Plasticity of vascular smooth muscle alpha-actin gene transcription. Characterization of multiple, single-, and double-strand specific DNA- binding proteins in myoblasts and fibroblasts
Transcriptional activity of the mouse vascular smooth muscle (VSM) alpha-actin promoter was governed by both cell type and developmental stage-specific mechanisms. A purine-rich motif (PrM) located as â181 to â176 in the promoter was absolutely required for activation in mouse AKR-2B embryonic f...
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| Veröffentlicht in: | The Journal of biological chemistry 1995-05, Vol.270 (19), p.11310-11321 |
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| creator | Cogan, J G Sun, S Stoflet, E S Schmidt, L J Getz, M J Strauch, A R |
| description | Transcriptional activity of the mouse vascular smooth muscle (VSM) alpha-actin promoter was governed by both cell type and
developmental stage-specific mechanisms. A purine-rich motif (PrM) located as â181 to â176 in the promoter was absolutely
required for activation in mouse AKR-2B embryonic fibroblasts and partially contributed to activation in undifferentiated
mouse BC3H1 myoblasts. Transcriptional enhancer factor 1 recognized the PrM and cooperated with other promoter-binding proteins
to regulate serum growth factor-dependent transcription in both myoblasts and fibroblasts. Two distinct protein factors (VAC-ssBF1
and VAC-ssBF2) also were identified that bound sequence-specifically to single-stranded oligonucleotide probes that spanned
both the PrM and a closely positioned negative regulatory element. VAC-ssBF1 and BF2 binding activity was detected in undifferentiated
myoblasts, embryonic fibroblasts, and several smooth muscle tissues in the mouse and human. A myoblast-specific protein (VAC-RF1)
also was detected that bound double-stranded probes containing a CArG-like sequence that previously was shown to impart strong,
cell type specific repression. The binding activity of transcription enhancer factor 1, VAC-RF1, and VAC-ssBF1 was significantly
diminished when confluent BC3H1 myoblasts differentiated into myocytes and expressed VSM alpha-actin mRNA after exposure to
serum-free medium. The results indicated that cell type-specific control of the VSM alpha-actin gene promoter required the
participation of multiple DNA-binding proteins, including two that were enriched in smooth muscle and had preferential affinity
for single-stranded DNA. |
| format | Article |
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developmental stage-specific mechanisms. A purine-rich motif (PrM) located as â181 to â176 in the promoter was absolutely
required for activation in mouse AKR-2B embryonic fibroblasts and partially contributed to activation in undifferentiated
mouse BC3H1 myoblasts. Transcriptional enhancer factor 1 recognized the PrM and cooperated with other promoter-binding proteins
to regulate serum growth factor-dependent transcription in both myoblasts and fibroblasts. Two distinct protein factors (VAC-ssBF1
and VAC-ssBF2) also were identified that bound sequence-specifically to single-stranded oligonucleotide probes that spanned
both the PrM and a closely positioned negative regulatory element. VAC-ssBF1 and BF2 binding activity was detected in undifferentiated
myoblasts, embryonic fibroblasts, and several smooth muscle tissues in the mouse and human. A myoblast-specific protein (VAC-RF1)
also was detected that bound double-stranded probes containing a CArG-like sequence that previously was shown to impart strong,
cell type specific repression. The binding activity of transcription enhancer factor 1, VAC-RF1, and VAC-ssBF1 was significantly
diminished when confluent BC3H1 myoblasts differentiated into myocytes and expressed VSM alpha-actin mRNA after exposure to
serum-free medium. The results indicated that cell type-specific control of the VSM alpha-actin gene promoter required the
participation of multiple DNA-binding proteins, including two that were enriched in smooth muscle and had preferential affinity
for single-stranded DNA.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>PMID: 7744768</identifier><language>eng</language><publisher>United States: American Society for Biochemistry and Molecular Biology</publisher><subject>Actins - biosynthesis ; Actins - genetics ; Animals ; Base Sequence ; Cell Differentiation ; Cell Line ; Chloramphenicol O-Acetyltransferase - biosynthesis ; DNA-Binding Proteins - isolation & purification ; DNA-Binding Proteins - metabolism ; Embryo, Mammalian ; Fibroblasts - metabolism ; Gene Expression ; Mice ; Mice, Inbred AKR ; Molecular Sequence Data ; Muscles - cytology ; Muscles - metabolism ; Nuclear Proteins - isolation & purification ; Nuclear Proteins - metabolism ; Oligonucleotide Probes ; Promoter Regions, Genetic ; TATA Box ; Transcription, Genetic ; Transfection</subject><ispartof>The Journal of biological chemistry, 1995-05, Vol.270 (19), p.11310-11321</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7744768$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cogan, J G</creatorcontrib><creatorcontrib>Sun, S</creatorcontrib><creatorcontrib>Stoflet, E S</creatorcontrib><creatorcontrib>Schmidt, L J</creatorcontrib><creatorcontrib>Getz, M J</creatorcontrib><creatorcontrib>Strauch, A R</creatorcontrib><title>Plasticity of vascular smooth muscle alpha-actin gene transcription. Characterization of multiple, single-, and double-strand specific DNA- binding proteins in myoblasts and fibroblasts</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>Transcriptional activity of the mouse vascular smooth muscle (VSM) alpha-actin promoter was governed by both cell type and
developmental stage-specific mechanisms. A purine-rich motif (PrM) located as â181 to â176 in the promoter was absolutely
required for activation in mouse AKR-2B embryonic fibroblasts and partially contributed to activation in undifferentiated
mouse BC3H1 myoblasts. Transcriptional enhancer factor 1 recognized the PrM and cooperated with other promoter-binding proteins
to regulate serum growth factor-dependent transcription in both myoblasts and fibroblasts. Two distinct protein factors (VAC-ssBF1
and VAC-ssBF2) also were identified that bound sequence-specifically to single-stranded oligonucleotide probes that spanned
both the PrM and a closely positioned negative regulatory element. VAC-ssBF1 and BF2 binding activity was detected in undifferentiated
myoblasts, embryonic fibroblasts, and several smooth muscle tissues in the mouse and human. A myoblast-specific protein (VAC-RF1)
also was detected that bound double-stranded probes containing a CArG-like sequence that previously was shown to impart strong,
cell type specific repression. The binding activity of transcription enhancer factor 1, VAC-RF1, and VAC-ssBF1 was significantly
diminished when confluent BC3H1 myoblasts differentiated into myocytes and expressed VSM alpha-actin mRNA after exposure to
serum-free medium. The results indicated that cell type-specific control of the VSM alpha-actin gene promoter required the
participation of multiple DNA-binding proteins, including two that were enriched in smooth muscle and had preferential affinity
for single-stranded DNA.</description><subject>Actins - biosynthesis</subject><subject>Actins - genetics</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Cell Differentiation</subject><subject>Cell Line</subject><subject>Chloramphenicol O-Acetyltransferase - biosynthesis</subject><subject>DNA-Binding Proteins - isolation & purification</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Embryo, Mammalian</subject><subject>Fibroblasts - metabolism</subject><subject>Gene Expression</subject><subject>Mice</subject><subject>Mice, Inbred AKR</subject><subject>Molecular Sequence Data</subject><subject>Muscles - cytology</subject><subject>Muscles - metabolism</subject><subject>Nuclear Proteins - isolation & purification</subject><subject>Nuclear Proteins - metabolism</subject><subject>Oligonucleotide Probes</subject><subject>Promoter Regions, Genetic</subject><subject>TATA Box</subject><subject>Transcription, Genetic</subject><subject>Transfection</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUU2P1DAMrRCrZVj4CUg5IE5blI-mSY-rgV2QViwHkLhVaeJOjdKmJClo-Gf8OzLs3PHFst_z85P9pNoxqkUtJPv2tNpRylndcamfVc9T-k5LNB27rC6VahrV6l3157M3KaPFfCRhJD9Nsps3kaQ5hDyReUvWAzF-nUxtbMaFHGABkqNZko24ZgzLW7KfTCwoRPxtTp2T1Lz5jKuHa5JwOXior4lZHHFhG0qRTgqOpBUsjmjJu083NRlwcYVL1hgy4JJIWTcfw3CymP5NjzjEc_2iuhiNT_DynK-qr7fvv-w_1PcPdx_3N_f1xDTLNSgpmVGyYSApHxxvYRyA2pGbrhGO01aJcpO2s9BKxqQzCkbRaCc7TrV24qp686hbXP3YIOV-xmTBe7NA2FKvFG-51vS_RNYqKihVhfjqTNyGGVy_RpxNPPbnpxT89SM-4WH6hRH6AYOdYO65oj3resYEo-IvGQuY-A</recordid><startdate>19950512</startdate><enddate>19950512</enddate><creator>Cogan, J G</creator><creator>Sun, S</creator><creator>Stoflet, E S</creator><creator>Schmidt, L J</creator><creator>Getz, M J</creator><creator>Strauch, A R</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>19950512</creationdate><title>Plasticity of vascular smooth muscle alpha-actin gene transcription. Characterization of multiple, single-, and double-strand specific DNA- binding proteins in myoblasts and fibroblasts</title><author>Cogan, J G ; Sun, S ; Stoflet, E S ; Schmidt, L J ; Getz, M J ; Strauch, A R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h181t-e7551a7541e502bd26efbe0cf2a943d2067349169ce65115da7ef348d592088d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Actins - biosynthesis</topic><topic>Actins - genetics</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Cell Differentiation</topic><topic>Cell Line</topic><topic>Chloramphenicol O-Acetyltransferase - biosynthesis</topic><topic>DNA-Binding Proteins - isolation & purification</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Embryo, Mammalian</topic><topic>Fibroblasts - metabolism</topic><topic>Gene Expression</topic><topic>Mice</topic><topic>Mice, Inbred AKR</topic><topic>Molecular Sequence Data</topic><topic>Muscles - cytology</topic><topic>Muscles - metabolism</topic><topic>Nuclear Proteins - isolation & purification</topic><topic>Nuclear Proteins - metabolism</topic><topic>Oligonucleotide Probes</topic><topic>Promoter Regions, Genetic</topic><topic>TATA Box</topic><topic>Transcription, Genetic</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cogan, J G</creatorcontrib><creatorcontrib>Sun, S</creatorcontrib><creatorcontrib>Stoflet, E S</creatorcontrib><creatorcontrib>Schmidt, L J</creatorcontrib><creatorcontrib>Getz, M J</creatorcontrib><creatorcontrib>Strauch, A R</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cogan, J G</au><au>Sun, S</au><au>Stoflet, E S</au><au>Schmidt, L J</au><au>Getz, M J</au><au>Strauch, A R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Plasticity of vascular smooth muscle alpha-actin gene transcription. Characterization of multiple, single-, and double-strand specific DNA- binding proteins in myoblasts and fibroblasts</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1995-05-12</date><risdate>1995</risdate><volume>270</volume><issue>19</issue><spage>11310</spage><epage>11321</epage><pages>11310-11321</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>Transcriptional activity of the mouse vascular smooth muscle (VSM) alpha-actin promoter was governed by both cell type and
developmental stage-specific mechanisms. A purine-rich motif (PrM) located as â181 to â176 in the promoter was absolutely
required for activation in mouse AKR-2B embryonic fibroblasts and partially contributed to activation in undifferentiated
mouse BC3H1 myoblasts. Transcriptional enhancer factor 1 recognized the PrM and cooperated with other promoter-binding proteins
to regulate serum growth factor-dependent transcription in both myoblasts and fibroblasts. Two distinct protein factors (VAC-ssBF1
and VAC-ssBF2) also were identified that bound sequence-specifically to single-stranded oligonucleotide probes that spanned
both the PrM and a closely positioned negative regulatory element. VAC-ssBF1 and BF2 binding activity was detected in undifferentiated
myoblasts, embryonic fibroblasts, and several smooth muscle tissues in the mouse and human. A myoblast-specific protein (VAC-RF1)
also was detected that bound double-stranded probes containing a CArG-like sequence that previously was shown to impart strong,
cell type specific repression. The binding activity of transcription enhancer factor 1, VAC-RF1, and VAC-ssBF1 was significantly
diminished when confluent BC3H1 myoblasts differentiated into myocytes and expressed VSM alpha-actin mRNA after exposure to
serum-free medium. The results indicated that cell type-specific control of the VSM alpha-actin gene promoter required the
participation of multiple DNA-binding proteins, including two that were enriched in smooth muscle and had preferential affinity
for single-stranded DNA.</abstract><cop>United States</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>7744768</pmid><tpages>12</tpages></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Actins - biosynthesis Actins - genetics Animals Base Sequence Cell Differentiation Cell Line Chloramphenicol O-Acetyltransferase - biosynthesis DNA-Binding Proteins - isolation & purification DNA-Binding Proteins - metabolism Embryo, Mammalian Fibroblasts - metabolism Gene Expression Mice Mice, Inbred AKR Molecular Sequence Data Muscles - cytology Muscles - metabolism Nuclear Proteins - isolation & purification Nuclear Proteins - metabolism Oligonucleotide Probes Promoter Regions, Genetic TATA Box Transcription, Genetic Transfection |
| title | Plasticity of vascular smooth muscle alpha-actin gene transcription. Characterization of multiple, single-, and double-strand specific DNA- binding proteins in myoblasts and fibroblasts |
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