Superinfection of a defective human immunodeficiency virus type 1 provirus-carrying T cell clone with vif or vpu mutants gives cytopathic virus particles by homologous recombination
The partially CD4-expressing T cell clone, Vpr-1, which carries a latent vpr-defective HIV-1 genome and expresses HIV-1 Nef protein only, was permissive to superinfection by HIV-1. Superinfection of Vpr-1 with vif- or vpu-defective mutants, which were noncytopathic, reactivated the vpr-defective vir...
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| Veröffentlicht in: | AIDS research and human retroviruses 1995, Vol.11 (1), p.45-53 |
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| container_title | AIDS research and human retroviruses |
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| creator | KISHI, M TOKUNAGA, K YONG-HUI ZHENG MIRZA KHALIL BAHMANI KAKINUMA, M NONOYAMA, M LAI, P. K IKUTA, K |
| description | The partially CD4-expressing T cell clone, Vpr-1, which carries a latent vpr-defective HIV-1 genome and expresses HIV-1 Nef protein only, was permissive to superinfection by HIV-1. Superinfection of Vpr-1 with vif- or vpu-defective mutants, which were noncytopathic, reactivated the vpr-defective virus and led to homologous recombination and cytopathogenesis. The data provide an experimental model for homologous recombination being an important mechanism whereby HIV-1 acquires genetic heterogeneity, and when occurring among defective virus in vivo bestows novel biological activities and virulence. |
| doi_str_mv | 10.1089/aid.1995.11.45 |
| format | Article |
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The data provide an experimental model for homologous recombination being an important mechanism whereby HIV-1 acquires genetic heterogeneity, and when occurring among defective virus in vivo bestows novel biological activities and virulence.</description><identifier>ISSN: 0889-2229</identifier><identifier>EISSN: 1931-8405</identifier><identifier>DOI: 10.1089/aid.1995.11.45</identifier><identifier>PMID: 7734196</identifier><identifier>CODEN: ARHRE7</identifier><language>eng</language><publisher>Larchmont, NY: Liebert</publisher><subject>AIDS/HIV ; Biological and medical sciences ; CD4-Positive T-Lymphocytes - metabolism ; CD4-Positive T-Lymphocytes - virology ; Clone Cells ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Viral ; Gene Products, nef - biosynthesis ; Gene Products, nef - genetics ; Gene Products, vif - biosynthesis ; Gene Products, vif - genetics ; Gene Products, vpr - biosynthesis ; Gene Products, vpr - genetics ; Genetics ; Genome, Viral ; HIV Infections - genetics ; HIV-1 - genetics ; HIV-1 - pathogenicity ; human immunodeficiency virus 1 ; Humans ; Microbiology ; Mutation ; nef Gene Products, Human Immunodeficiency Virus ; Recombination, Genetic ; Superinfection - genetics ; vif Gene Products, Human Immunodeficiency Virus ; Virology ; vpr Gene Products, Human Immunodeficiency Virus</subject><ispartof>AIDS research and human retroviruses, 1995, Vol.11 (1), p.45-53</ispartof><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c350t-a1e1da7d07b22cc73f7ff2a4b64fbad548a1d0fcb6cb8099183c4b4c9b5fa90b3</citedby><cites>FETCH-LOGICAL-c350t-a1e1da7d07b22cc73f7ff2a4b64fbad548a1d0fcb6cb8099183c4b4c9b5fa90b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3042,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3472477$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7734196$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>KISHI, M</creatorcontrib><creatorcontrib>TOKUNAGA, K</creatorcontrib><creatorcontrib>YONG-HUI ZHENG</creatorcontrib><creatorcontrib>MIRZA KHALIL BAHMANI</creatorcontrib><creatorcontrib>KAKINUMA, M</creatorcontrib><creatorcontrib>NONOYAMA, M</creatorcontrib><creatorcontrib>LAI, P. K</creatorcontrib><creatorcontrib>IKUTA, K</creatorcontrib><title>Superinfection of a defective human immunodeficiency virus type 1 provirus-carrying T cell clone with vif or vpu mutants gives cytopathic virus particles by homologous recombination</title><title>AIDS research and human retroviruses</title><addtitle>AIDS Res Hum Retroviruses</addtitle><description>The partially CD4-expressing T cell clone, Vpr-1, which carries a latent vpr-defective HIV-1 genome and expresses HIV-1 Nef protein only, was permissive to superinfection by HIV-1. Superinfection of Vpr-1 with vif- or vpu-defective mutants, which were noncytopathic, reactivated the vpr-defective virus and led to homologous recombination and cytopathogenesis. The data provide an experimental model for homologous recombination being an important mechanism whereby HIV-1 acquires genetic heterogeneity, and when occurring among defective virus in vivo bestows novel biological activities and virulence.</description><subject>AIDS/HIV</subject><subject>Biological and medical sciences</subject><subject>CD4-Positive T-Lymphocytes - metabolism</subject><subject>CD4-Positive T-Lymphocytes - virology</subject><subject>Clone Cells</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Viral</subject><subject>Gene Products, nef - biosynthesis</subject><subject>Gene Products, nef - genetics</subject><subject>Gene Products, vif - biosynthesis</subject><subject>Gene Products, vif - genetics</subject><subject>Gene Products, vpr - biosynthesis</subject><subject>Gene Products, vpr - genetics</subject><subject>Genetics</subject><subject>Genome, Viral</subject><subject>HIV Infections - genetics</subject><subject>HIV-1 - genetics</subject><subject>HIV-1 - pathogenicity</subject><subject>human immunodeficiency virus 1</subject><subject>Humans</subject><subject>Microbiology</subject><subject>Mutation</subject><subject>nef Gene Products, Human Immunodeficiency Virus</subject><subject>Recombination, Genetic</subject><subject>Superinfection - genetics</subject><subject>vif Gene Products, Human Immunodeficiency Virus</subject><subject>Virology</subject><subject>vpr Gene Products, Human Immunodeficiency Virus</subject><issn>0889-2229</issn><issn>1931-8405</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v1DAQxS0EKkvhyg3JB8Qti-3Y6_iIqvJHqsSBco7sib1rlNjBdhblg_H98LZRr5xGM-83TzN6CL2lZE9Jpz5qP-ypUmJP6Z6LZ2hHVUubjhPxHO1I16mGMaZeolc5_yKEKMbEFbqSsuVUHXbo749ltskHZ6H4GHB0WOPBPrRni0_LpAP207SEWKcevA2w4rNPS8ZlnS2meE7xoW9Ap7T6cMT3GOw4YhhjsPiPL6e64HBM-DwveFqKDiXjY_XPGNYSZ11OHjbTWafiYaySWfEpTnGMx1jnyUKcjA_6cuZr9MLpMds3W71GPz_f3t98be6-f_l28-mugVaQ0mhq6aDlQKRhDEC2TjrHNDcH7oweBO80HYgDcwDTEaVo1wI3HJQRTiti2mv04dG3_vh7sbn0k8-X33Sw9aheSiaoFN1_QXroWkZaWcH9Iwgp5pys6-fkJ53WnpL-EmhfA-0vgfaU9lzUhXeb82ImOzzhW4JVf7_pOoMeXdIBfH7CWi4Zr-w_-5-vdQ</recordid><startdate>1995</startdate><enddate>1995</enddate><creator>KISHI, M</creator><creator>TOKUNAGA, K</creator><creator>YONG-HUI ZHENG</creator><creator>MIRZA KHALIL BAHMANI</creator><creator>KAKINUMA, M</creator><creator>NONOYAMA, M</creator><creator>LAI, P. 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K ; IKUTA, K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c350t-a1e1da7d07b22cc73f7ff2a4b64fbad548a1d0fcb6cb8099183c4b4c9b5fa90b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>AIDS/HIV</topic><topic>Biological and medical sciences</topic><topic>CD4-Positive T-Lymphocytes - metabolism</topic><topic>CD4-Positive T-Lymphocytes - virology</topic><topic>Clone Cells</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Viral</topic><topic>Gene Products, nef - biosynthesis</topic><topic>Gene Products, nef - genetics</topic><topic>Gene Products, vif - biosynthesis</topic><topic>Gene Products, vif - genetics</topic><topic>Gene Products, vpr - biosynthesis</topic><topic>Gene Products, vpr - genetics</topic><topic>Genetics</topic><topic>Genome, Viral</topic><topic>HIV Infections - genetics</topic><topic>HIV-1 - genetics</topic><topic>HIV-1 - pathogenicity</topic><topic>human immunodeficiency virus 1</topic><topic>Humans</topic><topic>Microbiology</topic><topic>Mutation</topic><topic>nef Gene Products, Human Immunodeficiency Virus</topic><topic>Recombination, Genetic</topic><topic>Superinfection - genetics</topic><topic>vif Gene Products, Human Immunodeficiency Virus</topic><topic>Virology</topic><topic>vpr Gene Products, Human Immunodeficiency Virus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>KISHI, M</creatorcontrib><creatorcontrib>TOKUNAGA, K</creatorcontrib><creatorcontrib>YONG-HUI ZHENG</creatorcontrib><creatorcontrib>MIRZA KHALIL BAHMANI</creatorcontrib><creatorcontrib>KAKINUMA, M</creatorcontrib><creatorcontrib>NONOYAMA, M</creatorcontrib><creatorcontrib>LAI, P. 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K</au><au>IKUTA, K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Superinfection of a defective human immunodeficiency virus type 1 provirus-carrying T cell clone with vif or vpu mutants gives cytopathic virus particles by homologous recombination</atitle><jtitle>AIDS research and human retroviruses</jtitle><addtitle>AIDS Res Hum Retroviruses</addtitle><date>1995</date><risdate>1995</risdate><volume>11</volume><issue>1</issue><spage>45</spage><epage>53</epage><pages>45-53</pages><issn>0889-2229</issn><eissn>1931-8405</eissn><coden>ARHRE7</coden><abstract>The partially CD4-expressing T cell clone, Vpr-1, which carries a latent vpr-defective HIV-1 genome and expresses HIV-1 Nef protein only, was permissive to superinfection by HIV-1. Superinfection of Vpr-1 with vif- or vpu-defective mutants, which were noncytopathic, reactivated the vpr-defective virus and led to homologous recombination and cytopathogenesis. 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| fulltext | fulltext |
| identifier | ISSN: 0889-2229 |
| ispartof | AIDS research and human retroviruses, 1995, Vol.11 (1), p.45-53 |
| issn | 0889-2229 1931-8405 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77251758 |
| source | Mary Ann Liebert Online Subscription; MEDLINE |
| subjects | AIDS/HIV Biological and medical sciences CD4-Positive T-Lymphocytes - metabolism CD4-Positive T-Lymphocytes - virology Clone Cells Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Viral Gene Products, nef - biosynthesis Gene Products, nef - genetics Gene Products, vif - biosynthesis Gene Products, vif - genetics Gene Products, vpr - biosynthesis Gene Products, vpr - genetics Genetics Genome, Viral HIV Infections - genetics HIV-1 - genetics HIV-1 - pathogenicity human immunodeficiency virus 1 Humans Microbiology Mutation nef Gene Products, Human Immunodeficiency Virus Recombination, Genetic Superinfection - genetics vif Gene Products, Human Immunodeficiency Virus Virology vpr Gene Products, Human Immunodeficiency Virus |
| title | Superinfection of a defective human immunodeficiency virus type 1 provirus-carrying T cell clone with vif or vpu mutants gives cytopathic virus particles by homologous recombination |
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