Cytoplasmic and Nucleic Calcium Oscillations in Immature Mouse Oocytes: Evidence of Wave Polarization by Confocal Imaging

Using confocal laser scanning microscopy and time-lapse reconstruction, we monitored [Ca 2+] i in living mouse oocytes loaded with the calcium-sensitive fluorescent dyes fluo-3/AM or NuCa Green (specific for nuclear calcium) at the time of meiosis reinitiation. Our data confirm that spontaneous Ca 2...

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Veröffentlicht in:	Experimental cell research 1995-05, Vol.218 (1), p.166-173
Hauptverfasser:	Lefèvre, Brigitte, Pesty, Arlette, Testart, Jacques
Format:	Artikel
Sprache:	eng
Schlagworte:	Aniline Compounds Animals Bucladesine - pharmacology Calcium - metabolism Calcium-Transporting ATPases - antagonists & inhibitors Cell Nucleus - metabolism Cells, Cultured Chromatin - drug effects Chromatin - metabolism Chromatin - ultrastructure Cytoplasm - metabolism Dextrans Female Fluorescent Dyes Kinetics Meiosis - physiology Mice Microinjections Microscopy, Confocal - methods Oocytes - cytology Oocytes - drug effects Oocytes - metabolism Terpenes - pharmacology Thapsigargin Time Factors Xanthenes
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