Cytosolic calcium facilitates release of secretory products after exocytotic vesicle fusion
We monitored single vesicle exocytosis by simultaneous measurements of cell membrane capacitance as an indicator of fusion and amperometric detection of serotonin release. We show here that vesicle-plasma membrane fusion in rat mast cell granules is followed by a variable, exponentially distributed,...
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| Veröffentlicht in: | FEBS letters 1995-04, Vol.363 (3), p.221-225 |
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| container_title | FEBS letters |
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| creator | Fernández-Chacón, Rafael de Toledo, Guillermo Alvarez |
| description | We monitored single vesicle exocytosis by simultaneous measurements of cell membrane capacitance as an indicator of fusion and amperometric detection of serotonin release. We show here that vesicle-plasma membrane fusion in rat mast cell granules is followed by a variable, exponentially distributed, delay before bulk release. This delay reflects the time required for the expansion of the exocytotic fusion pore, lasting, on average, 231 ms in resting cytosolic calcium, [Ca
2+]
i (50 nM). In the presence of [Ca
2+]
i in the low micromollar range, the lag between fusion and release was reduced to 123 ms. The characteristics of the amperometric signals were unchanged by [Ca
2+]
i. These results show a novel site of regulation in the exocytotic process, the fusion pore, which may represent a different mechanism facilitating transmitter release. |
| doi_str_mv | 10.1016/0014-5793(95)00319-5 |
| format | Article |
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2+]
i (50 nM). In the presence of [Ca
2+]
i in the low micromollar range, the lag between fusion and release was reduced to 123 ms. The characteristics of the amperometric signals were unchanged by [Ca
2+]
i. These results show a novel site of regulation in the exocytotic process, the fusion pore, which may represent a different mechanism facilitating transmitter release.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(95)00319-5</identifier><identifier>PMID: 7737406</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Amperometry ; Animals ; Calcium - physiology ; Cell capacitance ; Cell Degranulation ; Cytosol - physiology ; EGTA ; ethylene glycol-bis(β-aminoethyl ether) N,N,N′,N′-tetraacetic ; Exocytosis ; HEPES ; Mast Cells - physiology ; Membrane Fusion ; Membrane Potentials ; Mice ; Mice, Mutant Strains ; N-[2-hydroxyethyl]piperazine-N′-[2-ethanesulfo nic acid), GTPγS, guanosine-5′-O-(3-thiotriphosphate ; Patch-Clamp Techniques ; Rats ; Serotonin - metabolism ; Transmitter release</subject><ispartof>FEBS letters, 1995-04, Vol.363 (3), p.221-225</ispartof><rights>1995</rights><rights>FEBS Letters 363 (1995) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4865-a8187eee272a75697c9d2982b11702e96f975ccba98569245c9d4cf0d99501d3</citedby><cites>FETCH-LOGICAL-c4865-a8187eee272a75697c9d2982b11702e96f975ccba98569245c9d4cf0d99501d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0014-5793(95)00319-5$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>315,781,785,3551,27926,27927,45997</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7737406$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fernández-Chacón, Rafael</creatorcontrib><creatorcontrib>de Toledo, Guillermo Alvarez</creatorcontrib><title>Cytosolic calcium facilitates release of secretory products after exocytotic vesicle fusion</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>We monitored single vesicle exocytosis by simultaneous measurements of cell membrane capacitance as an indicator of fusion and amperometric detection of serotonin release. We show here that vesicle-plasma membrane fusion in rat mast cell granules is followed by a variable, exponentially distributed, delay before bulk release. This delay reflects the time required for the expansion of the exocytotic fusion pore, lasting, on average, 231 ms in resting cytosolic calcium, [Ca
2+]
i (50 nM). In the presence of [Ca
2+]
i in the low micromollar range, the lag between fusion and release was reduced to 123 ms. The characteristics of the amperometric signals were unchanged by [Ca
2+]
i. These results show a novel site of regulation in the exocytotic process, the fusion pore, which may represent a different mechanism facilitating transmitter release.</description><subject>Amperometry</subject><subject>Animals</subject><subject>Calcium - physiology</subject><subject>Cell capacitance</subject><subject>Cell Degranulation</subject><subject>Cytosol - physiology</subject><subject>EGTA</subject><subject>ethylene glycol-bis(β-aminoethyl ether) N,N,N′,N′-tetraacetic</subject><subject>Exocytosis</subject><subject>HEPES</subject><subject>Mast Cells - physiology</subject><subject>Membrane Fusion</subject><subject>Membrane Potentials</subject><subject>Mice</subject><subject>Mice, Mutant Strains</subject><subject>N-[2-hydroxyethyl]piperazine-N′-[2-ethanesulfo nic acid), GTPγS, guanosine-5′-O-(3-thiotriphosphate</subject><subject>Patch-Clamp Techniques</subject><subject>Rats</subject><subject>Serotonin - metabolism</subject><subject>Transmitter release</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkM1OxCAUhYnR6PjzBpqwMrqoQltK2ZjoxFETEzfuXBDm9jbBMIMCVeftpc7EpXFFuOfcj8Mh5JizC854c8kYrwshVXWmxDljFVeF2CIT3sqqqOqm3SaTX8se2Y_xleV7y9Uu2ZWykjVrJuRluko-emeBgnFghwXtDVhnk0kYaUCHJiL1PY0IAZMPK_oWfDdAitT0CQPFLw8ZkjLiA6MFh7QfovXLQ7LTGxfxaHMekOfZ7fP0vnh8unuYXj8WULeNKEybIyNiKUsjRaMkqK5UbTnnXLISVdMrKQDmRrVZLWuR9Rp61iklGO-qA3K6xuZc7wPGpBc2AjpnluiHqKUsRcVlm4312gjBxxiw12_BLkxYac70WKke-9JjX1oJ_VOpFnntZMMf5gvsfpc2HWZ9ttY_rcPVv5h6dntTjsI4z78Yp-NDV2sQ5rI-LAYdweISsLMBIenO27-TfgP8LJmu</recordid><startdate>19950424</startdate><enddate>19950424</enddate><creator>Fernández-Chacón, Rafael</creator><creator>de Toledo, Guillermo Alvarez</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19950424</creationdate><title>Cytosolic calcium facilitates release of secretory products after exocytotic vesicle fusion</title><author>Fernández-Chacón, Rafael ; de Toledo, Guillermo Alvarez</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4865-a8187eee272a75697c9d2982b11702e96f975ccba98569245c9d4cf0d99501d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Amperometry</topic><topic>Animals</topic><topic>Calcium - physiology</topic><topic>Cell capacitance</topic><topic>Cell Degranulation</topic><topic>Cytosol - physiology</topic><topic>EGTA</topic><topic>ethylene glycol-bis(β-aminoethyl ether) N,N,N′,N′-tetraacetic</topic><topic>Exocytosis</topic><topic>HEPES</topic><topic>Mast Cells - physiology</topic><topic>Membrane Fusion</topic><topic>Membrane Potentials</topic><topic>Mice</topic><topic>Mice, Mutant Strains</topic><topic>N-[2-hydroxyethyl]piperazine-N′-[2-ethanesulfo nic acid), GTPγS, guanosine-5′-O-(3-thiotriphosphate</topic><topic>Patch-Clamp Techniques</topic><topic>Rats</topic><topic>Serotonin - metabolism</topic><topic>Transmitter release</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fernández-Chacón, Rafael</creatorcontrib><creatorcontrib>de Toledo, Guillermo Alvarez</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fernández-Chacón, Rafael</au><au>de Toledo, Guillermo Alvarez</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cytosolic calcium facilitates release of secretory products after exocytotic vesicle fusion</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1995-04-24</date><risdate>1995</risdate><volume>363</volume><issue>3</issue><spage>221</spage><epage>225</epage><pages>221-225</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>We monitored single vesicle exocytosis by simultaneous measurements of cell membrane capacitance as an indicator of fusion and amperometric detection of serotonin release. We show here that vesicle-plasma membrane fusion in rat mast cell granules is followed by a variable, exponentially distributed, delay before bulk release. This delay reflects the time required for the expansion of the exocytotic fusion pore, lasting, on average, 231 ms in resting cytosolic calcium, [Ca
2+]
i (50 nM). In the presence of [Ca
2+]
i in the low micromollar range, the lag between fusion and release was reduced to 123 ms. The characteristics of the amperometric signals were unchanged by [Ca
2+]
i. These results show a novel site of regulation in the exocytotic process, the fusion pore, which may represent a different mechanism facilitating transmitter release.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>7737406</pmid><doi>10.1016/0014-5793(95)00319-5</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | FEBS letters, 1995-04, Vol.363 (3), p.221-225 |
| issn | 0014-5793 1873-3468 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Access via ScienceDirect (Elsevier); Alma/SFX Local Collection |
| subjects | Amperometry Animals Calcium - physiology Cell capacitance Cell Degranulation Cytosol - physiology EGTA ethylene glycol-bis(β-aminoethyl ether) N,N,N′,N′-tetraacetic Exocytosis HEPES Mast Cells - physiology Membrane Fusion Membrane Potentials Mice Mice, Mutant Strains N-[2-hydroxyethyl]piperazine-N′-[2-ethanesulfo nic acid), GTPγS, guanosine-5′-O-(3-thiotriphosphate Patch-Clamp Techniques Rats Serotonin - metabolism Transmitter release |
| title | Cytosolic calcium facilitates release of secretory products after exocytotic vesicle fusion |
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