Regulation of the nah and sal operons of plasmid NAH7: Evidence for a new function in nahR

Regulation of the nah and sal operons of plasmid NAH7: Evidence for a new function in nahR

The catabolism of naphthalene and salicylate is specified by two operons on an 80 Kb metabolic plasmid, NAH7. These operons, nah and sal are carried on the contiguous 30 Kb EcoRI-A, C fragments, and are under positive control of a regulator region, nahR. Five Nah − Sal − Tn5 insertion mutants form t...

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Veröffentlicht in:Biochemical and biophysical research communications 1986-12, Vol.141 (3), p.986-992
Hauptverfasser: You, I.-S., Gunsalus, I.C.
Format: Artikel
Sprache:eng
Schlagworte:
Cloning, Molecular
Deoxyribonuclease HindIII
DNA Restriction Enzymes
DNA, Recombinant
Escherichia coli - genetics
gene mapping
gene regulation
Genes, Regulator
naphthalene
Naphthalenes - metabolism
Nucleic Acid Hybridization
Operon
operons
Plasmids
Pseudomonas - genetics
Pseudomonas putida
Salicylates - metabolism
Salicylic Acid
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Zusammenfassung:The catabolism of naphthalene and salicylate is specified by two operons on an 80 Kb metabolic plasmid, NAH7. These operons, nah and sal are carried on the contiguous 30 Kb EcoRI-A, C fragments, and are under positive control of a regulator region, nahR. Five Nah − Sal − Tn5 insertion mutants form two complementation groups: A= nahR203, nahR204; and B= nahR201, nahR202, nahR205. The physical and genetic maps assign the nahR location to the 15.7–17.2 Kb region of the EcoRI-A fragment, with suggestion of more than one control gene.
ISSN:0006-291X
1090-2104
DOI:10.1016/S0006-291X(86)80141-3