Differentiation between glycoprotein III gene-deleted vaccine and wild-type strains of pseudorabies virus by polymerase chain reaction (PCR)
One of the attenuated and genetically recombinant modified-live viral (MLV) vaccine strains currently used contains a deletion in its glycoprotein III ( gIII) gene, while prototypic wild-type pseudorabies (WT-PR) viruses contain an intact gIII gene. A polymerase chain reaction (PCR) system different...
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| Veröffentlicht in: | Journal of virological methods 1995-02, Vol.51 (2), p.267-276 |
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| creator | Ishikawa, Kiyoyasu Jin-yama, Mariko Saitoh, Akito Takagi, masami Muramatsu, Masatake Itoh, Osamu |
| description | One of the attenuated and genetically recombinant modified-live viral (MLV) vaccine strains currently used contains a deletion in its glycoprotein III (
gIII) gene, while prototypic wild-type pseudorabies (WT-PR) viruses contain an intact
gIII gene. A polymerase chain reaction (PCR) system differentiating, based on this difference, between the vaccine virus and prototypic WT-PR viruses was investigated. This PCR system utilized two consecutive stages. Primers for the first-stage PCR were designed so as to amplify of DNA fragments lengths in respect to the vaccine and WT-PR viruses. The second-stage PCR amplification for improving the sensitivity and specificity and for confirming of the sites deleted from the first-stage PCR products produced an all-or-none result: internal DNA fragments were derived from only WT-PR viruses but not from the vaccine virus. These PCR-amplified fragment length polymorphisms clearly distinguished the vaccine virus from WT-PR viruses. The vaccine and WT-PR viruses in mixtures were each identified in this PCR system. This PCR system may permit rapid and sensitive detection of PR viral
gIII gene, analysis of the genotype of PR virus isolates, and also examination of the isolates for purity and identity. |
| doi_str_mv | 10.1016/0166-0934(94)00115-W |
| format | Article |
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gIII) gene, while prototypic wild-type pseudorabies (WT-PR) viruses contain an intact
gIII gene. A polymerase chain reaction (PCR) system differentiating, based on this difference, between the vaccine virus and prototypic WT-PR viruses was investigated. This PCR system utilized two consecutive stages. Primers for the first-stage PCR were designed so as to amplify of DNA fragments lengths in respect to the vaccine and WT-PR viruses. The second-stage PCR amplification for improving the sensitivity and specificity and for confirming of the sites deleted from the first-stage PCR products produced an all-or-none result: internal DNA fragments were derived from only WT-PR viruses but not from the vaccine virus. These PCR-amplified fragment length polymorphisms clearly distinguished the vaccine virus from WT-PR viruses. The vaccine and WT-PR viruses in mixtures were each identified in this PCR system. This PCR system may permit rapid and sensitive detection of PR viral
gIII gene, analysis of the genotype of PR virus isolates, and also examination of the isolates for purity and identity.</description><identifier>ISSN: 0166-0934</identifier><identifier>EISSN: 1879-0984</identifier><identifier>DOI: 10.1016/0166-0934(94)00115-W</identifier><identifier>PMID: 7738147</identifier><identifier>CODEN: JVMEDH</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Base Sequence ; Biological and medical sciences ; DNA Primers ; Fundamental and applied biological sciences. Psychology ; Glycoprotein III ; Herpesvirus 1, Suid - genetics ; Microbiology ; Molecular Sequence Data ; PCR ; Polymerase Chain Reaction - methods ; Polymorphism, Restriction Fragment Length ; Pseudorabies Vaccines ; Pseudorabies viras ; pseudorabies virus ; Sensitivity and Specificity ; Sequence Deletion - genetics ; Techniques used in virology ; Vaccine ; Viral Envelope Proteins - genetics ; Viral Vaccines - genetics ; Virology</subject><ispartof>Journal of virological methods, 1995-02, Vol.51 (2), p.267-276</ispartof><rights>1995</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-c5014c415407fcaf73142bc2d09c335d8eb90bfc8863a27b9d79e076f55cd9a73</citedby><cites>FETCH-LOGICAL-c417t-c5014c415407fcaf73142bc2d09c335d8eb90bfc8863a27b9d79e076f55cd9a73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0166-0934(94)00115-W$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3417963$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7738147$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ishikawa, Kiyoyasu</creatorcontrib><creatorcontrib>Jin-yama, Mariko</creatorcontrib><creatorcontrib>Saitoh, Akito</creatorcontrib><creatorcontrib>Takagi, masami</creatorcontrib><creatorcontrib>Muramatsu, Masatake</creatorcontrib><creatorcontrib>Itoh, Osamu</creatorcontrib><title>Differentiation between glycoprotein III gene-deleted vaccine and wild-type strains of pseudorabies virus by polymerase chain reaction (PCR)</title><title>Journal of virological methods</title><addtitle>J Virol Methods</addtitle><description>One of the attenuated and genetically recombinant modified-live viral (MLV) vaccine strains currently used contains a deletion in its glycoprotein III (
gIII) gene, while prototypic wild-type pseudorabies (WT-PR) viruses contain an intact
gIII gene. A polymerase chain reaction (PCR) system differentiating, based on this difference, between the vaccine virus and prototypic WT-PR viruses was investigated. This PCR system utilized two consecutive stages. Primers for the first-stage PCR were designed so as to amplify of DNA fragments lengths in respect to the vaccine and WT-PR viruses. The second-stage PCR amplification for improving the sensitivity and specificity and for confirming of the sites deleted from the first-stage PCR products produced an all-or-none result: internal DNA fragments were derived from only WT-PR viruses but not from the vaccine virus. These PCR-amplified fragment length polymorphisms clearly distinguished the vaccine virus from WT-PR viruses. The vaccine and WT-PR viruses in mixtures were each identified in this PCR system. This PCR system may permit rapid and sensitive detection of PR viral
gIII gene, analysis of the genotype of PR virus isolates, and also examination of the isolates for purity and identity.</description><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>DNA Primers</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycoprotein III</subject><subject>Herpesvirus 1, Suid - genetics</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>PCR</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Pseudorabies Vaccines</subject><subject>Pseudorabies viras</subject><subject>pseudorabies virus</subject><subject>Sensitivity and Specificity</subject><subject>Sequence Deletion - genetics</subject><subject>Techniques used in virology</subject><subject>Vaccine</subject><subject>Viral Envelope Proteins - genetics</subject><subject>Viral Vaccines - genetics</subject><subject>Virology</subject><issn>0166-0934</issn><issn>1879-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2OFCEQgInRrOPoG2jCwZjdQys0dNNcTMz4N8kmGqPZI6GhWDE9dAv0bPodfGiZnckc9UAg1FdVUB9Czyl5TQlt35TVVkQyfin5FSGUNtXNA7SinZDluuMP0eqMPEZPUvpFCGkEYxfoQgjWUS5W6M977xxECNnr7MeAe8h3AAHfDosZpzhm8AFvt1t8CwEqCwNksHivjfEBsA4W3_nBVnmZAKcctQ8Jjw5PCWY7Rt17SHjv45xwv-BpHJYdRJ0Am58FxRG0uW97-XXz7eopeuT0kODZaV-jHx8_fN98rq6_fNpu3l1XhlORK9MQysux4UQ4o51glNe9qS2RhrHGdtBL0jvTdS3TteilFRKIaF3TGCu1YGv06li3_O_3DCmrnU8GhkEHGOekhKgbLlj9X5C2XUsO6BrxI2jimFIEp6bodzouihJ1sKUOKtRBhZJc3dtSNyXtxan-3O_AnpNOekr85Smuk9GDizoYn84YK-OQLSvY2yMGZWh7D1El4yEYsD6CycqO_t_v-AszKLKi</recordid><startdate>19950201</startdate><enddate>19950201</enddate><creator>Ishikawa, Kiyoyasu</creator><creator>Jin-yama, Mariko</creator><creator>Saitoh, Akito</creator><creator>Takagi, masami</creator><creator>Muramatsu, Masatake</creator><creator>Itoh, Osamu</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19950201</creationdate><title>Differentiation between glycoprotein III gene-deleted vaccine and wild-type strains of pseudorabies virus by polymerase chain reaction (PCR)</title><author>Ishikawa, Kiyoyasu ; Jin-yama, Mariko ; Saitoh, Akito ; Takagi, masami ; Muramatsu, Masatake ; Itoh, Osamu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-c5014c415407fcaf73142bc2d09c335d8eb90bfc8863a27b9d79e076f55cd9a73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>DNA Primers</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glycoprotein III</topic><topic>Herpesvirus 1, Suid - genetics</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>PCR</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Pseudorabies Vaccines</topic><topic>Pseudorabies viras</topic><topic>pseudorabies virus</topic><topic>Sensitivity and Specificity</topic><topic>Sequence Deletion - genetics</topic><topic>Techniques used in virology</topic><topic>Vaccine</topic><topic>Viral Envelope Proteins - genetics</topic><topic>Viral Vaccines - genetics</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ishikawa, Kiyoyasu</creatorcontrib><creatorcontrib>Jin-yama, Mariko</creatorcontrib><creatorcontrib>Saitoh, Akito</creatorcontrib><creatorcontrib>Takagi, masami</creatorcontrib><creatorcontrib>Muramatsu, Masatake</creatorcontrib><creatorcontrib>Itoh, Osamu</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of virological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ishikawa, Kiyoyasu</au><au>Jin-yama, Mariko</au><au>Saitoh, Akito</au><au>Takagi, masami</au><au>Muramatsu, Masatake</au><au>Itoh, Osamu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differentiation between glycoprotein III gene-deleted vaccine and wild-type strains of pseudorabies virus by polymerase chain reaction (PCR)</atitle><jtitle>Journal of virological methods</jtitle><addtitle>J Virol Methods</addtitle><date>1995-02-01</date><risdate>1995</risdate><volume>51</volume><issue>2</issue><spage>267</spage><epage>276</epage><pages>267-276</pages><issn>0166-0934</issn><eissn>1879-0984</eissn><coden>JVMEDH</coden><abstract>One of the attenuated and genetically recombinant modified-live viral (MLV) vaccine strains currently used contains a deletion in its glycoprotein III (
gIII) gene, while prototypic wild-type pseudorabies (WT-PR) viruses contain an intact
gIII gene. A polymerase chain reaction (PCR) system differentiating, based on this difference, between the vaccine virus and prototypic WT-PR viruses was investigated. This PCR system utilized two consecutive stages. Primers for the first-stage PCR were designed so as to amplify of DNA fragments lengths in respect to the vaccine and WT-PR viruses. The second-stage PCR amplification for improving the sensitivity and specificity and for confirming of the sites deleted from the first-stage PCR products produced an all-or-none result: internal DNA fragments were derived from only WT-PR viruses but not from the vaccine virus. These PCR-amplified fragment length polymorphisms clearly distinguished the vaccine virus from WT-PR viruses. The vaccine and WT-PR viruses in mixtures were each identified in this PCR system. This PCR system may permit rapid and sensitive detection of PR viral
gIII gene, analysis of the genotype of PR virus isolates, and also examination of the isolates for purity and identity.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>7738147</pmid><doi>10.1016/0166-0934(94)00115-W</doi><tpages>10</tpages></addata></record> |
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| subjects | Base Sequence Biological and medical sciences DNA Primers Fundamental and applied biological sciences. Psychology Glycoprotein III Herpesvirus 1, Suid - genetics Microbiology Molecular Sequence Data PCR Polymerase Chain Reaction - methods Polymorphism, Restriction Fragment Length Pseudorabies Vaccines Pseudorabies viras pseudorabies virus Sensitivity and Specificity Sequence Deletion - genetics Techniques used in virology Vaccine Viral Envelope Proteins - genetics Viral Vaccines - genetics Virology |
| title | Differentiation between glycoprotein III gene-deleted vaccine and wild-type strains of pseudorabies virus by polymerase chain reaction (PCR) |
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