Calcium-transport function of the chick embryonic chorioallantoic membrane. I. In vivo and in vitro characterization
During chick embryonic development, the chorioallantoic membrane (CAM) is responsible for the mobilization of shell calcium into the embryonic circulation. The calcium-transport function of the CAM was studied here by measuring CAM calcium uptake in vivo and in vitro. The in vivo technique involved...
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| Veröffentlicht in: | Journal of cell science 1986-06, Vol.82 (1), p.73-84 |
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| container_end_page | 84 |
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| container_start_page | 73 |
| container_title | Journal of cell science |
| container_volume | 82 |
| creator | Tuan, R.S Carson, M.J Jozefiak, J.A Knowles, K.A Shotwell, B.A |
| description | During chick embryonic development, the chorioallantoic membrane (CAM) is responsible for the mobilization of shell calcium into the embryonic circulation. The calcium-transport function of the CAM was studied here by measuring CAM calcium uptake in vivo and in vitro. The in vivo technique involved the use of an uptake chamber constructed on top of the CAM in situ. The in vitro methods included two systems: CAM tissue disks and cell-free microsomal membranes isolated from the CAM. Analyses using these three assays show that calcium uptake by the CAM exhibited characteristics indicative of active transport, such as temperature dependence, saturability, energetic requirement and ion specificity. The data also show that calcium-uptake activities of the CAM increase as a function of embryonic age in a manner coincident with the increased accumulation of calcium by the developing embryo in ovo. |
| doi_str_mv | 10.1242/jcs.82.1.73 |
| format | Article |
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Analyses using these three assays show that calcium uptake by the CAM exhibited characteristics indicative of active transport, such as temperature dependence, saturability, energetic requirement and ion specificity. The data also show that calcium-uptake activities of the CAM increase as a function of embryonic age in a manner coincident with the increased accumulation of calcium by the developing embryo in ovo.</description><identifier>ISSN: 0021-9533</identifier><identifier>EISSN: 1477-9137</identifier><identifier>DOI: 10.1242/jcs.82.1.73</identifier><identifier>PMID: 3793786</identifier><identifier>CODEN: JNCSAI</identifier><language>eng</language><publisher>Cambridge: Company of Biologists</publisher><subject>Animals ; Biological and medical sciences ; Biological Transport, Active ; calcium ; Calcium - metabolism ; characterization ; Chick Embryo ; chicks ; chorioallantoic membrane ; embryo (animal) ; embryogenesis ; Embryology: invertebrates and vertebrates. Teratology ; Embryonic and Fetal Development ; Extraembryonic Membranes - metabolism ; Extraembryonic Membranes - ultrastructure ; Fundamental and applied biological sciences. Psychology ; in vitro ; in vivo studies ; Microscopy, Electron ; Microsomes - ultrastructure ; nutrient transport ; Organogenesis. Fetal development ; Organogenesis. Physiological fonctions</subject><ispartof>Journal of cell science, 1986-06, Vol.82 (1), p.73-84</ispartof><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c285t-186c242323c4b224d74ed3932cdec5932b600275fcf873436b89ea003a04e53a3</citedby><cites>FETCH-LOGICAL-c285t-186c242323c4b224d74ed3932cdec5932b600275fcf873436b89ea003a04e53a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3678,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7983152$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3793786$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tuan, R.S</creatorcontrib><creatorcontrib>Carson, M.J</creatorcontrib><creatorcontrib>Jozefiak, J.A</creatorcontrib><creatorcontrib>Knowles, K.A</creatorcontrib><creatorcontrib>Shotwell, B.A</creatorcontrib><title>Calcium-transport function of the chick embryonic chorioallantoic membrane. I. In vivo and in vitro characterization</title><title>Journal of cell science</title><addtitle>J Cell Sci</addtitle><description>During chick embryonic development, the chorioallantoic membrane (CAM) is responsible for the mobilization of shell calcium into the embryonic circulation. The calcium-transport function of the CAM was studied here by measuring CAM calcium uptake in vivo and in vitro. The in vivo technique involved the use of an uptake chamber constructed on top of the CAM in situ. The in vitro methods included two systems: CAM tissue disks and cell-free microsomal membranes isolated from the CAM. Analyses using these three assays show that calcium uptake by the CAM exhibited characteristics indicative of active transport, such as temperature dependence, saturability, energetic requirement and ion specificity. The data also show that calcium-uptake activities of the CAM increase as a function of embryonic age in a manner coincident with the increased accumulation of calcium by the developing embryo in ovo.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biological Transport, Active</subject><subject>calcium</subject><subject>Calcium - metabolism</subject><subject>characterization</subject><subject>Chick Embryo</subject><subject>chicks</subject><subject>chorioallantoic membrane</subject><subject>embryo (animal)</subject><subject>embryogenesis</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Embryonic and Fetal Development</subject><subject>Extraembryonic Membranes - metabolism</subject><subject>Extraembryonic Membranes - ultrastructure</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>in vitro</subject><subject>in vivo studies</subject><subject>Microscopy, Electron</subject><subject>Microsomes - ultrastructure</subject><subject>nutrient transport</subject><subject>Organogenesis. Fetal development</subject><subject>Organogenesis. 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I. In vivo and in vitro characterization</title><author>Tuan, R.S ; Carson, M.J ; Jozefiak, J.A ; Knowles, K.A ; Shotwell, B.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c285t-186c242323c4b224d74ed3932cdec5932b600275fcf873436b89ea003a04e53a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biological Transport, Active</topic><topic>calcium</topic><topic>Calcium - metabolism</topic><topic>characterization</topic><topic>Chick Embryo</topic><topic>chicks</topic><topic>chorioallantoic membrane</topic><topic>embryo (animal)</topic><topic>embryogenesis</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Embryonic and Fetal Development</topic><topic>Extraembryonic Membranes - metabolism</topic><topic>Extraembryonic Membranes - ultrastructure</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>in vitro</topic><topic>in vivo studies</topic><topic>Microscopy, Electron</topic><topic>Microsomes - ultrastructure</topic><topic>nutrient transport</topic><topic>Organogenesis. Fetal development</topic><topic>Organogenesis. Physiological fonctions</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tuan, R.S</creatorcontrib><creatorcontrib>Carson, M.J</creatorcontrib><creatorcontrib>Jozefiak, J.A</creatorcontrib><creatorcontrib>Knowles, K.A</creatorcontrib><creatorcontrib>Shotwell, B.A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cell science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tuan, R.S</au><au>Carson, M.J</au><au>Jozefiak, J.A</au><au>Knowles, K.A</au><au>Shotwell, B.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Calcium-transport function of the chick embryonic chorioallantoic membrane. I. In vivo and in vitro characterization</atitle><jtitle>Journal of cell science</jtitle><addtitle>J Cell Sci</addtitle><date>1986-06</date><risdate>1986</risdate><volume>82</volume><issue>1</issue><spage>73</spage><epage>84</epage><pages>73-84</pages><issn>0021-9533</issn><eissn>1477-9137</eissn><coden>JNCSAI</coden><abstract>During chick embryonic development, the chorioallantoic membrane (CAM) is responsible for the mobilization of shell calcium into the embryonic circulation. The calcium-transport function of the CAM was studied here by measuring CAM calcium uptake in vivo and in vitro. The in vivo technique involved the use of an uptake chamber constructed on top of the CAM in situ. The in vitro methods included two systems: CAM tissue disks and cell-free microsomal membranes isolated from the CAM. Analyses using these three assays show that calcium uptake by the CAM exhibited characteristics indicative of active transport, such as temperature dependence, saturability, energetic requirement and ion specificity. The data also show that calcium-uptake activities of the CAM increase as a function of embryonic age in a manner coincident with the increased accumulation of calcium by the developing embryo in ovo.</abstract><cop>Cambridge</cop><pub>Company of Biologists</pub><pmid>3793786</pmid><doi>10.1242/jcs.82.1.73</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9533 |
| ispartof | Journal of cell science, 1986-06, Vol.82 (1), p.73-84 |
| issn | 0021-9533 1477-9137 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek; Company of Biologists |
| subjects | Animals Biological and medical sciences Biological Transport, Active calcium Calcium - metabolism characterization Chick Embryo chicks chorioallantoic membrane embryo (animal) embryogenesis Embryology: invertebrates and vertebrates. Teratology Embryonic and Fetal Development Extraembryonic Membranes - metabolism Extraembryonic Membranes - ultrastructure Fundamental and applied biological sciences. Psychology in vitro in vivo studies Microscopy, Electron Microsomes - ultrastructure nutrient transport Organogenesis. Fetal development Organogenesis. Physiological fonctions |
| title | Calcium-transport function of the chick embryonic chorioallantoic membrane. I. In vivo and in vitro characterization |
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