A solid-phase assay for the phosphorylation of proteins blotted on nitrocellulose membrane filters

A new procedure for the phosphorylation and assay of phosphoproteins is described. Proteins are solubilized from tissue samples, separated by polyacrylamide gel electrophoresis, transferred onto nitrocellulose membrane filters, and the blotted polypeptides are phosphorylated with the catalytic subun...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Anal. Biochem.; (United States) 1986-10, Vol.158 (1), p.130-137
Hauptverfasser:	Valtorta, Flavia, Schiebler, Werner, Jahn, Reinhard, Ceccarelli, Bruno, Greengard, Paul
Format:	Artikel
Sprache:	eng
Schlagworte:	550201 - Biochemistry- Tracer Techniques 550601 - Medicine- Unsealed Radionuclides in Diagnostics AMP Analytical, structural and metabolic biochemistry Animals Applied sciences BASIC BIOLOGICAL SCIENCES BETA DECAY RADIOISOTOPES BETA-MINUS DECAY RADIOISOTOPES Biological and medical sciences Cattle CHEMICAL REACTIONS Collodion DAYS LIVING RADIOISOTOPES ELECTROPHORESIS ENZYMES Exact sciences and technology Fundamental and applied biological sciences. Psychology Holoproteins ISOTOPES LIGHT NUCLEI Male MEMBRANES Nerve Tissue Proteins - analysis neurochemistry nitrocellulose NUCLEI NUCLEOTIDES ODD-ODD NUCLEI ORGANIC COMPOUNDS Other proteins Other techniques and industries Phosphoproteins - analysis PHOSPHORUS 32 PHOSPHORUS ISOTOPES PHOSPHORUS-GROUP TRANSFERASES PHOSPHORYLATION PHOSPHOTRANSFERASES protein blotting protein kinase Protein Kinases protein phosphorylation PROTEINS RADIOASSAY RADIOISOTOPES RADIOLOGY AND NUCLEAR MEDICINE Rats solid phase methods synapsin I Synapsins TRANSFERASES
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!