Absence of xanthine oxidase or xanthine dehydrogenase in the rabbit myocardium
We directly measured the activity of the enzymes xanthine oxidase and xanthine dehydrogenase in rabbit and rat hearts, using a sensitive radio-chemical assay. Neither xanthine oxidase activity nor xanthine dehydrogenase activity was detected in the rabbit heart. In the rat heart, xanthine oxidase ac...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1986-12, Vol.141 (3), p.1104-1108 |
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| container_end_page | 1108 |
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| container_issue | 3 |
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| container_title | Biochemical and biophysical research communications |
| container_volume | 141 |
| creator | Grum, Cyril M. Ragsdale, Richard A. Ketai, Loren H. Shlafer, Marshal |
| description | We directly measured the activity of the enzymes xanthine oxidase and xanthine dehydrogenase in rabbit and rat hearts, using a sensitive radio-chemical assay. Neither xanthine oxidase activity nor xanthine dehydrogenase activity was detected in the rabbit heart. In the rat heart, xanthine oxidase activity was 9.1 ± 0.5 mIU per gram wet weight and xanthine dehydrogenase activity was 53.0 ± 1.9 mIU per gram wet weight. These results argue against the involvement of the xanthine oxidase/xanthine dehydrogenase system as a mechanism of tissue injury in the rabbit heart, and suggest that the ability of allopurinol to protect the rabbit heart against hypoxic or ischemic damage must be due to a mechanism other than inhibition of these enzymes. |
| doi_str_mv | 10.1016/S0006-291X(86)80157-7 |
| format | Article |
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Neither xanthine oxidase activity nor xanthine dehydrogenase activity was detected in the rabbit heart. In the rat heart, xanthine oxidase activity was 9.1 ± 0.5 mIU per gram wet weight and xanthine dehydrogenase activity was 53.0 ± 1.9 mIU per gram wet weight. 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These results argue against the involvement of the xanthine oxidase/xanthine dehydrogenase system as a mechanism of tissue injury in the rabbit heart, and suggest that the ability of allopurinol to protect the rabbit heart against hypoxic or ischemic damage must be due to a mechanism other than inhibition of these enzymes.</description><subject>Animals</subject><subject>Applied sciences</subject><subject>Exact sciences and technology</subject><subject>Ketone Oxidoreductases - metabolism</subject><subject>Myocardium - enzymology</subject><subject>Other techniques and industries</subject><subject>Rabbits - metabolism</subject><subject>Rats</subject><subject>Species Specificity</subject><subject>Xanthine Dehydrogenase - metabolism</subject><subject>Xanthine Oxidase - metabolism</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1r3DAQhkVpSDcfPyHgQynNwenIljXyqYSQLwjJIQnkJsbSuKuytlPJW7L_vt7ssj0GHUbM-4w0PEKcSDiTIPWPRwDQeVHLl-9GnxqQFeb4Scwk1JAXEtRnMdshX8RBSr8BpFS63hf7pdKmVmYm7s-bxL3jbGizN-rHeein-1vwlKYa__c8z1c-Dr-4Xyehz8Y5Z5GaJoxZtxocRR-W3ZHYa2mR-HhbD8Xz1eXTxU1-93B9e3F-lztlzJgXLZeIBpHZeENOgibTesBWKd8oono6VKD2EsBB3WpGx1hWilQDDZWH4tvm3dc4_FlyGm0XkuPFgnoelskiFlhhISew2oAuDilFbu1rDB3FlZVg1x7tu0e7lmSNtu8eLU5zJ9sPlk3Hfje1FTflX7c5JUeLNlLvQtphWBUGq3LCfm4wnmT8DRxtcmHt24fIbrR-CB8s8g8yTpBs</recordid><startdate>19861230</startdate><enddate>19861230</enddate><creator>Grum, Cyril M.</creator><creator>Ragsdale, Richard A.</creator><creator>Ketai, Loren H.</creator><creator>Shlafer, Marshal</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19861230</creationdate><title>Absence of xanthine oxidase or xanthine dehydrogenase in the rabbit myocardium</title><author>Grum, Cyril M. ; Ragsdale, Richard A. ; Ketai, Loren H. ; Shlafer, Marshal</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c488t-2fe377877ee8d8ac106a8fd07f44db4aa9a9aa276d100c09f6e7ce7354a4b0ba3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Animals</topic><topic>Applied sciences</topic><topic>Exact sciences and technology</topic><topic>Ketone Oxidoreductases - metabolism</topic><topic>Myocardium - enzymology</topic><topic>Other techniques and industries</topic><topic>Rabbits - metabolism</topic><topic>Rats</topic><topic>Species Specificity</topic><topic>Xanthine Dehydrogenase - metabolism</topic><topic>Xanthine Oxidase - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Grum, Cyril M.</creatorcontrib><creatorcontrib>Ragsdale, Richard A.</creatorcontrib><creatorcontrib>Ketai, Loren H.</creatorcontrib><creatorcontrib>Shlafer, Marshal</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Grum, Cyril M.</au><au>Ragsdale, Richard A.</au><au>Ketai, Loren H.</au><au>Shlafer, Marshal</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Absence of xanthine oxidase or xanthine dehydrogenase in the rabbit myocardium</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1986-12-30</date><risdate>1986</risdate><volume>141</volume><issue>3</issue><spage>1104</spage><epage>1108</epage><pages>1104-1108</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>We directly measured the activity of the enzymes xanthine oxidase and xanthine dehydrogenase in rabbit and rat hearts, using a sensitive radio-chemical assay. 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| ispartof | Biochemical and biophysical research communications, 1986-12, Vol.141 (3), p.1104-1108 |
| issn | 0006-291X 1090-2104 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77275721 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Applied sciences Exact sciences and technology Ketone Oxidoreductases - metabolism Myocardium - enzymology Other techniques and industries Rabbits - metabolism Rats Species Specificity Xanthine Dehydrogenase - metabolism Xanthine Oxidase - metabolism |
| title | Absence of xanthine oxidase or xanthine dehydrogenase in the rabbit myocardium |
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