Conjugation to preactivated proteins using divinylsulfone and iodoacetic acid

Two methods for the preactivation of proteins and conjugation of peptides to proteins under mild conditions are presented. Preactivation of proteins with divinylsulfone (DVS) permits peptide conjugation through either amino, hydroxyl or sulphydryl groups depending on the coupling pH used, while prea...

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Veröffentlicht in:	Journal of immunological methods 1995-04, Vol.181 (2), p.187-200
Hauptverfasser:	Houen, Gunnar, Jensen, Ole Majgaard
Format:	Artikel
Sprache:	eng
Schlagworte:	Amines - chemistry Amino Acid Sequence Antigens - chemistry Biological and medical sciences Carrier Conjugation Fundamental and applied biological sciences. Psychology Fundamental immunology Hydroxides - chemistry Immunoassay - methods Immunoconjugates - chemistry Iodoacetates - chemistry Iodoacetic Acid Molecular immunology Molecular Sequence Data Peptide conjugation Protein activation Proteins - chemistry Sulfhydryl Compounds - chemistry Sulfones - chemistry Techniques
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