rat prostatic adenocarcinoma model and its relationship to metastatic potential
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T05%3A36%3A34IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Genetic%20instability%20assessed%20by%20sister%20chromatid%20exchange%20analysis%20in%20the%20dunning%20R-3327%20rat%20prostatic%20adenocarcinoma%20model%20and%20its%20relationship%20to%20metastatic%20potential&rft.jtitle=The%20Prostate&rft.au=Sharief,%20Yousuf&rft.date=1995-05&rft.volume=26&rft.issue=5&rft.spage=247&rft.epage=252&rft.pages=247-252&rft.issn=0270-4137&rft.eissn=1097-0045&rft_id=info:doi/10.1002/pros.2990260504&rft_dat=%3Cproquest_cross%3E77282706%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=77282706&rft_id=info:pmid/7753709&rfr_iscdi=true