The Role of Calcium in Stimulation of Activated T Lymphocytes with Interleukin 2
In a study of the role of Ca in the stimulation of activated T lymphocytes with interleukin 2 (IL‐2) it was found that IL‐2‐induced proliferation can occur independently of extracellular calcium. Further, there was no correlation between triggering of DNA synthesis and an increase in free cytoplasmi...
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| Veröffentlicht in: | Scandinavian journal of immunology 1986-12, Vol.24 (6), p.689-697 |
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| container_title | Scandinavian journal of immunology |
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| creator | LARSEN, C. S. KNUDSEN, T. E. JOHNSEN, H. E. |
| description | In a study of the role of Ca in the stimulation of activated T lymphocytes with interleukin 2 (IL‐2) it was found that IL‐2‐induced proliferation can occur independently of extracellular calcium. Further, there was no correlation between triggering of DNA synthesis and an increase in free cytoplasmic calcium. However. IL‐2 induced an increased uptake of 45Ca from the extracellular medium. Since there is no increase in free cytoplasmic calcium, it must be assumed that this is caused by an increase in membrane‐associated calcium. Further, the calcium channel‐blocking agent, verapamil, and TMB‐8, a putative inhibitor of mobilization of calcium from intracellular pools, both exerted a dose‐dependent inhibition of IL‐2‐induced DNA synthesis in activated T lymphocytes. We conclude that calcium is not a second messenger in activated T lymphocytes stimulated by IL‐2, but our results indicate that calcium may play a role at membrane level. |
| doi_str_mv | 10.1111/j.1365-3083.1986.tb02188.x |
| format | Article |
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We conclude that calcium is not a second messenger in activated T lymphocytes stimulated by IL‐2, but our results indicate that calcium may play a role at membrane level.</description><identifier>ISSN: 0300-9475</identifier><identifier>EISSN: 1365-3083</identifier><identifier>DOI: 10.1111/j.1365-3083.1986.tb02188.x</identifier><identifier>PMID: 3099381</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Calcimycin - pharmacology ; Calcium - analysis ; Calcium - physiology ; Cells, Cultured ; Egtazic Acid - pharmacology ; Gallic Acid - analogs & derivatives ; Gallic Acid - pharmacology ; Humans ; Interleukin-2 ; Lymphocyte Activation ; Phytohemagglutinins - pharmacology ; T-Lymphocytes - immunology ; Verapamil - pharmacology</subject><ispartof>Scandinavian journal of immunology, 1986-12, Vol.24 (6), p.689-697</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2849-64781cceee73515f9394aad6404774b719498fc094bc6d926342af8426c3cdba3</citedby><cites>FETCH-LOGICAL-c2849-64781cceee73515f9394aad6404774b719498fc094bc6d926342af8426c3cdba3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-3083.1986.tb02188.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-3083.1986.tb02188.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27922,27923,45572,45573</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3099381$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>LARSEN, C. S.</creatorcontrib><creatorcontrib>KNUDSEN, T. E.</creatorcontrib><creatorcontrib>JOHNSEN, H. E.</creatorcontrib><title>The Role of Calcium in Stimulation of Activated T Lymphocytes with Interleukin 2</title><title>Scandinavian journal of immunology</title><addtitle>Scand J Immunol</addtitle><description>In a study of the role of Ca in the stimulation of activated T lymphocytes with interleukin 2 (IL‐2) it was found that IL‐2‐induced proliferation can occur independently of extracellular calcium. Further, there was no correlation between triggering of DNA synthesis and an increase in free cytoplasmic calcium. However. IL‐2 induced an increased uptake of 45Ca from the extracellular medium. Since there is no increase in free cytoplasmic calcium, it must be assumed that this is caused by an increase in membrane‐associated calcium. Further, the calcium channel‐blocking agent, verapamil, and TMB‐8, a putative inhibitor of mobilization of calcium from intracellular pools, both exerted a dose‐dependent inhibition of IL‐2‐induced DNA synthesis in activated T lymphocytes. We conclude that calcium is not a second messenger in activated T lymphocytes stimulated by IL‐2, but our results indicate that calcium may play a role at membrane level.</description><subject>Calcimycin - pharmacology</subject><subject>Calcium - analysis</subject><subject>Calcium - physiology</subject><subject>Cells, Cultured</subject><subject>Egtazic Acid - pharmacology</subject><subject>Gallic Acid - analogs & derivatives</subject><subject>Gallic Acid - pharmacology</subject><subject>Humans</subject><subject>Interleukin-2</subject><subject>Lymphocyte Activation</subject><subject>Phytohemagglutinins - pharmacology</subject><subject>T-Lymphocytes - immunology</subject><subject>Verapamil - pharmacology</subject><issn>0300-9475</issn><issn>1365-3083</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkF1LwzAUhoMoc05_ghC88K41X20SLwQZfkwGipvXIU1Tltmus0nd9u9t2di95-ZcPOd9DzwA3GAU427uljGmaRJRJGiMpUjjkCGChYi3J2B4RKdgiChCkWQ8OQcX3i8RwpRwOgADiqSkAg_Bx3xh4WddWlgXcKxL49oKuhWcBVe1pQ6uXvXk0QT3q4PN4RxOd9V6UZtdsB5uXFjAySrYprTtd5cjl-Cs0KW3V4c9Al_PT_PxazR9f5mMH6eRIYLJKGVcYGOstZwmOCkklUzrPGWIcc4yjiWTojBIssykuSQpZUQXgpHUUJNnmo7A7b533dQ_rfVBVc4bW5Z6ZevWK84JSbqW7vB-f2ia2vvGFmrduEo3O4WR6nWqpeqdqd6Z6nWqg0617cLXhy9tVtn8GD346_jDnm9caXf_aFazt0kqJP0DO8eELw</recordid><startdate>198612</startdate><enddate>198612</enddate><creator>LARSEN, C. S.</creator><creator>KNUDSEN, T. E.</creator><creator>JOHNSEN, H. E.</creator><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198612</creationdate><title>The Role of Calcium in Stimulation of Activated T Lymphocytes with Interleukin 2</title><author>LARSEN, C. S. ; KNUDSEN, T. E. ; JOHNSEN, H. E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2849-64781cceee73515f9394aad6404774b719498fc094bc6d926342af8426c3cdba3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Calcimycin - pharmacology</topic><topic>Calcium - analysis</topic><topic>Calcium - physiology</topic><topic>Cells, Cultured</topic><topic>Egtazic Acid - pharmacology</topic><topic>Gallic Acid - analogs & derivatives</topic><topic>Gallic Acid - pharmacology</topic><topic>Humans</topic><topic>Interleukin-2</topic><topic>Lymphocyte Activation</topic><topic>Phytohemagglutinins - pharmacology</topic><topic>T-Lymphocytes - immunology</topic><topic>Verapamil - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LARSEN, C. S.</creatorcontrib><creatorcontrib>KNUDSEN, T. E.</creatorcontrib><creatorcontrib>JOHNSEN, H. E.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Scandinavian journal of immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>LARSEN, C. S.</au><au>KNUDSEN, T. E.</au><au>JOHNSEN, H. E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Role of Calcium in Stimulation of Activated T Lymphocytes with Interleukin 2</atitle><jtitle>Scandinavian journal of immunology</jtitle><addtitle>Scand J Immunol</addtitle><date>1986-12</date><risdate>1986</risdate><volume>24</volume><issue>6</issue><spage>689</spage><epage>697</epage><pages>689-697</pages><issn>0300-9475</issn><eissn>1365-3083</eissn><abstract>In a study of the role of Ca in the stimulation of activated T lymphocytes with interleukin 2 (IL‐2) it was found that IL‐2‐induced proliferation can occur independently of extracellular calcium. Further, there was no correlation between triggering of DNA synthesis and an increase in free cytoplasmic calcium. However. IL‐2 induced an increased uptake of 45Ca from the extracellular medium. Since there is no increase in free cytoplasmic calcium, it must be assumed that this is caused by an increase in membrane‐associated calcium. Further, the calcium channel‐blocking agent, verapamil, and TMB‐8, a putative inhibitor of mobilization of calcium from intracellular pools, both exerted a dose‐dependent inhibition of IL‐2‐induced DNA synthesis in activated T lymphocytes. We conclude that calcium is not a second messenger in activated T lymphocytes stimulated by IL‐2, but our results indicate that calcium may play a role at membrane level.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>3099381</pmid><doi>10.1111/j.1365-3083.1986.tb02188.x</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0300-9475 |
| ispartof | Scandinavian journal of immunology, 1986-12, Vol.24 (6), p.689-697 |
| issn | 0300-9475 1365-3083 |
| language | eng |
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| source | MEDLINE; Wiley Online Library All Journals |
| subjects | Calcimycin - pharmacology Calcium - analysis Calcium - physiology Cells, Cultured Egtazic Acid - pharmacology Gallic Acid - analogs & derivatives Gallic Acid - pharmacology Humans Interleukin-2 Lymphocyte Activation Phytohemagglutinins - pharmacology T-Lymphocytes - immunology Verapamil - pharmacology |
| title | The Role of Calcium in Stimulation of Activated T Lymphocytes with Interleukin 2 |
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