Identification of early genes in the Drosophila immune response by PCR-based differential display: the Attacin A gene and the evolution of attacin-like proteins
We are using the PCR-based differential display technique to isolate genes which are induced during the immune response in Drosophila. In this way, a cDNA clone for a member of the attacin family of antibacterial proteins was isolated. The corresponding Attacin A (AttA) gene is localized at 51A-B on...
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| Veröffentlicht in: | Insect biochemistry and molecular biology 1995-04, Vol.25 (4), p.511-518 |
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| creator | Åsling, Bengt Dushay, Mitchell S. Hultmark, Dan |
| description | We are using the PCR-based differential display technique to isolate genes which are induced during the immune response in
Drosophila. In this way, a cDNA clone for a member of the attacin family of antibacterial proteins was isolated. The corresponding
Attacin A (AttA) gene is localized at 51A-B on the second chromosome, and it is closely linked to at least one more cross-hybridizing gene. Injection of bacteria induces a 0.8 kb transcript, with expression kinetics similar to that of cecropin.
Drosophila attacin is most closely related to sarcotoxin II of
Sarcophaga peregrina, but it lacks the extra domains that are unique to this protein, and the overall domain structure of the
AttA gene product is identical to that of the attacins from
Hyalophora cecropia. |
| doi_str_mv | 10.1016/0965-1748(94)00091-C |
| format | Article |
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Drosophila. In this way, a cDNA clone for a member of the attacin family of antibacterial proteins was isolated. The corresponding
Attacin A (AttA) gene is localized at 51A-B on the second chromosome, and it is closely linked to at least one more cross-hybridizing gene. Injection of bacteria induces a 0.8 kb transcript, with expression kinetics similar to that of cecropin.
Drosophila attacin is most closely related to sarcotoxin II of
Sarcophaga peregrina, but it lacks the extra domains that are unique to this protein, and the overall domain structure of the
AttA gene product is identical to that of the attacins from
Hyalophora cecropia.</description><identifier>ISSN: 0965-1748</identifier><identifier>EISSN: 1879-0240</identifier><identifier>DOI: 10.1016/0965-1748(94)00091-C</identifier><identifier>PMID: 7742836</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Amino Acid Sequence ; amino acid sequences ; animal proteins ; Animals ; antibacterial proteins ; atta gene ; Attacin ; Base Sequence ; chromosome mapping ; Cloning, Molecular ; complementary DNA ; Differential display ; Diptera ; DNA, Complementary ; Drosophila ; Drosophila melanogaster ; Drosophila melanogaster - genetics ; Drosophila melanogaster - immunology ; Drosophila Proteins ; Drosophilidae ; Gene Expression ; Genes, Immediate-Early ; Genes, Insect ; immune response ; Insect Hormones - genetics ; Insect Hormones - metabolism ; Insect immunity ; Insect Proteins ; Kinetics ; messenger RNA ; Molecular Sequence Data ; nucleic acid hybridization ; nucleotide sequences ; polymerase chain reaction ; Polymerase Chain Reaction - methods ; polytene chromosomes ; Sequence Homology, Amino Acid ; structural genes</subject><ispartof>Insect biochemistry and molecular biology, 1995-04, Vol.25 (4), p.511-518</ispartof><rights>1995</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c478t-e4a885a72414295f46ac6b497cc98b1848c50c64985702637b7df0e8cec8a8923</citedby><cites>FETCH-LOGICAL-c478t-e4a885a72414295f46ac6b497cc98b1848c50c64985702637b7df0e8cec8a8923</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0965-1748(94)00091-C$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7742836$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Åsling, Bengt</creatorcontrib><creatorcontrib>Dushay, Mitchell S.</creatorcontrib><creatorcontrib>Hultmark, Dan</creatorcontrib><title>Identification of early genes in the Drosophila immune response by PCR-based differential display: the Attacin A gene and the evolution of attacin-like proteins</title><title>Insect biochemistry and molecular biology</title><addtitle>Insect Biochem Mol Biol</addtitle><description>We are using the PCR-based differential display technique to isolate genes which are induced during the immune response in
Drosophila. In this way, a cDNA clone for a member of the attacin family of antibacterial proteins was isolated. The corresponding
Attacin A (AttA) gene is localized at 51A-B on the second chromosome, and it is closely linked to at least one more cross-hybridizing gene. Injection of bacteria induces a 0.8 kb transcript, with expression kinetics similar to that of cecropin.
Drosophila attacin is most closely related to sarcotoxin II of
Sarcophaga peregrina, but it lacks the extra domains that are unique to this protein, and the overall domain structure of the
AttA gene product is identical to that of the attacins from
Hyalophora cecropia.</description><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>animal proteins</subject><subject>Animals</subject><subject>antibacterial proteins</subject><subject>atta gene</subject><subject>Attacin</subject><subject>Base Sequence</subject><subject>chromosome mapping</subject><subject>Cloning, Molecular</subject><subject>complementary DNA</subject><subject>Differential display</subject><subject>Diptera</subject><subject>DNA, Complementary</subject><subject>Drosophila</subject><subject>Drosophila melanogaster</subject><subject>Drosophila melanogaster - genetics</subject><subject>Drosophila melanogaster - immunology</subject><subject>Drosophila Proteins</subject><subject>Drosophilidae</subject><subject>Gene Expression</subject><subject>Genes, Immediate-Early</subject><subject>Genes, Insect</subject><subject>immune response</subject><subject>Insect Hormones - genetics</subject><subject>Insect Hormones - metabolism</subject><subject>Insect immunity</subject><subject>Insect Proteins</subject><subject>Kinetics</subject><subject>messenger RNA</subject><subject>Molecular Sequence Data</subject><subject>nucleic acid hybridization</subject><subject>nucleotide sequences</subject><subject>polymerase chain reaction</subject><subject>Polymerase Chain Reaction - methods</subject><subject>polytene chromosomes</subject><subject>Sequence Homology, Amino Acid</subject><subject>structural genes</subject><issn>0965-1748</issn><issn>1879-0240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9u1DAQhy0EKtvCG4DwCcEhYCdObPeAtEr5U6kSCOjZcpxxa0jsYCeV9m14VLybpUc4WfZ88401P4SeUfKGEtq8JbKpC8qZeCXZa0KIpEX7AG2o4LIgJSMP0eYeeYxOU_qRIcZqfoJOOGelqJoN-n3Zg5-ddUbPLngcLAYdhx2-AQ8JO4_nW8AXMaQw3bpBYzeOiwccIU3BJ8DdDn9pvxadTtDj3lkLcS_UQ76kadC784NhO8_aZNv2IMba94dnuAvD8newXplicD8BTzHM4Hx6gh5ZPSR4ejzP0PWH99_bT8XV54-X7faqMIyLuQCmhag1Lxllpawta7RpOia5MVJ0VDBhamIaJkXNSdlUvOO9JSAMGKGFLKsz9HL15sG_FkizGl0yMAzaQ1iS4jx3CV79F6SNEJxKlkG2giZvL0Wwaopu1HGnKFH7BNU-HrWPR0mmDgmqNrc9P_qXboT-vukYWa6_WOtWB6Vvokvq-ltJaEVoTSpZi0y8WwnI-7pzEFUyDryB3kUws-qD-_cX_gCB8rVn</recordid><startdate>19950401</startdate><enddate>19950401</enddate><creator>Åsling, Bengt</creator><creator>Dushay, Mitchell S.</creator><creator>Hultmark, Dan</creator><general>Elsevier Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19950401</creationdate><title>Identification of early genes in the Drosophila immune response by PCR-based differential display: the Attacin A gene and the evolution of attacin-like proteins</title><author>Åsling, Bengt ; Dushay, Mitchell S. ; Hultmark, Dan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c478t-e4a885a72414295f46ac6b497cc98b1848c50c64985702637b7df0e8cec8a8923</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Amino Acid Sequence</topic><topic>amino acid sequences</topic><topic>animal proteins</topic><topic>Animals</topic><topic>antibacterial proteins</topic><topic>atta gene</topic><topic>Attacin</topic><topic>Base Sequence</topic><topic>chromosome mapping</topic><topic>Cloning, Molecular</topic><topic>complementary DNA</topic><topic>Differential display</topic><topic>Diptera</topic><topic>DNA, Complementary</topic><topic>Drosophila</topic><topic>Drosophila melanogaster</topic><topic>Drosophila melanogaster - genetics</topic><topic>Drosophila melanogaster - immunology</topic><topic>Drosophila Proteins</topic><topic>Drosophilidae</topic><topic>Gene Expression</topic><topic>Genes, Immediate-Early</topic><topic>Genes, Insect</topic><topic>immune response</topic><topic>Insect Hormones - genetics</topic><topic>Insect Hormones - metabolism</topic><topic>Insect immunity</topic><topic>Insect Proteins</topic><topic>Kinetics</topic><topic>messenger RNA</topic><topic>Molecular Sequence Data</topic><topic>nucleic acid hybridization</topic><topic>nucleotide sequences</topic><topic>polymerase chain reaction</topic><topic>Polymerase Chain Reaction - methods</topic><topic>polytene chromosomes</topic><topic>Sequence Homology, Amino Acid</topic><topic>structural genes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Åsling, Bengt</creatorcontrib><creatorcontrib>Dushay, Mitchell S.</creatorcontrib><creatorcontrib>Hultmark, Dan</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Insect biochemistry and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Åsling, Bengt</au><au>Dushay, Mitchell S.</au><au>Hultmark, Dan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of early genes in the Drosophila immune response by PCR-based differential display: the Attacin A gene and the evolution of attacin-like proteins</atitle><jtitle>Insect biochemistry and molecular biology</jtitle><addtitle>Insect Biochem Mol Biol</addtitle><date>1995-04-01</date><risdate>1995</risdate><volume>25</volume><issue>4</issue><spage>511</spage><epage>518</epage><pages>511-518</pages><issn>0965-1748</issn><eissn>1879-0240</eissn><abstract>We are using the PCR-based differential display technique to isolate genes which are induced during the immune response in
Drosophila. In this way, a cDNA clone for a member of the attacin family of antibacterial proteins was isolated. The corresponding
Attacin A (AttA) gene is localized at 51A-B on the second chromosome, and it is closely linked to at least one more cross-hybridizing gene. Injection of bacteria induces a 0.8 kb transcript, with expression kinetics similar to that of cecropin.
Drosophila attacin is most closely related to sarcotoxin II of
Sarcophaga peregrina, but it lacks the extra domains that are unique to this protein, and the overall domain structure of the
AttA gene product is identical to that of the attacins from
Hyalophora cecropia.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>7742836</pmid><doi>10.1016/0965-1748(94)00091-C</doi><tpages>8</tpages></addata></record> |
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| ispartof | Insect biochemistry and molecular biology, 1995-04, Vol.25 (4), p.511-518 |
| issn | 0965-1748 1879-0240 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Amino Acid Sequence amino acid sequences animal proteins Animals antibacterial proteins atta gene Attacin Base Sequence chromosome mapping Cloning, Molecular complementary DNA Differential display Diptera DNA, Complementary Drosophila Drosophila melanogaster Drosophila melanogaster - genetics Drosophila melanogaster - immunology Drosophila Proteins Drosophilidae Gene Expression Genes, Immediate-Early Genes, Insect immune response Insect Hormones - genetics Insect Hormones - metabolism Insect immunity Insect Proteins Kinetics messenger RNA Molecular Sequence Data nucleic acid hybridization nucleotide sequences polymerase chain reaction Polymerase Chain Reaction - methods polytene chromosomes Sequence Homology, Amino Acid structural genes |
| title | Identification of early genes in the Drosophila immune response by PCR-based differential display: the Attacin A gene and the evolution of attacin-like proteins |
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