Homology Requirements for Ligation and Strand Exchange by the FLP Recombinase

The FLP recombinase of the 2-μm plasmid of Saccharomyces cerevisiae belongs to the integrase family whose members form a covalent bond between a conserved tyrosine of the recombinase and the 3′-phosphoryl group at the site of cleavage. Ligation takes place when the 5′-OH generated during the cleavag...

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