Unusually high-level expression of a foreign gene (hepatitis B virus core antigen) in Saccharomyces cerevisiae

As a model system for the study of factors affecting gene expression, hepatitis B virus core antigen (HBcAg) has been expressed in the yeast Saccharomyces cerevisiae. The singulariy high levels of expression achieved are approx. 40% of the soluble yeast protein. The HBcAg polypeptides are present as...

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Veröffentlicht in:	Gene 1986, Vol.46 (1), p.135-141
Hauptverfasser:	Kniskem, Peter J., Hagopian, Arpi, Montgomery, Donna L., Burke, Pamela, Dunn, Nancy R., Hofmann, Kathryn J., Miller, William J., Ellis, Ronald W.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology effect of nontranslated DNA Fundamental and applied biological sciences. Psychology gene expression Genes Genes, Viral Genetic engineering Genetic technics glyceraldehyde phosphate dehydrogenase promoter hepatitis B core antigen Hepatitis B Core Antigens - genetics Hepatitis B virus - genetics Hepatitis B virus - immunology Humans Methods. Procedures. Technologies Molecular Weight Plasmids promoters Recombinant DNA RNA, Messenger - analysis Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Transcription, Genetic Vectors (cloning, transfer, expression). Insertion sequences and transposons
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