Structural changes induced in capillary endothelial cells by growth factors: Altered distribution of cytoskeletal elements and organelles in cells spreading in the presence of tumor conditioned medium and hypothalamus derived growth factor

Using high-voltage and conventional electron microscopy of cell whole mounts, we have investigated the effects of tumor-conditioned medium and hypothalmus-derived growth factor on the structure of capillary endothelial cells during their attachment and spreading in tissue culture. Cells were culture...

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Veröffentlicht in:Tissue & cell 1986, Vol.18 (6), p.827-837
Hauptverfasser: Dethlefsen, Sandra M., Butterfield, Catherine E., Ausprunk, Dianna H.
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container_title Tissue & cell
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creator Dethlefsen, Sandra M.
Butterfield, Catherine E.
Ausprunk, Dianna H.
description Using high-voltage and conventional electron microscopy of cell whole mounts, we have investigated the effects of tumor-conditioned medium and hypothalmus-derived growth factor on the structure of capillary endothelial cells during their attachment and spreading in tissue culture. Cells were cultured in A, Dulbecco's Modified Eagle's Medium (DMEM) and 10% calf serum; B, equal parts of A and 48 hr mouse sarcoma conditioned medium; and C, A containing 10 units of hypothalamus-derived growth factor. Cells cultured in all three media were fully spread, and to the same extent, by 4 hr after plating. While spreading, cells cultured in DMEM alone developed prominent stress fibers and contained numerous bundles of microtubules which formed radical tracts along which mitochondria and other organelles rapidly moved to the cell periphery. Stress fibers were thinner and microtubule tracts fewer in number in cells cultured in tumor-conditioned medium. In 4 hr, organelles moved only part of the distance to the cell margin. Stress fibers were rudimentary and microtubules randomly orientated in cells exposed to hypothalamus-derived growth factor. Most organelles remained near the cell nucleus. The dramatic decrease in stress fibers and microtubule tracts in cells grown in tumorconditioned medium and hypothalamus-derived growth factor and the subsequent decreased capacity of the cells to move organelles toward their periphery could have some functional significance relative to the growth-promoting activity of these substances.
doi_str_mv 10.1016/0040-8166(86)90041-8
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subjects Adrenal Glands - blood supply
Animals
Biological and medical sciences
Capillaries - drug effects
Capillaries - ultrastructure
Cattle
Cell morphology
Culture Media
cytoskeleton
Cytoskeleton - drug effects
Cytoskeleton - ultrastructure
Endothelial cells
Endothelium - drug effects
Endothelium - ultrastructure
Fundamental and applied biological sciences. Psychology
growth factors
Growth Substances - pharmacology
Hypothalamus - physiology
Mice
Microscopy, Electron
Molecular and cellular biology
organelles
Organoids - drug effects
Organoids - ultrastructure
Proteins
Sarcoma, Experimental - pathology
title Structural changes induced in capillary endothelial cells by growth factors: Altered distribution of cytoskeletal elements and organelles in cells spreading in the presence of tumor conditioned medium and hypothalamus derived growth factor
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