Detection and cloning of a common region of loss of heterozygosity at chromosome 1p in breast cancer
The short arm of chromosome 1 is frequently affected by rearrangements in a variety of human malignancies. Genetic alterations, predominantly deletions, which are indicative of the presence of a putative tumor suppressor gene at chromosome 1p, are observed in breast cancer. In order to define the al...
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| Veröffentlicht in: | Cancer research (Chicago, Ill.) Ill.), 1995-04, Vol.55 (8), p.1752-1757 |
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| container_title | Cancer research (Chicago, Ill.) |
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| creator | NAGAI, H NEGRINI, M CARTER, S. L GILLUM, D. R ROSENBERG, A. L SCHWARTZ, G. F CROCE, C. M |
| description | The short arm of chromosome 1 is frequently affected by rearrangements in a variety of human malignancies. Genetic alterations, predominantly deletions, which are indicative of the presence of a putative tumor suppressor gene at chromosome 1p, are observed in breast cancer. In order to define the altered locus, eleven highly polymorphic microsatellite markers on chromosome 1p were used to detect loss of heterozygosity. We analyzed 52 cases of breast cancer and found 4 common deleted regions at chromosome 1p. Twenty-two of 52 (42%) informative patients showed at least 1 affected locus. The region most frequently exhibiting loss of heterozygosity was 1p31 (11/39; 28%); the other three common deleted regions were 1p36 (10/44; 23%), 1p35-36 (5/40; 13%), and 1p13 (8/39; 21%). These data suggest that one or more putative tumor suppressor genes may reside on chromosome 1p. We have cloned the entire region of interest at 1p31 in yeast artificial chromosomes. This yeast artificial chromosome contig can be used for fine mapping of the region and cloning of the candidate tumor suppressor gene. |
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L ; GILLUM, D. R ; ROSENBERG, A. L ; SCHWARTZ, G. F ; CROCE, C. M</creator><creatorcontrib>NAGAI, H ; NEGRINI, M ; CARTER, S. L ; GILLUM, D. R ; ROSENBERG, A. L ; SCHWARTZ, G. F ; CROCE, C. M</creatorcontrib><description>The short arm of chromosome 1 is frequently affected by rearrangements in a variety of human malignancies. Genetic alterations, predominantly deletions, which are indicative of the presence of a putative tumor suppressor gene at chromosome 1p, are observed in breast cancer. In order to define the altered locus, eleven highly polymorphic microsatellite markers on chromosome 1p were used to detect loss of heterozygosity. We analyzed 52 cases of breast cancer and found 4 common deleted regions at chromosome 1p. Twenty-two of 52 (42%) informative patients showed at least 1 affected locus. The region most frequently exhibiting loss of heterozygosity was 1p31 (11/39; 28%); the other three common deleted regions were 1p36 (10/44; 23%), 1p35-36 (5/40; 13%), and 1p13 (8/39; 21%). These data suggest that one or more putative tumor suppressor genes may reside on chromosome 1p. We have cloned the entire region of interest at 1p31 in yeast artificial chromosomes. This yeast artificial chromosome contig can be used for fine mapping of the region and cloning of the candidate tumor suppressor gene.</description><identifier>ISSN: 0008-5472</identifier><identifier>EISSN: 1538-7445</identifier><identifier>PMID: 7712484</identifier><identifier>CODEN: CNREA8</identifier><language>eng</language><publisher>Philadelphia, PA: American Association for Cancer Research</publisher><subject>Base Sequence ; Biological and medical sciences ; Breast - pathology ; Breast Neoplasms - genetics ; Breast Neoplasms - pathology ; Chromosome Deletion ; Chromosome Mapping ; Chromosomes, Artificial, Yeast ; Chromosomes, Human, Pair 1 ; Cloning, Molecular ; DNA Primers ; Female ; Genetic Markers ; Gynecology. Andrology. Obstetrics ; Humans ; Mammary gland diseases ; Medical sciences ; Molecular Sequence Data ; Polymerase Chain Reaction ; Repetitive Sequences, Nucleic Acid ; Tumors</subject><ispartof>Cancer research (Chicago, Ill.), 1995-04, Vol.55 (8), p.1752-1757</ispartof><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3523494$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7712484$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>NAGAI, H</creatorcontrib><creatorcontrib>NEGRINI, M</creatorcontrib><creatorcontrib>CARTER, S. L</creatorcontrib><creatorcontrib>GILLUM, D. R</creatorcontrib><creatorcontrib>ROSENBERG, A. L</creatorcontrib><creatorcontrib>SCHWARTZ, G. F</creatorcontrib><creatorcontrib>CROCE, C. M</creatorcontrib><title>Detection and cloning of a common region of loss of heterozygosity at chromosome 1p in breast cancer</title><title>Cancer research (Chicago, Ill.)</title><addtitle>Cancer Res</addtitle><description>The short arm of chromosome 1 is frequently affected by rearrangements in a variety of human malignancies. Genetic alterations, predominantly deletions, which are indicative of the presence of a putative tumor suppressor gene at chromosome 1p, are observed in breast cancer. In order to define the altered locus, eleven highly polymorphic microsatellite markers on chromosome 1p were used to detect loss of heterozygosity. We analyzed 52 cases of breast cancer and found 4 common deleted regions at chromosome 1p. Twenty-two of 52 (42%) informative patients showed at least 1 affected locus. The region most frequently exhibiting loss of heterozygosity was 1p31 (11/39; 28%); the other three common deleted regions were 1p36 (10/44; 23%), 1p35-36 (5/40; 13%), and 1p13 (8/39; 21%). These data suggest that one or more putative tumor suppressor genes may reside on chromosome 1p. We have cloned the entire region of interest at 1p31 in yeast artificial chromosomes. This yeast artificial chromosome contig can be used for fine mapping of the region and cloning of the candidate tumor suppressor gene.</description><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Breast - pathology</subject><subject>Breast Neoplasms - genetics</subject><subject>Breast Neoplasms - pathology</subject><subject>Chromosome Deletion</subject><subject>Chromosome Mapping</subject><subject>Chromosomes, Artificial, Yeast</subject><subject>Chromosomes, Human, Pair 1</subject><subject>Cloning, Molecular</subject><subject>DNA Primers</subject><subject>Female</subject><subject>Genetic Markers</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Mammary gland diseases</subject><subject>Medical sciences</subject><subject>Molecular Sequence Data</subject><subject>Polymerase Chain Reaction</subject><subject>Repetitive Sequences, Nucleic Acid</subject><subject>Tumors</subject><issn>0008-5472</issn><issn>1538-7445</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kEtLAzEUhYMotVZ_gpCFuBvI46bJLKU-oeBG10MmyUwjk0lNpov6601xcHW43zn3wrlnaEkFV5UEEOdoSQhRlQDJLtFVzl9lFJSIBVpISRkoWCL76CZnJh9HrEeLzRBHP_Y4dlhjE0MoPLn-ZBc0xJxPuis7Kf4c-5j9dMR6wmaXYog5BofpHvsRt8npXLgejUvX6KLTQ3Y3s67Q5_PTx-a12r6_vG0ettWOrdVU0ZYqJSW0NayNbevaGeispUzxggmtKQirmWa8ltZRkLwGYNKsFQAlUvIVuv-7u0_x--Dy1ASfjRsGPbp4yI2UjAKRpARv5-ChDc42--SDTsdmfkvx72ZfZ6OHLpUaPv_HuGAcauC_YmBrGA</recordid><startdate>19950415</startdate><enddate>19950415</enddate><creator>NAGAI, H</creator><creator>NEGRINI, M</creator><creator>CARTER, S. 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M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h268t-1b188774b946cdb99ec4fdd1283877019145da2a2397de147394427c684410773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Breast - pathology</topic><topic>Breast Neoplasms - genetics</topic><topic>Breast Neoplasms - pathology</topic><topic>Chromosome Deletion</topic><topic>Chromosome Mapping</topic><topic>Chromosomes, Artificial, Yeast</topic><topic>Chromosomes, Human, Pair 1</topic><topic>Cloning, Molecular</topic><topic>DNA Primers</topic><topic>Female</topic><topic>Genetic Markers</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Mammary gland diseases</topic><topic>Medical sciences</topic><topic>Molecular Sequence Data</topic><topic>Polymerase Chain Reaction</topic><topic>Repetitive Sequences, Nucleic Acid</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>NAGAI, H</creatorcontrib><creatorcontrib>NEGRINI, M</creatorcontrib><creatorcontrib>CARTER, S. L</creatorcontrib><creatorcontrib>GILLUM, D. R</creatorcontrib><creatorcontrib>ROSENBERG, A. L</creatorcontrib><creatorcontrib>SCHWARTZ, G. F</creatorcontrib><creatorcontrib>CROCE, C. 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M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection and cloning of a common region of loss of heterozygosity at chromosome 1p in breast cancer</atitle><jtitle>Cancer research (Chicago, Ill.)</jtitle><addtitle>Cancer Res</addtitle><date>1995-04-15</date><risdate>1995</risdate><volume>55</volume><issue>8</issue><spage>1752</spage><epage>1757</epage><pages>1752-1757</pages><issn>0008-5472</issn><eissn>1538-7445</eissn><coden>CNREA8</coden><abstract>The short arm of chromosome 1 is frequently affected by rearrangements in a variety of human malignancies. Genetic alterations, predominantly deletions, which are indicative of the presence of a putative tumor suppressor gene at chromosome 1p, are observed in breast cancer. In order to define the altered locus, eleven highly polymorphic microsatellite markers on chromosome 1p were used to detect loss of heterozygosity. We analyzed 52 cases of breast cancer and found 4 common deleted regions at chromosome 1p. Twenty-two of 52 (42%) informative patients showed at least 1 affected locus. The region most frequently exhibiting loss of heterozygosity was 1p31 (11/39; 28%); the other three common deleted regions were 1p36 (10/44; 23%), 1p35-36 (5/40; 13%), and 1p13 (8/39; 21%). These data suggest that one or more putative tumor suppressor genes may reside on chromosome 1p. We have cloned the entire region of interest at 1p31 in yeast artificial chromosomes. This yeast artificial chromosome contig can be used for fine mapping of the region and cloning of the candidate tumor suppressor gene.</abstract><cop>Philadelphia, PA</cop><pub>American Association for Cancer Research</pub><pmid>7712484</pmid><tpages>6</tpages></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; American Association for Cancer Research |
| subjects | Base Sequence Biological and medical sciences Breast - pathology Breast Neoplasms - genetics Breast Neoplasms - pathology Chromosome Deletion Chromosome Mapping Chromosomes, Artificial, Yeast Chromosomes, Human, Pair 1 Cloning, Molecular DNA Primers Female Genetic Markers Gynecology. Andrology. Obstetrics Humans Mammary gland diseases Medical sciences Molecular Sequence Data Polymerase Chain Reaction Repetitive Sequences, Nucleic Acid Tumors |
| title | Detection and cloning of a common region of loss of heterozygosity at chromosome 1p in breast cancer |
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