Biliary Nonmucin Glycoproteins in Patients with and without Gallstones
Total protein, mucin, and specific nonmucin glycoproteins are proposed pronucleating agents in cholesterol gallstone pathogenesis. However, characterization of specific nonmucin glycoproteins in patients with and without gallstones is unknown. Furthermore, nonmucin glycoproteins may be qualitatively...
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| Veröffentlicht in: | The Journal of surgical research 1995-04, Vol.58 (4), p.386-390 |
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| creator | Lipsett, Pamela A. Fox-Talbot, M.Karen Falconer, Sarah D. Tam, Michael L. Magnuson, Thomas A. Lillemoe, Keith D. Pitt, Henry A. |
| description | Total protein, mucin, and specific nonmucin glycoproteins are proposed pronucleating agents in cholesterol gallstone pathogenesis. However, characterization of specific nonmucin glycoproteins in patients with and without gallstones is unknown. Furthermore, nonmucin glycoproteins may be qualitatively different in patients with and without gallstones. Total protein and total and specific nonmucin glycoproteins were studied in gallbladder bile of 43 patients with cholesterol gallstones and 13 patients without gallstones. Patients with cholesterol gallstones had higher concentrations of both total protein and nonmucin glycoproteins than that observed in control patients (P < 0.05). SDS gel electrophoresis of nonmucin glycoproteins demonstrated an 84-kDa protein that was present significantly more often in patients with cholesterol gallstones (87% vs 8%, P < 0.05). Proposed 130- and 42-kDa pronucleating and 120-kDa anti-nucleating nonmucin glycoproteins were present in similar percentages in gallstone and control bile. Moreover, gallbladder bile of patients with the 84-kDa protein nucleated 50% faster than model bile and >100% faster than that of patients without this protein (P < 0.05). The currently described gallbladder pronucleating and anti-nucleating proteins are found with equal frequency in cholesterol gallstone and control patients. However, an 84-kDa protein is found more commonly in gallstone patients and was associated with a shortened crystal observation time. Thus, this glycoprotein may be important in cholesterol gallstone pathogenesis. |
| doi_str_mv | 10.1006/jsre.1995.1059 |
| format | Article |
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However, characterization of specific nonmucin glycoproteins in patients with and without gallstones is unknown. Furthermore, nonmucin glycoproteins may be qualitatively different in patients with and without gallstones. Total protein and total and specific nonmucin glycoproteins were studied in gallbladder bile of 43 patients with cholesterol gallstones and 13 patients without gallstones. Patients with cholesterol gallstones had higher concentrations of both total protein and nonmucin glycoproteins than that observed in control patients (P < 0.05). SDS gel electrophoresis of nonmucin glycoproteins demonstrated an 84-kDa protein that was present significantly more often in patients with cholesterol gallstones (87% vs 8%, P < 0.05). Proposed 130- and 42-kDa pronucleating and 120-kDa anti-nucleating nonmucin glycoproteins were present in similar percentages in gallstone and control bile. Moreover, gallbladder bile of patients with the 84-kDa protein nucleated 50% faster than model bile and >100% faster than that of patients without this protein (P < 0.05). The currently described gallbladder pronucleating and anti-nucleating proteins are found with equal frequency in cholesterol gallstone and control patients. However, an 84-kDa protein is found more commonly in gallstone patients and was associated with a shortened crystal observation time. Thus, this glycoprotein may be important in cholesterol gallstone pathogenesis.</description><identifier>ISSN: 0022-4804</identifier><identifier>EISSN: 1095-8673</identifier><identifier>DOI: 10.1006/jsre.1995.1059</identifier><identifier>PMID: 7723316</identifier><identifier>CODEN: JSGRA2</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Bile - metabolism ; Biological and medical sciences ; Cholelithiasis - metabolism ; Cholesterol - metabolism ; Concanavalin A ; Crystallization ; Electrophoresis, Polyacrylamide Gel ; Female ; Gallbladder - metabolism ; Gastroenterology. Liver. Pancreas. Abdomen ; Glycoproteins - metabolism ; Humans ; Liver. Biliary tract. Portal circulation. Exocrine pancreas ; Male ; Medical sciences ; Middle Aged ; Other diseases. Semiology ; Reference Values</subject><ispartof>The Journal of surgical research, 1995-04, Vol.58 (4), p.386-390</ispartof><rights>1995 Academic Press</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c408t-fad717934d232e3ac0506ecdd29770a37d05656a340989b4f5b2d7835d56538a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0022480485710591$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3504761$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7723316$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lipsett, Pamela A.</creatorcontrib><creatorcontrib>Fox-Talbot, M.Karen</creatorcontrib><creatorcontrib>Falconer, Sarah D.</creatorcontrib><creatorcontrib>Tam, Michael L.</creatorcontrib><creatorcontrib>Magnuson, Thomas A.</creatorcontrib><creatorcontrib>Lillemoe, Keith D.</creatorcontrib><creatorcontrib>Pitt, Henry A.</creatorcontrib><title>Biliary Nonmucin Glycoproteins in Patients with and without Gallstones</title><title>The Journal of surgical research</title><addtitle>J Surg Res</addtitle><description>Total protein, mucin, and specific nonmucin glycoproteins are proposed pronucleating agents in cholesterol gallstone pathogenesis. However, characterization of specific nonmucin glycoproteins in patients with and without gallstones is unknown. Furthermore, nonmucin glycoproteins may be qualitatively different in patients with and without gallstones. Total protein and total and specific nonmucin glycoproteins were studied in gallbladder bile of 43 patients with cholesterol gallstones and 13 patients without gallstones. Patients with cholesterol gallstones had higher concentrations of both total protein and nonmucin glycoproteins than that observed in control patients (P < 0.05). SDS gel electrophoresis of nonmucin glycoproteins demonstrated an 84-kDa protein that was present significantly more often in patients with cholesterol gallstones (87% vs 8%, P < 0.05). Proposed 130- and 42-kDa pronucleating and 120-kDa anti-nucleating nonmucin glycoproteins were present in similar percentages in gallstone and control bile. Moreover, gallbladder bile of patients with the 84-kDa protein nucleated 50% faster than model bile and >100% faster than that of patients without this protein (P < 0.05). The currently described gallbladder pronucleating and anti-nucleating proteins are found with equal frequency in cholesterol gallstone and control patients. However, an 84-kDa protein is found more commonly in gallstone patients and was associated with a shortened crystal observation time. Thus, this glycoprotein may be important in cholesterol gallstone pathogenesis.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Bile - metabolism</subject><subject>Biological and medical sciences</subject><subject>Cholelithiasis - metabolism</subject><subject>Cholesterol - metabolism</subject><subject>Concanavalin A</subject><subject>Crystallization</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Female</subject><subject>Gallbladder - metabolism</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>Glycoproteins - metabolism</subject><subject>Humans</subject><subject>Liver. Biliary tract. Portal circulation. Exocrine pancreas</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Other diseases. Semiology</subject><subject>Reference Values</subject><issn>0022-4804</issn><issn>1095-8673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM9PwyAUgInRzDm9ejPpwXjrhFKgHHVx02RRD3omDGhkaWEWqtl_L3WNN0-8Hx8v730AXCI4RxDS223ozBxxTlJK-BGYIshJXlGGj8EUwqLIywqWp-AshC1MOWd4AiaMFRgjOgXLe9tY2e2zZ-_aXlmXrZq98rvOR2NdyFLhVUZrXAzZt40fmXT6N_B9zFayaUL0zoRzcFLLJpiL8Z2B9-XD2-IxX7-snhZ361yVsIp5LTVDjONSF7gwWCpIIDVK67QWgxIzDQklVOIS8opvyppsCs0qTHQq40riGbg5zE0LfvYmRNHaoEzTSGd8H8RwFyowTeD8AKrOh6SoFrvOtulQgaAYxIlBnBjEiUFc-nA1Tu43rdF_-Ggq9a_HvgxKNnUnnbLhD8MEloyihFUHzCQLX9Z0IqhkTxltO6Oi0N7-t8EPPnaI-w</recordid><startdate>19950401</startdate><enddate>19950401</enddate><creator>Lipsett, Pamela A.</creator><creator>Fox-Talbot, M.Karen</creator><creator>Falconer, Sarah D.</creator><creator>Tam, Michael L.</creator><creator>Magnuson, Thomas A.</creator><creator>Lillemoe, Keith D.</creator><creator>Pitt, Henry A.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19950401</creationdate><title>Biliary Nonmucin Glycoproteins in Patients with and without Gallstones</title><author>Lipsett, Pamela A. ; Fox-Talbot, M.Karen ; Falconer, Sarah D. ; Tam, Michael L. ; Magnuson, Thomas A. ; Lillemoe, Keith D. ; Pitt, Henry A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-fad717934d232e3ac0506ecdd29770a37d05656a340989b4f5b2d7835d56538a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Bile - metabolism</topic><topic>Biological and medical sciences</topic><topic>Cholelithiasis - metabolism</topic><topic>Cholesterol - metabolism</topic><topic>Concanavalin A</topic><topic>Crystallization</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Female</topic><topic>Gallbladder - metabolism</topic><topic>Gastroenterology. Liver. Pancreas. Abdomen</topic><topic>Glycoproteins - metabolism</topic><topic>Humans</topic><topic>Liver. Biliary tract. Portal circulation. Exocrine pancreas</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Other diseases. Semiology</topic><topic>Reference Values</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lipsett, Pamela A.</creatorcontrib><creatorcontrib>Fox-Talbot, M.Karen</creatorcontrib><creatorcontrib>Falconer, Sarah D.</creatorcontrib><creatorcontrib>Tam, Michael L.</creatorcontrib><creatorcontrib>Magnuson, Thomas A.</creatorcontrib><creatorcontrib>Lillemoe, Keith D.</creatorcontrib><creatorcontrib>Pitt, Henry A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of surgical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lipsett, Pamela A.</au><au>Fox-Talbot, M.Karen</au><au>Falconer, Sarah D.</au><au>Tam, Michael L.</au><au>Magnuson, Thomas A.</au><au>Lillemoe, Keith D.</au><au>Pitt, Henry A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biliary Nonmucin Glycoproteins in Patients with and without Gallstones</atitle><jtitle>The Journal of surgical research</jtitle><addtitle>J Surg Res</addtitle><date>1995-04-01</date><risdate>1995</risdate><volume>58</volume><issue>4</issue><spage>386</spage><epage>390</epage><pages>386-390</pages><issn>0022-4804</issn><eissn>1095-8673</eissn><coden>JSGRA2</coden><abstract>Total protein, mucin, and specific nonmucin glycoproteins are proposed pronucleating agents in cholesterol gallstone pathogenesis. However, characterization of specific nonmucin glycoproteins in patients with and without gallstones is unknown. Furthermore, nonmucin glycoproteins may be qualitatively different in patients with and without gallstones. Total protein and total and specific nonmucin glycoproteins were studied in gallbladder bile of 43 patients with cholesterol gallstones and 13 patients without gallstones. Patients with cholesterol gallstones had higher concentrations of both total protein and nonmucin glycoproteins than that observed in control patients (P < 0.05). SDS gel electrophoresis of nonmucin glycoproteins demonstrated an 84-kDa protein that was present significantly more often in patients with cholesterol gallstones (87% vs 8%, P < 0.05). Proposed 130- and 42-kDa pronucleating and 120-kDa anti-nucleating nonmucin glycoproteins were present in similar percentages in gallstone and control bile. Moreover, gallbladder bile of patients with the 84-kDa protein nucleated 50% faster than model bile and >100% faster than that of patients without this protein (P < 0.05). The currently described gallbladder pronucleating and anti-nucleating proteins are found with equal frequency in cholesterol gallstone and control patients. However, an 84-kDa protein is found more commonly in gallstone patients and was associated with a shortened crystal observation time. Thus, this glycoprotein may be important in cholesterol gallstone pathogenesis.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>7723316</pmid><doi>10.1006/jsre.1995.1059</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Aged Aged, 80 and over Bile - metabolism Biological and medical sciences Cholelithiasis - metabolism Cholesterol - metabolism Concanavalin A Crystallization Electrophoresis, Polyacrylamide Gel Female Gallbladder - metabolism Gastroenterology. Liver. Pancreas. Abdomen Glycoproteins - metabolism Humans Liver. Biliary tract. Portal circulation. Exocrine pancreas Male Medical sciences Middle Aged Other diseases. Semiology Reference Values |
| title | Biliary Nonmucin Glycoproteins in Patients with and without Gallstones |
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