Growth behaviour and glucoamylase production by Aspergillus niger N402 and a glucoamylase overproducing transformant in recycling culture without a nitrogen source
When wild-type Aspergillus niger N402 and a glucoamylase-overproducing transformant were grown in recycling culture without a nitrogen source, hyphal tip extension and glucoamylase production still occurred, but overproduction of glucoamylase by the transformant strain stopped. The mycelium retained...
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| Veröffentlicht in: | Applied microbiology and biotechnology 1995-04, Vol.43 (1), p.109-116 |
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| creator | Schrickx, J.M Stouthamer, A.H Verseveld, H.W. van |
| description | When wild-type Aspergillus niger N402 and a glucoamylase-overproducing transformant were grown in recycling culture without a nitrogen source, hyphal tip extension and glucoamylase production still occurred, but overproduction of glucoamylase by the transformant strain stopped. The mycelium retained a low metabolic activity. Light micrographs of mycelial samples showed that some hyphae were broken at their tip and partially empty, while after continuing recycling fermentation for more than 500 h many small and empty pieces of broken mycelium could be found. A model has been developed to calculate the mycelial growth and death rates. The mycelial death rate just exceeded the mycelial growth rate and as a consequence the amount of biomass in the fermentor vessel slightly decreased. It is concluded that the cytoplasmic contents of broken mycelial threads were released into the medium and acted as a nitrogen source for the growing parts of the mycelium. |
| doi_str_mv | 10.1007/BF00170631 |
| format | Article |
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The mycelium retained a low metabolic activity. Light micrographs of mycelial samples showed that some hyphae were broken at their tip and partially empty, while after continuing recycling fermentation for more than 500 h many small and empty pieces of broken mycelium could be found. A model has been developed to calculate the mycelial growth and death rates. The mycelial death rate just exceeded the mycelial growth rate and as a consequence the amount of biomass in the fermentor vessel slightly decreased. 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The mycelium retained a low metabolic activity. Light micrographs of mycelial samples showed that some hyphae were broken at their tip and partially empty, while after continuing recycling fermentation for more than 500 h many small and empty pieces of broken mycelium could be found. A model has been developed to calculate the mycelial growth and death rates. The mycelial death rate just exceeded the mycelial growth rate and as a consequence the amount of biomass in the fermentor vessel slightly decreased. It is concluded that the cytoplasmic contents of broken mycelial threads were released into the medium and acted as a nitrogen source for the growing parts of the mycelium.</description><subject>Aspergillus niger</subject><subject>Aspergillus niger - cytology</subject><subject>Aspergillus niger - growth & development</subject><subject>Aspergillus niger - metabolism</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>cell death rate</subject><subject>fermentation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genetic transformation</subject><subject>glucan 1,4-alpha-glucosidase</subject><subject>Glucan 1,4-alpha-Glucosidase - biosynthesis</subject><subject>growth rate</subject><subject>hyphae</subject><subject>mass transfer</subject><subject>Methods. Procedures. Technologies</subject><subject>Microbial engineering. 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Fermentation and microbial culture technology</topic><topic>mycelium</topic><topic>Nitrogen - metabolism</topic><topic>simulation models</topic><topic>Transformation, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schrickx, J.M</creatorcontrib><creatorcontrib>Stouthamer, A.H</creatorcontrib><creatorcontrib>Verseveld, H.W. van</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Applied microbiology and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schrickx, J.M</au><au>Stouthamer, A.H</au><au>Verseveld, H.W. van</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Growth behaviour and glucoamylase production by Aspergillus niger N402 and a glucoamylase overproducing transformant in recycling culture without a nitrogen source</atitle><jtitle>Applied microbiology and biotechnology</jtitle><addtitle>Appl Microbiol Biotechnol</addtitle><date>1995-04-01</date><risdate>1995</risdate><volume>43</volume><issue>1</issue><spage>109</spage><epage>116</epage><pages>109-116</pages><issn>0175-7598</issn><eissn>1432-0614</eissn><coden>AMBIDG</coden><abstract>When wild-type Aspergillus niger N402 and a glucoamylase-overproducing transformant were grown in recycling culture without a nitrogen source, hyphal tip extension and glucoamylase production still occurred, but overproduction of glucoamylase by the transformant strain stopped. The mycelium retained a low metabolic activity. Light micrographs of mycelial samples showed that some hyphae were broken at their tip and partially empty, while after continuing recycling fermentation for more than 500 h many small and empty pieces of broken mycelium could be found. A model has been developed to calculate the mycelial growth and death rates. The mycelial death rate just exceeded the mycelial growth rate and as a consequence the amount of biomass in the fermentor vessel slightly decreased. It is concluded that the cytoplasmic contents of broken mycelial threads were released into the medium and acted as a nitrogen source for the growing parts of the mycelium.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>7766124</pmid><doi>10.1007/BF00170631</doi><tpages>8</tpages></addata></record> |
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| ispartof | Applied microbiology and biotechnology, 1995-04, Vol.43 (1), p.109-116 |
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| subjects | Aspergillus niger Aspergillus niger - cytology Aspergillus niger - growth & development Aspergillus niger - metabolism Biological and medical sciences Biotechnology cell death rate fermentation Fundamental and applied biological sciences. Psychology genetic transformation glucan 1,4-alpha-glucosidase Glucan 1,4-alpha-Glucosidase - biosynthesis growth rate hyphae mass transfer Methods. Procedures. Technologies Microbial engineering. Fermentation and microbial culture technology mycelium Nitrogen - metabolism simulation models Transformation, Genetic |
| title | Growth behaviour and glucoamylase production by Aspergillus niger N402 and a glucoamylase overproducing transformant in recycling culture without a nitrogen source |
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