DNA conformational dynamics in polymer solutions above and below the entanglement limit
Video microscopy of nucleic acids (DNA) undergoing electrophoresis in hydroxyethyl cellulose (HEC) sieving buffers demonstrates previously unobserved shape-changing interactions between DNA and HEC molecules. We provide the first visual demonstration of entanglement between DNA and one or several di...
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| Veröffentlicht in: | Analytical chemistry (Washington) 1995-03, Vol.67 (6), p.1132-1138 |
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| container_title | Analytical chemistry (Washington) |
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| creator | Shi, Xuelong Hammond, Richard W. Morris, Michael D. |
| description | Video microscopy of nucleic acids (DNA) undergoing electrophoresis in hydroxyethyl cellulose (HEC) sieving buffers demonstrates previously unobserved shape-changing interactions between DNA and HEC molecules. We provide the first visual demonstration of entanglement between DNA and one or several discrete HEC molecules, which has been postulated to occur in ultradilute polymer solutions. Typically, nucleic acids appear to become entangled with HEC at a single region only, in both dilute and fully entangled HEC solutions. Fluctuations of the center of mass velocity of a DNA molecule and its correlation with conformation are revealed from analyses of the image data. These observations account for the success of recently reported rapid, high-resolution dc and pulsed-field capillary electrophoretic separations of nucleic acids in ultradilute hydroxyethyl cellulose solutions and hydroxyethyl cellulose/poly(ethylene oxide) solutions. |
| doi_str_mv | 10.1021/ac00102a018 |
| format | Article |
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We provide the first visual demonstration of entanglement between DNA and one or several discrete HEC molecules, which has been postulated to occur in ultradilute polymer solutions. Typically, nucleic acids appear to become entangled with HEC at a single region only, in both dilute and fully entangled HEC solutions. Fluctuations of the center of mass velocity of a DNA molecule and its correlation with conformation are revealed from analyses of the image data. 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Chem</addtitle><description>Video microscopy of nucleic acids (DNA) undergoing electrophoresis in hydroxyethyl cellulose (HEC) sieving buffers demonstrates previously unobserved shape-changing interactions between DNA and HEC molecules. We provide the first visual demonstration of entanglement between DNA and one or several discrete HEC molecules, which has been postulated to occur in ultradilute polymer solutions. Typically, nucleic acids appear to become entangled with HEC at a single region only, in both dilute and fully entangled HEC solutions. Fluctuations of the center of mass velocity of a DNA molecule and its correlation with conformation are revealed from analyses of the image data. 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Psychology</subject><subject>Microscopy, Fluorescence</subject><subject>Microscopy, Video</subject><subject>Molecules</subject><subject>Nucleic Acid Conformation</subject><subject>Nucleic acids</subject><subject>Polymers</subject><subject>Polymers - chemistry</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Solutions</subject><subject>Thermodynamics</subject><subject>Viscosity</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0N9rFDEQB_AgSj2rTz4LQYo-yGp-bDJ7j6W2KpZT9KDQlzCbzerWbHImu-r996bccYr4lIHvh5nMEPKYs5ecCf4KLWOlQMabO2TBlWCVbhpxlywYY7ISwNh98iDnm8I44_qIHAFwUEIvyNXr1Sm1MfQxjTgNMaCn3TbgONhMh0A30W9Hl2iOfr6NM8U2_nAUQ0db5-NPOn111IUJwxfvxlJQP4zD9JDc69Fn92j_HpP1xfn67G11-eHNu7PTywpr0FOle6Gtq2XHFcieO2n1snFaaQ66bVgnGegCcIkCFbiudkxAvVSA0HWtksfk2a7tJsXvs8uTGYdsnfcYXJyzARBcM5AFPv0H3sQ5lW2zERwaVZeBBb3YIZtizsn1ZpOGEdPWcGZuT23-OnXRT_Yt53Z03cHub1vyk32O2aLvEwY75AOTteIArLBqx4Y8uV-HGNM3o0GCMuuPn821uhLvV59W5rr45zuPNv9Z4n8f_A3sraCh</recordid><startdate>19950315</startdate><enddate>19950315</enddate><creator>Shi, Xuelong</creator><creator>Hammond, Richard W.</creator><creator>Morris, Michael D.</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19950315</creationdate><title>DNA conformational dynamics in polymer solutions above and below the entanglement limit</title><author>Shi, Xuelong ; Hammond, Richard W. ; Morris, Michael D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a476t-6f26ce43d1573f1e3c698e656176b80d30766cea9a2a57ed4e0274957a7ddb53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biochemistry</topic><topic>Biological and medical sciences</topic><topic>Cellulose - analogs & derivatives</topic><topic>Cellulose - chemistry</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Dna, deoxyribonucleoproteins</topic><topic>DNA, Fungal - chemistry</topic><topic>Electrophoresis - methods</topic><topic>Fundamental and applied biological sciences. 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Chem</addtitle><date>1995-03-15</date><risdate>1995</risdate><volume>67</volume><issue>6</issue><spage>1132</spage><epage>1138</epage><pages>1132-1138</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>Video microscopy of nucleic acids (DNA) undergoing electrophoresis in hydroxyethyl cellulose (HEC) sieving buffers demonstrates previously unobserved shape-changing interactions between DNA and HEC molecules. We provide the first visual demonstration of entanglement between DNA and one or several discrete HEC molecules, which has been postulated to occur in ultradilute polymer solutions. Typically, nucleic acids appear to become entangled with HEC at a single region only, in both dilute and fully entangled HEC solutions. Fluctuations of the center of mass velocity of a DNA molecule and its correlation with conformation are revealed from analyses of the image data. These observations account for the success of recently reported rapid, high-resolution dc and pulsed-field capillary electrophoretic separations of nucleic acids in ultradilute hydroxyethyl cellulose solutions and hydroxyethyl cellulose/poly(ethylene oxide) solutions.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>7717526</pmid><doi>10.1021/ac00102a018</doi><tpages>7</tpages></addata></record> |
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| ispartof | Analytical chemistry (Washington), 1995-03, Vol.67 (6), p.1132-1138 |
| issn | 0003-2700 1520-6882 |
| language | eng |
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| source | MEDLINE; American Chemical Society Journals |
| subjects | Analytical, structural and metabolic biochemistry Biochemistry Biological and medical sciences Cellulose - analogs & derivatives Cellulose - chemistry Deoxyribonucleic acid DNA Dna, deoxyribonucleoproteins DNA, Fungal - chemistry Electrophoresis - methods Fundamental and applied biological sciences. Psychology Microscopy, Fluorescence Microscopy, Video Molecules Nucleic Acid Conformation Nucleic acids Polymers Polymers - chemistry Saccharomyces cerevisiae - genetics Solutions Thermodynamics Viscosity |
| title | DNA conformational dynamics in polymer solutions above and below the entanglement limit |
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