Quantitative Determination of Human Plasma Apolipoprotein A-II by a Non Competitive Enzyme-Linked Immunosorbent Assay
A noncompetitive enzyme-linked immunosorbent assay (ELISA) for apolipoprotein A-II (ApoA-II) was developed. Microtiter plates were coated with affinity purified antibodies to ApoA-II. After incubation with human plasma, the amount of ApoA-II bound to the coated plate was determined with peroxidase-l...
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| Veröffentlicht in: | Journal of immunoassay (Monticello, N.Y.) N.Y.), 1986-01, Vol.7 (4), p.285-307 |
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| container_end_page | 307 |
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| container_issue | 4 |
| container_start_page | 285 |
| container_title | Journal of immunoassay (Monticello, N.Y.) |
| container_volume | 7 |
| creator | Puchois, P. Kandoussi, A. Duriez, P. Fruchart, J. C. McConathy, W. J. Koren, E. |
| description | A noncompetitive enzyme-linked immunosorbent assay (ELISA) for apolipoprotein A-II (ApoA-II) was developed. Microtiter plates were coated with affinity purified antibodies to ApoA-II. After incubation with human plasma, the amount of ApoA-II bound to the coated plate was determined with peroxidase-labeled antibodies to ApoA-II. When pure ApoA-II or delipidated reference plasma was used as standard, a single step delipidation was required in order to unmask some antigenic sites of ApoA-II. However, the understimated ApoA-II values in untreated samples were shown to be corrected by using intact reference plasma as secondary standard. The average concentration of ApoA-II in normolipidemic plasma was 0,376 g/1. |
| doi_str_mv | 10.1080/01971528608060473 |
| format | Article |
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The average concentration of ApoA-II in normolipidemic plasma was 0,376 g/1.</description><identifier>ISSN: 0197-1522</identifier><identifier>DOI: 10.1080/01971528608060473</identifier><identifier>PMID: 3100579</identifier><language>eng</language><publisher>United States: Taylor & Francis Group</publisher><subject>Antibodies - isolation & purification ; Apolipoprotein A-II ; Apolipoproteins A - blood ; Apolipoproteins A - isolation & purification ; Buffers ; Enzyme-Linked Immunosorbent Assay - methods ; Human plasma ; Humans ; Hydrogen-Ion Concentration ; Hyperlipoproteinemia Type IV - blood ; Noncompetitive enzyme-linked immunosorbent assay ; Reference Values</subject><ispartof>Journal of immunoassay (Monticello, N.Y.), 1986-01, Vol.7 (4), p.285-307</ispartof><rights>Copyright Taylor & Francis Group, LLC 1986</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c380t-323908a79862e361ed89c29a3933cb69e7c1f3277d7b6a3649acb2f8e8acbc6d3</citedby><cites>FETCH-LOGICAL-c380t-323908a79862e361ed89c29a3933cb69e7c1f3277d7b6a3649acb2f8e8acbc6d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/01971528608060473$$EPDF$$P50$$Ginformaworld$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/01971528608060473$$EHTML$$P50$$Ginformaworld$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,59620,60409</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3100579$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Puchois, P.</creatorcontrib><creatorcontrib>Kandoussi, A.</creatorcontrib><creatorcontrib>Duriez, P.</creatorcontrib><creatorcontrib>Fruchart, J. 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The average concentration of ApoA-II in normolipidemic plasma was 0,376 g/1.</description><subject>Antibodies - isolation & purification</subject><subject>Apolipoprotein A-II</subject><subject>Apolipoproteins A - blood</subject><subject>Apolipoproteins A - isolation & purification</subject><subject>Buffers</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Human plasma</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Hyperlipoproteinemia Type IV - blood</subject><subject>Noncompetitive enzyme-linked immunosorbent assay</subject><subject>Reference Values</subject><issn>0197-1522</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1r3DAQhnVoSUOaH9BDQafc3EqWVx_Qy7JJm4UlH5CejSyPQa0lOZLc4Pz6aNkll1AyMAwz887DwIvQF0q-USLJd0KVoKta8tJw0gj2AZ3uZ1UZ1p_QeUp_SAnVkEbyE3TCKCEroU7RfD9rn23W2f4DfAkZorO-dMHjMODr2WmP70adnMbrKYx2ClMMGazH62q7xd2CNb4p4k1wExTQHnPlnxcH1c76v9DjrXOzDynEDnzG65T08hl9HPSY4PxYz9Dvn1cPm-tqd_tru1nvKsMkyRWrmSJSCyV5DYxT6KUytdJMMWY6rkAYOrBaiF50XDPeKG26epAgSzW8Z2fo4sAtPz_OkHLrbDIwjtpDmFMrRL1irOR7QtqIRhLGi5AehCaGlCIM7RSt03FpKWn3VrRvrCg3X4_wuXPQv14cTSj7H4e99UOITj-FOPZt1ssY4hC1Nzbttf_DvwAK0ZkY</recordid><startdate>19860101</startdate><enddate>19860101</enddate><creator>Puchois, P.</creator><creator>Kandoussi, A.</creator><creator>Duriez, P.</creator><creator>Fruchart, J. 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J.</au><au>Koren, E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantitative Determination of Human Plasma Apolipoprotein A-II by a Non Competitive Enzyme-Linked Immunosorbent Assay</atitle><jtitle>Journal of immunoassay (Monticello, N.Y.)</jtitle><addtitle>J Immunoassay</addtitle><date>1986-01-01</date><risdate>1986</risdate><volume>7</volume><issue>4</issue><spage>285</spage><epage>307</epage><pages>285-307</pages><issn>0197-1522</issn><abstract>A noncompetitive enzyme-linked immunosorbent assay (ELISA) for apolipoprotein A-II (ApoA-II) was developed. Microtiter plates were coated with affinity purified antibodies to ApoA-II. After incubation with human plasma, the amount of ApoA-II bound to the coated plate was determined with peroxidase-labeled antibodies to ApoA-II. When pure ApoA-II or delipidated reference plasma was used as standard, a single step delipidation was required in order to unmask some antigenic sites of ApoA-II. However, the understimated ApoA-II values in untreated samples were shown to be corrected by using intact reference plasma as secondary standard. The average concentration of ApoA-II in normolipidemic plasma was 0,376 g/1.</abstract><cop>United States</cop><pub>Taylor & Francis Group</pub><pmid>3100579</pmid><doi>10.1080/01971528608060473</doi><tpages>23</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0197-1522 |
| ispartof | Journal of immunoassay (Monticello, N.Y.), 1986-01, Vol.7 (4), p.285-307 |
| issn | 0197-1522 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77253325 |
| source | Taylor & Francis:Master (3349 titles); MEDLINE |
| subjects | Antibodies - isolation & purification Apolipoprotein A-II Apolipoproteins A - blood Apolipoproteins A - isolation & purification Buffers Enzyme-Linked Immunosorbent Assay - methods Human plasma Humans Hydrogen-Ion Concentration Hyperlipoproteinemia Type IV - blood Noncompetitive enzyme-linked immunosorbent assay Reference Values |
| title | Quantitative Determination of Human Plasma Apolipoprotein A-II by a Non Competitive Enzyme-Linked Immunosorbent Assay |
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